MES, is often used as a running buffer for resolving very small proteins on bis-tris gels, also used as a biological buffer in plant cell cultures. It is also been used in various protein characterization assays. This sterile solution suitable for cell culture and molecular biology applications. It is also used as a buffering agent in biology and biochemistry.
Soluble in water (0.5 M at 20°C).
Solutions are stable at 2-8°C for months. Sterilize by filtration through 0.2 mm filters. Autoclaving is not recommended for any sulfonic acid buffer. If buffers must be nuclease-free, treat the water first, then add the buffer after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably). The identity of the yellow breakdown product is unknown.
|Soluble in water (0.5 M at 20°C).|
|1.0M buffer soln.|
|MES buffer soln.|
Safety and Handling
GHS H Statement
Causes skin irritation.
Causes serious eye irritation.
May cause respiratory irritation.
TSCA : No
Recommended Storage : Ambient temperatures