Thermo Scientific™ EZ-Link™ Phosphine-PEG3-Biotin

The phosphine group reacts with an azide to produce an aza-ylide intermediate that is trapped to form a stable, covalent amide bond, which is also referred to as the Staudinger reaction. The azide group, which is the reactive target of phosphine-activated biotin, can be introduced into proteins or other cellular targets via azide reagents or through in vivo labeling with azide-derivatives of naturally occurring metabolic building blocks. Because neither phosphines nor azides are present in biological systems, they comprise chemoselective (mutually specific) ligation pairs for labeling and conjugation. Thus, when used in combination with azide labeling strategies, Phosphine-PEG₃-Biotin enables directed biotin labeling to facilitate detection or affinity purification of protein interactions and post-translational modifications using streptavidin probes or streptavidin agarose resins.

Highlights:

• Soluble easily dissolves in water-miscible solvents (e.g., DMSO) for subsequent dilution in aqueous reaction mixtures with cell lysates and other biological samples

• Compatible reaction chemistry occurs effectively in simple buffer conditions; requires no accessory reagents such as copper or reducing agents

• Chemoselective the phosphine reactive group is specific in biological samples for azide-tagged molecules, ensuring that biotinylation is specific

• PEG spacer polyethylene glycol spacer arm helps maintain solubility of labeled molecules and decreases steric hindrance for affinity-binding to avidin, streptavidin or NeutrAvidin Protein