Standard PCR Reagents and Kits

Standard PCR Reagents and Kits

Products for use during standard PCR procedures. Includes master mixes, DNA polymerases, reverse transcription mixes, PCR beads, buffers, and kits. Ideal when specificity; precise quantification; and high fidelity, sensitivity, and yield are not requirements.
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1

Thermo Scientific™ DreamTaq Green PCR Master Mix (2X)

Save time in PCR applications with Master Mix and get higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerase.

Polymerase DreamTaq DNA Polymerase
GC-Rich PCR Performance Low
Fidelity (vs. Taq) 1 X
Reaction Format SuperMix or Master Mix
Reaction Speed Standard
Hot Start No
Overhang 3'-A
Product Line DreamTaq™
2

Invitrogen™ Taq DNA Polymerase, recombinant  DFS Item

A thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer

GC-Rich PCR Performance Low
Fidelity (vs. Taq) 1 X
Overhang 3'-A
Concentration 5 U/µL
PCR Method qPCR,Standard PCR
Exonuclease Activity 5' - 3'
Detection Method Primer-probe
Polymerase Taq DNA Polymerase
Format Stand-alone enzyme
Size (Final Product) 5 kb or less
Reaction Format Separate Components
Product Type DNA Polymerase
Reaction Speed Standard
Hot Start No
Starting Material DNA
4

MilliporeSigma™ Novagen™ NovaTaq™ Hot Start Master Mix Kits

Premixed 2X hot start PCR components for convenience and reproducibility

Content And Storage Ready-to-use 2X mixture of NovaTaq Hot Start DNA Polymerase, ultrapure deoxynucleotides, and reaction buffer with MgCl2, PCR Grade Water, MgCl2 for optimizing Mg2+ concentrations
Product Type Hot start master mix kit
5

Applied Biosystems™ MagMAX™ Pure Bind Beads

MagMAX Pure Bind Beads are ready-to-use magnetic beads that provide optimized size selection and PCR cleanup, removing small fragments such as dNTPs, salts, primers, and primer-dimers.

GC-Rich PCR Performance High
Form Beads in Suspension
Reaction Speed 20 min.
For Use With (Application) NGS Cleanup,PCR Cleanup,PCR Purification,DNA Purification,Sequencing
6

Invitrogen™ PCR SuperMix  DFS Item

Ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification

7

Invitrogen™ Taq DNA Polymerase, native  DFS Item

A thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer

GC-Rich PCR Performance Low
Fidelity (vs. Taq) 1 X
Overhang 3'-A
Concentration 5 U/µL
Exonuclease Activity 5' - 3'
Detection Method Primer-probe
Polymerase Taq DNA Polymerase
Format Stand-alone enzyme
Size (Final Product) 5 kb or less
Reaction Format Separate Components
Product Type DNA Polymerase
Reaction Speed Standard
Hot Start No
Starting Material DNA
8

Applied Biosystems™ TaqMan™ Sample-to-SNP™ Kit (200mL Sample prep, 10mL PCR)  DFS Item

Go from Biological Sample to Genotyping Results in less than one hour.

Promotions are available
Sample Type Buccal Sample, Cell Cultures, Hair with Follicle, Leaf Punch or Needle, Seed Chip, Tail Biopsy (Mouse), Tail Biopsy (Rat), Tissue (FFPE), Whole Blood
Reaction Speed Fast or Standard
Detection Method Primer-probe
9

Cytiva PuReTaq Ready-To-Go™ PCR Beads

Pre-mixed, pre-dispensed reaction beads optimized for performing standard PCR amplifications.  Cytiva Lifescience™ illustra™ PuReTaq Ready-To-Go™ PCR Beads are stable at ambient temperature.

Promotions are available
Content And Storage Room temperature stable beads containing ∼ 2.5 units of recombinant PuReTaq DNA Polymerase, dATP, dCTP, dGTP, dTTP, stabilizers, BSA and reaction Buffer, When a bead is reconstituted to a 25μL final volume, the concentration of each dNTP
Quantity 1 Kit
For Use With (Application) Standard PCR
10

Cytiva Hot Start Master Mix

A ready-to-use 2X mastermix providing robust and reliable performance for demanding PCR applications in which high specificity and high sensitivity are essential to success

11

Cytiva Taq DNA Polymerase (cloned)

For PCR and other procedures requiring DNA polymerase activity at elevated temperatures

Content And Storage 100mM Tris HCl, pH 9, 15mM MgCl2 and 500mM KCl. Store at -20°C
Purity or Quality Grade Free from detectable nonspecific nuclease and Taq I restriction endonuclease activities.
pH 7.5
Concentration 5000U/mL
For Use With (Application) Standard PCR
12

Applied Biosystems™ TaqMan™ DNA Template Reagents  DFS Item

Provide the necessary components to perform a standard-dilution series with the beta-actin gene using nucleic acid from human cells

Promotions are available
13

Applied Biosystems™ GeneAmp™ 10X PCR Buffer I, (2 x 75 mL)  DFS Item

Optimized for use with AmpliTaq™ DNA Polymerase for robust PCR amplification

14

Applied Biosystems™ GeneAmp™ 10X PCR Gold Buffer & MgCl2  DFS Item

Polymerase AmpliTaq Gold DNA Polymerase
Format Kit
Product Type 10X PCR Gold Buffer
Concentration 10X
Product Line GeneAmp™
Detection Method Primer-probe
15

Applied Biosystems™ AmpliTaq™ 360 Buffer, 25 mM MgCl2 and 360 GC Enhancer  DFS Item

Use with AmpliTaq 360 DNA polymerase to supplement reagents for experiments when needed