missing translation for 'onlineSavingsMsg'
Learn More
Please login to your online account to display your discounted pricing

Thermo Scientific™ iodoTMTsixplex™ Isobaric Mass Tagging Kit

Perform measurement of protein and peptide cysteine modifications (S-nitrosylation, oxidation and disulfide bridges) by multiplex quantitative mass spectrometry.

Manufacturer:  Thermo Scientific™ 0090103

Catalog No. PI90103



Description

Description

Perform measurement of protein and peptide cysteine modifications (S-nitrosylation, oxidation and disulfide bridges) by multiplex quantitative mass spectrometry with Thermo Scientific™ Pierce Cysteine-Reactive Tandem Mass Tag Reagents.


Additional Information and Online Ordering »

Tandem Mass Tag™ (TMT™) Reagents enable concurrent identification and multiplexed quantitation of proteins in different samples using tandem mass spectrometry. The iodoTMT™ Reagents are sets of isobaric (mass and structure) isomers that are iodoacetyl-activated for covalent, irreversible labeling of sulfhydryl (-SH) groups. Similar to iodoacetamide, iodoTMT Reagents react specifically with reduced cysteines (Cys) in peptides and proteins. IodoTMT Reagents can be differentiated by mass spectrometry (MS), enabling quantitation of the relative abundance of cysteine modifications, such as S–nitrosylation, oxidation and disulfide bonds, in cultured cells grown or treated with different conditions.

Highlights:

  • Specific – only reacts with sulfhydryl groups (reduced, unmodified cysteine residues)

  • Irreversible – labeled proteins and peptides are not susceptible to reducing agents

  • Flexible – options for duplex isotopic (MS) or sixplex isobaric (MS/MS) quantitation

  • Versatile – can be used to study cysteine modifications (e.g. di-sulfide bridges, S-nitrosylation)

Note:

IodoTMT Products replace our previously offered CysTMT Reagents, which utilized a dithiopyridine reactive group to reversibly label cysteine sulfhydryls.

Specifications

Specifications

• iodoTMT6-126 Label Reagent, 5 x 0.2 mg
• iodoTMT6-127 Label Reagent, 5 x 0.2 mg
• iodoTMT6-128 Label Reagent, 5 x 0.2 mg
• iodoTMT6-129 Label Reagent, 5 x 0.2 mg
• iodoTMT6-130 Label Reagent, 5 x 0.2 mg
• iodoTMT6-131 Label Reagent, 5 x 0.2 mg
• HES Buffer, 15 mL
• Bond-Breaker TCEP Solution, 0.5 mL
• DTT, No-Weigh Format, 8 x 7.7 mg
• Trypsin, 5 x 20 µg
• Trypsin Storage Solution, 250 µL
• Albumin (bovine), 2.5 mg

Upon receipt store all products at -20°C
Mass Spectrometry
Cell Lysate
iodoTMTsixplex Isobaric Mass Tag Labeling Kit
iodoTMTsixplex™
Tandem Mass Tag™ (TMT) Labels
Protein
Protein Labeling
Mass Spectrometer
SDS
Documents
Product Certifications
Promotions

Promotions