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Promega™ HaloTag™ Protein Purification System

Purifies proteins fused to HaloTag, a novel protein tag that enhances the expression and solubility of recombinant proteins

$398.04 - $875.26

Specifications

Product Type HaloTag™ Protein purification system
Quantity Each
Storage Requirements 4°C
View More Specs
Includes:  Protein Purification System Sample Pack (Mfr. No. G6270) includes HaloLink Resin, 10mL of 25% slurry; HisLink Protein Purification Resin, 0.5mL; TEV Protease for use with HaloLink Resin, 200μL. Protein Purification System includes HaloLink Resin, 100mL of 25% slurry; HisLink Protein Purification Resin, 5mL; TEV Protease for use with HaloLink Resin, 1.6mL.

 Disclaimers

By use of this product, researcher agrees to be bound by the terms of this limited use statement. If the researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may only use this product in their own research and may modify or link Promega HaloTag ligands to Promega or customer-supplied moieties. No other use or transfer of this product is authorized without the express, written consent of Promega including, without limitation, commercial use. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. Promega makes no representations or warranties of any kind, either expressed or implied, including for merchantability or fitness for a particular purpose with regards to the product. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.
U.S. Patent 7,429,472 and other patents pending.
U.S. Patents 7,112,552; 7,304,015; 7,304,016; 7,354,750 and other patents pending.
Certain applications of this product may be covered by patents issued to parties other than Promega and applicable in certain countries. Because purchase of this product does not include a license to perform any of these patented applications, users of this product may be required to obtain a patent license depending upon the particular application and country in which the product is used.

Products
Catalog Number Mfr. No. Includes Price Quantity    

PRG6270

 
promega™
G6270
HaloLink™ Resin, 10mL of 25% slurry, HisLink Protein purification Resin, 0.5mL, TEV Protease for use with HaloLink™ Resin, 200μL Each for $398.04

PRG6280

 
promega™
G6280
HaloLink™ Resin, 100mL of 25% slurry, HisLink Protein purification Resin, 5mL, TEV Protease for use with HaloLink™ Resin, 1.6mL Each for $875.26
Description & Specifications

Specifications

Product Type HaloTag™ Protein purification system
Quantity Each
Storage Requirements 4°C

HaloTag Technology enables the covalent, efficient and specific capture of a protein of interest onto HaloLink™ Resin, overcoming the equilibrium-based limitations associated with affinity tags, including poor capture of proteins expressed at low levels and protein loss during washing of the purification resin.

  • Provides superior yield, purity, and specific activity of soluble, functional proteins compared to His-Tag, GST, and MBP affinity tags due to specific and covalent HaloTag fusion protein capture and immobilization on HaloLink resin
  • Permits recovery of poorly expressed fusion proteins
  • Utilizes one buffer compatible with downstream applications for all purification steps, simplifying the process and saving time
  • Suitable for use with HaloTag fusion proteins expressed in E. coli, as well as HaloTag fusion proteins expressed in mammalian and cell-free systems