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Thermo Scientific™ Phusion Buffers

Choose between two buffer packs to optimize high-fidelity PCR or difficult or long template amplification using Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerases.

Manufacturer: thermo scientific™  F520L

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Catalog No. F520L

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Description & Specifications


Storage Requirements Store at -20°C. Stable for one year from the packaging date.
Description Detergent-free Phusion™ HF Buffer Pack
Includes Detergent-free 5x Phusion HF Buffer, 50 mM MgCl2 solution, 100% DMSO.
Quantity 3mL
For Use With (Application) DHPLC and microarray applications

Two optimized buffers are available for Thermo Scientific Phusion High-Fidelity DNA Polymerases: 5X Phusion HF Buffer and 5X Phusion GC Buffer. The error rate of Phusion DNA Polymerase in HF Buffer (4.4 × 10-7) is lower than that in GC Buffer (9.5 × 10-7). Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures. For applications such as microarray or DHPLC, where the DNA templates need to be free of detergents, we recommend using detergent-free reaction buffers.

5X Phusion Green Buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffers do not interfere with the enzyme performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Detergent-free HF Buffer for Phusion DNA Polymerases Developed for DHPLC and microarray applications


Detergent-free 5x Phusion HF Buffer, 50 mM MgCl2 solution, 100% DMSO.