Standard PCR Reagents and Kits

Standard PCR Reagents and Kits
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Invitrogen™ Taq DNA Polymerase, recombinant
Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.
Shipping Condition | Dry Ice |
---|---|
GC-Rich PCR Performance | Low |
Fidelity (vs. Taq) | 1X |
Overhang | 3'-A |
Concentration | 5 U/μL |
For Use With (Application) | Standard PCR |
PCR Method | qPCR,Standard PCR |
Exonuclease Activity | 5' - 3' |
Polymerase | Taq DNA Polymerase |
Format | Stand-alone enzyme |
Size (Final Product) | 5 kb or less |
Reaction Format | Separate Components |
Product Type | DNA Polymerase |
Reaction Speed | Standard |
Hot Start | No |
Starting Material | DNA |
Thermo Scientific™ DreamTaq PCR Master Mixes (2X)
Thermo Scientific DreamTaq PCR Master Mix (2X) is a ready-to-use solution containing DreamTaq DNA Polymerase, optimized DreamTaq buffer, MgCl2, and dNTPs.
Shipping Condition | Dry Ice |
---|---|
Polymerase | DreamTaq DNA Polymerase |
GC-Rich PCR Performance | Low |
Fidelity (vs. Taq) | 1X |
Reaction Format | SuperMix or Master Mix |
Reaction Speed | Standard |
Hot Start | No |
Overhang | 3'-A |
For Use With (Application) | Standard PCR |
Invitrogen™ Taq DNA Polymerase, native
Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.
Shipping Condition | Wet or Dry Ice |
---|---|
GC-Rich PCR Performance | Low |
Fidelity (vs. Taq) | 1X |
Overhang | 3'-A |
Concentration | 5 U/μL |
For Use With (Application) | Standard PCR |
Exonuclease Activity | 5' - 3' |
Polymerase | Taq DNA Polymerase |
Format | Stand-alone enzyme |
Size (Final Product) | 5 kb or less |
Reaction Format | Separate Components |
Product Type | DNA Polymerase |
Reaction Speed | Standard |
Hot Start | No |
Starting Material | DNA |
Thermo Scientific™ PCR Master Mix (2X)
Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers.
Polymerase | Taq DNA Polymerase |
---|---|
GC-Rich PCR Performance | Low |
Fidelity (vs. Taq) | 1X |
Reaction Format | SuperMix or Master Mix |
Product Type | PCR Master Mix |
Reaction Speed | Standard |
Hot Start | No |
Overhang | 3'-A |
Concentration | 2X |
Starting Material | DNA |
For Use With (Application) | Standard PCR |
Invitrogen™ PCR SuperMix
PCR SuperMix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification.
Thermo Scientific™ Taq DNA Polymerase, recombinant (5 U/μL)
Thermo Scientific Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus.
Shipping Condition | Dry Ice |
---|---|
Polymerase | Taq DNA Polymerase |
GC-Rich PCR Performance | Low |
Fidelity (vs. Taq) | 1X |
Reaction Format | Separate Components |
Product Type | Taq DNA Polymerase (Recombinant) |
Reaction Speed | Standard |
Hot Start | No |
Overhang | 3'-A |
Concentration | 5 U/μL |
For Use With (Application) | Standard PCR |
Thermo Scientific™ DreamTaq DNA Polymerase (5 U/μL)
Thermo Scientific DreamTaq DNA Polymerase is an enhanced Taq DNA polymerase optimized for all standard PCR applications. It ensures higher sensitivity, longer PCR products, and higher yields compared to conventional Taq DNA polymerase.
Polymerase | DreamTaq DNA Polymerase |
---|---|
GC-Rich PCR Performance | Low |
Fidelity (vs. Taq) | 1X |
Reaction Speed | Standard |
Hot Start | No |
Overhang | 3'-A |
Concentration | 5 U/μL |
Applied Biosystems™ AmpliTaq™ DNA Polymerase, LD (Low DNA)
Applied Biosystems AmpliTaq DNA Polymerase, LD (Low DNA), is the same enzyme as AmpliTaq DNA Polymerase, but is further purified through a proprietary separation process to ensure that residual bacterial DNA sequences are substantially reduced.
Shipping Condition | Dry Ice |
---|---|
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 1X |
Purity or Quality Grade | LD (Low DNA) |
Quantity | 250 units |
Overhang | 3'-A |
Concentration | 5 U/μL |
For Use With (Application) | Standard PCR |
Exonuclease Activity | 5' - 3' |
Polymerase | AmpliTaq DNA Polymerase |
No. of Reactions | 200 Reactions |
Format | Stand-alone enzyme |
Size (Final Product) | 5 kb or less |
Reaction Format | Separate Components |
Product Type | DNA Polymerase |
Reaction Speed | Standard |
Hot Start | No |
Starting Material | DNA |
Applied Biosystems™ AmpErase™ Uracil N-Glycosylase (UNG)
Enzyme removes any uracil incorporated into single- or double-stranded DNA
Applied Biosystems™ GeneAmp™ 10X PCR Buffer II & MgCl2
Applied Biosystems GeneAmp 10X PCR Buffer II is optimized for use with AmpliTaq DNA Polymerase, resulting in robust PCR amplification.
Polymerase | AmpliTaq DNA Polymerase |
---|---|
Fidelity (vs. Taq) | 1X |
Reaction Format | Kit |
Product Type | PCR Buffer |
Concentration | 10X |
For Use With (Application) | Standard PCR |
Applied Biosystems™ MicroSEQ™ Full Gene 16S rDNA PCR Kit
The MicroSEQ Full Gene 16S rDNA PCR Kit is the PCR component of the MicroSEQ 16S rDNA Full Gene Identification Kit, which provides an easy-to-use DNA sequence-based method to identify most bacteria.
Applied Biosystems™ AmpliTaq™ 360 Buffer, 25 mM MgCl2 and 360 GC Enhancer
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The Applied Biosystems AmpliTaq 360 Buffer Kit is used in conjection with the AmpliTaq 360 DNA polymerase for those few experiments when the reagents supplied with the enzyme are not sufficient for the researcher's needs.
Applied Biosystems™ AmpliTaq™ 360 DNA Polymerase
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The Applied Biosystems AmpliTaq360 DNA Polymerase, when used with the new enhanced AmpliTaq 360 Buffer and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts.
Fidelity (vs. Taq) | 1X |
---|---|
Format | Stand-alone enzyme |
Size (Final Product) | 5 kb or less |
Reaction Format | Separate Components |
Overhang | 3'-A |
Concentration | 5 U/μL |
Starting Material | DNA |
Exonuclease Activity | 5' - 3' |
Applied Biosystems™ TaqMan™ DNA Template Reagents
Provide the necessary components to perform a standard-dilution series with the beta-actin gene using nucleic acid from human cells
Applied Biosystems™ GeneAmp™ 10X PCR Gold Buffer & MgCl2
Applied Biosystems GeneAmp 10X PCR Gold Buffer is formulated to provide flexible, efficient activation of AmpliTaq Gold DNA Polymerase, resulting in highly specific, robust PCR amplification.
Polymerase | AmpliTaq Gold DNA Polymerase |
---|---|
Format | Kit |
Product Type | 10X PCR Gold Buffer |
Concentration | 10X |
For Use With (Application) | Standard PCR |