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Thermo Scientific™ Phusion™ HF and GC Buffer Packs

Catalog No. p-200085139
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Chemical Name or Material:
Detergent-free FF Buffer Pack
Detergent-free GC Buffer Pack
GC Buffer Pack
HF Buffer Pack
Color:
Colorless
Green
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Catalog No. Chemical Name or Material Color
F518L HF Buffer Pack Colorless
F519L GC Buffer Pack Colorless
F520L Detergent-free FF Buffer Pack Colorless
F521L Detergent-free GC Buffer Pack Colorless
F539L GC Buffer Pack Green
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Catalog No. F518L Supplier Thermo Scientific™ Supplier No. F518L
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Description

5X Phusion Green buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel.

The error rate of Phusion DNA Polymerase in HF Buffer (4.4 x 10-7) is lower than that in GC Buffer (9.5 x 10-7). Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures. For applications such as microarray or DHPLC, where the DNA templates need to be free of detergents, we recommend using detergent-free reaction buffers.

5X Phusion Green Buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffers do not interfere with the enzyme performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Two optimized buffers are available for Thermo Scientific Phusion High-Fidelity DNA Polymerases:

  • 5X Phusion HF Buffer (F-518L, F-538L (Phusion Green), F-520L (Detergent-free))
  • 5X Phusion GC Buffer (F-519L, F-539L (Phusion Green), F-521L (Detergent-free))

Specifications

Chemical Name or Material HF Buffer Pack
Color Colorless
Description Phusion™ HF Buffer Pack
For Use With (Application) Standard PCR
Quantity 4 x 1.5 mL
Includes • 5X Phusion HF Reaction Buffer (4 x 1.5 mL)
• 50 mM MgCl2 (4 x 1.5 mL)
• DMSO (0.5 mL)

Store at –20°C.

For Research Use Only. Not for use in diagnostic procedures.