Standard PCR Reagents and Kits

Standard PCR Reagents and Kits

Products for use during standard PCR procedures. Includes master mixes, DNA polymerases, reverse transcription mixes, PCR beads, buffers, and kits. Ideal when specificity; precise quantification; and high fidelity, sensitivity, and yield are not requirements.
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1 – 30 of 45 results
1

MilliporeSigma™ Novagen™ NovaTaq™ Hot Start Master Mix Kits

Premixed 2X hot start PCR components for convenience and reproducibility

Includes Ready-to-use 2X mixture of NovaTaq Hot Start DNA Polymerase, ultrapure deoxynucleotides, and reaction buffer with MgCl2, PCR Grade Water, MgCl2 for optimizing Mg2+ concentrations
Product Type Hot start master mix kit
2

Cytiva RAPD Analysis Beads

For performing DNA-profiling experiments using the randomly amplified polymorphic DNA (RAPD) technique

No. of Reactions 100
Format 0.5mL Tube
Volume 500μL
Color Colorless
pH 8.3
Quantity 1 Kit
3

Cytiva Hot Start Master Mix

A ready-to-use 2X mastermix providing robust and reliable performance for demanding PCR applications in which high specificity and high sensitivity are essential to success

4

Cytiva Ready-To-Go™ RT-PCR Beads

Optimized for performing either one-step or conventional two-step reverse transcription.  Cytiva Lifescience™ Illustra™ Ready-To-Go™ RT-PCR Beads are ambient temperature stable, pre-mixed, and ready for pre-dispensed reactions.

Includes Room temperature stable beads containing Buffer, dATP, dCTP, dGTP, dTTP, approximately 2 units of Taq DNA Polymerase, M-MLV reverse Transcriptase, RNAguard (porcine) and stabilizers (including RNase/DNase free BSA), When a bead is reconstituted to 50
Quantity 1 Kit
Storage Requirements Ambient in pouch with desiccant
5

Thermo Scientific™ Taq DNA Polymerase, native, without BSA (5 U/µL)

Optimize amplification of DNA samples of low purity with this highly thermostable, native DNA polymerase for routine PCR.

6

Invitrogen™ PCR SuperMix  DFS Item

Ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification

GC-Rich PCR Performance Low
Format SuperMix or Master Mix
Product Type PCR SuperMix
Exonuclease Activity 5' - 3'
7

Invitrogen™ Taq DNA Polymerase, recombinant  DFS Item

A thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer

Polymerase Taq DNA Polymerase
Format Stand-alone enzyme
Reaction Format Separate Components
Hot Start No
Overhang 3'-A
Concentration 5 U/µL
8

Invitrogen™ Taq DNA Polymerase PCR Buffer (10X)  DFS Item

A 10X buffer [200 mM Tris HCl (pH 8.4), 500 mM KCl]

9

Invitrogen™ Taq DNA Polymerase, native  DFS Item

A thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer

Format Stand-alone enzyme
Concentration 5 U/µL
Starting Material DNA
PCR Method PCR
Detection Method Primer-Probe
10

Cytiva Hot Start Mix RTG

Premixed, predispensed, ambient temperature-stable formulation providing robust and reliable performance for demanding PCR applications in which high specificity and high sensitivity are essential to success

Includes 1.75 units of recombinant PuReTaq DNA Polymerase, dATP, dCTP, dGTP, dTTP, stabilizers, BSA and reaction Buffer
Format Tube
Storage Requirements Ambient temperature in pouch with desiccant
11

Cytiva PuReTaq Ready-To-Go™ PCR Beads

Pre-mixed, pre-dispensed reaction beads optimized for performing standard PCR amplifications.  Cytiva Lifescience™ illustra™ PuReTaq Ready-To-Go™ PCR Beads are stable at ambient temperature.

Promotions are available
Includes Room temperature stable beads containing ∼ 2.5 units of recombinant PuReTaq DNA Polymerase, dATP, dCTP, dGTP, dTTP, stabilizers, BSA and reaction Buffer, When a bead is reconstituted to a 25μL final volume, the concentration of each dNTP
Color White
Quantity 1 Kit
Storage Requirements Ambient temperature in pouch with desiccant
12

Cytiva Taq DNA Polymerase (cloned)

For PCR and other procedures requiring DNA polymerase activity at elevated temperatures

Includes 100mM Tris HCl, pH 9, 15mM MgCl2 and 500mM KCl
Color Colorless
Purity Free from detectable nonspecific nuclease and Taq I restriction endonuclease activities.
pH 7.5
Storage Requirements -20°C
Concentration 5000U/mL
13

Invitrogen™ SuperScript™ IV RT-LAMP Master Mix  DFS Item

Invitrogen SuperScript IV RT-LAMP Master Mix is a solution designed for fast, sensitive, and simple RNA target detection in loop-mediated isothermal amplification (LAMP) reactions.

