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Thermo Scientific™ Taq DNA Polymerase, Recombinant


Optimize routine PCR with this highly thermostable DNA polymerase.

Manufacturer: thermo scientific™  EP0405

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 Disclaimers

In certain countries use of this product is covered by patents. Purchase of product in these countries includes non-transferable, limited license for using only this amount of product for the purchaser's own internal research.

Catalog No. FEREP0405

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Description & Specifications

Specifications

For Use With (Application) Routine PCR amplification of DNA fragments up to 5kb (1); Generation of PCR product for TA cloning; DNA labeling (2–4); DNA sequencing (5)
Includes 10 x 1.25mL of 25mM MgCl2, 10 x 1.25mL of 10X Taq Buffer with KCl, 10 x 1.25mL of 10X Taq Buffer with (NH4)2SO4
Overhang Generates PCR products with 3'-dA overhangs
Product Name Taq DNA Polymerase (recombinant) (5 U/µL)
Quantity 5 x 500 Units
Thermostability half life is more than 40 min. at 95°C

Optimize routine PCR with this highly thermostable DNA polymerase.

Thermo Scientific ™ Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme catalyzes 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, Taq DNA Polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Recombinant Taq DNA Polymerase is the ideal tool for standard PCR of templates 5 kb or shorter.

PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimized for efficient and reproducible PCR.

Highlights

  • Thermostable -half life is more than 40 min at 95°C
  • Generates PCR products with 3'-dA overhangs
  • Supplied with two buffers -10X Taq Buffer with KCl and 10X Taq Buffer with (NH4)2SO4. The latter allows for PCR at wide range of magnesium concentrations and decreases unspecific priming
  • Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)