Disengage threads and dismount spigot before autoclaving
Follow autoclave instructions provided with the product for proper function
Note: Tubing adapter is not autoclavable
Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement. †Nalgene assigns the term “leakproof”: a) To bottle/closure systems with closures smaller than 100mm, after they are filled with water, inverted, withstand air pressure of 2psig for two minutes, and no water escapes; b) To bottle/closure systems of 100 or 120mm after they are filled with water, inverted for 15 minutes, and no water escapes. Note: These tests, using other liquids, may not yield the same results. To ensure safe usage, Nalgene advises customers to test bottles and closures under conditions of their planned applications. Nalgene bottles and carboys are leakproof at ambient temperature and pressure when used with their Nalgene closures.