Polymerase Bst DNA Polymerase
Reverse Transcriptase SuperScript™ IV
Product Type Master Mix
Reaction Speed 5 to 15 minutes
Optimal Reaction Temperature 65°C
14

Applied Biosystems™ TaqMan™ DNA Template Reagents  DFS Item

Provide the necessary components to perform a standard-dilution series with the beta-actin gene using nucleic acid from human cells

Promotions are available
15

Applied Biosystems™ AmpliTaq™ 360 Buffer, 25 mM MgCl2 and 360 GC Enhancer  DFS Item

Use with AmpliTaq 360 DNA polymerase to supplement reagents for experiments when needed

16

Applied Biosystems™ AmpliTaq™ 360 DNA Polymerase  DFS Item

Amplifies vast range of DNA sequence contexts when used with enhanced AmpliTaq 360 Buffer and 360 GC Enhancer

Format Stand-alone enzyme
Overhang 3'-A
Concentration 5 U/µL
Starting Material DNA
17

Applied Biosystems™ TaqMan™ Sample-to-SNP™ Kit (200mL Sample prep, 10mL PCR)  DFS Item

Go from Biological Sample to Genotyping Results in less than one hour.

Polymerase AmpliTaq™ DNA Polymerase
Speed Fast, Standard
Sample Type Buccal Sample, Cell Cultures, Hair with Follicle, Leaf Punch or Needle, Seed Chip, Tail Biopsy (Mouse), Tail Biopsy (Rat), Tissue (FFPE), Whole Blood
Dye Type Passive Reference Dye: ROX (Pre-mixed)
Detection Method Primer-Probe Detection
18

Applied Biosystems™ TaqMan™ Sample-to-SNP™ Kit (20mL Sample prep, 50mL PCR)  DFS Item

Go from Biological Sample to Genotyping Results in less than one hour.

Polymerase AmpliTaq™ DNA Polymerase
Speed Fast, Standard
Sample Type Buccal Sample, Cell Cultures, Hair with Follicle, Leaf Punch or Needle, Seed Chip, Tail Biopsy (Mouse), Tail Biopsy (Rat), Tissue (FFPE), Whole Blood
Dye Type Passive Reference Dye: ROX (Pre-mixed)
Detection Method Primer-Probe Detection
19

Applied Biosystems™ GeneAmp™ Fast PCR Master Mix (2X), with protocol  DFS Item

Sensitive, pre-mixed hot-start chemistries

Format Tube
Product Type Fast PCR Master Mix
Hot Start Built-In Hot Start
Exonuclease Activity 5' - 3'
20

Applied Biosystems™ GeneAmp™ 10X PCR Buffer I, (2 x 75 mL)  DFS Item

Optimized for use with AmpliTaq™ DNA Polymerase for robust PCR amplification

21

Fisher BioReagents™ exACTGene™ Control Primers

Optimized for routine PCR applications

22

Thermo Scientific™ ThermoPrime Taq DNA Polymerase, with 10x buffer and separate vial of 25 mM MgCl2

For PCR applications requiring increased yield and sensitivity, these PCR kits and master mixes are optimized to produce large amounts of PCR product.

23

Applied Biosystems™ AmpliTaq™ DNA Polymerase, LD (Low DNA) with Buffer II  DFS Item

Ultrapurified AmpliTaq DNA Polymerase ensures residual bacterial DNA sequences are substantially reduced

24

Applied Biosystems™ GeneAmp™ 10X PCR Buffer  DFS Item

Optimized for use with AmpliTaq DNA Polymerase to provide robust PCR amplification

25

Applied Biosystems™ AmpErase™ Uracil N-Glycosylase (UNG)  DFS Item

Enzyme removes any uracil incorporated into single- or double-stranded DNA

26

Applied Biosystems™ AmpliTaq™ DNA Polymerase with Buffer II  DFS Item

A 94 kDa, thermostable, recombinant DNA polymerase

Polymerase AmpliTaq™ DNA Polymerase
Product Type DNA Polymerase
Concentration 5 U/μL, 10X
Starting Material DNA
Detection Method Primer-Probe
Product Line AmpliTaq™
27

Applied Biosystems™ AmpliTaq™ DNA Polymerase, LD (Low DNA) with Buffer I  DFS Item

Ultrapurified AmpliTaq DNA Polymerase ensures residual bacterial DNA sequences are substantially reduced

28

Applied Biosystems™ GeneAmp™ 10X PCR Buffer II & MgCl2  DFS Item

Optimized for use with AmpliTaq™ DNA Polymerase for robust PCR amplification

29

Applied Biosystems™ AmpliTaq™ DNA Polymerase with Buffer I  DFS Item

A 94 kDa, thermostable, recombinant DNA polymerase

Polymerase AmpliTaq™ DNA Polymerase
Format Stand-alone enzyme
Overhang 3'-A
Concentration 5 U/μL, 10X
Starting Material DNA
Product Line AmpliTaq™
30

Genlantis™ MycoScope™ PCR Detection Kit

Designed for sensitive detection of mycoplasma using PCR

Storage Requirements -20°C