missing translation for 'onlineSavingsMsg'
Learn More
Please login to your online account to display your discounted pricing
  1. Home
  2. Products
  3. PCR Equipment and Supplies
  4. PCR Supplies
  5. Reverse Transcription Products
  6. cDNA Synthesis Kits and Master Mixes
  7. 18080051

Invitrogen™ SuperScript™ III First-Strand Synthesis System DFS Item

Optimized to synthesize first-strand cDNA from purified poly(A)+ or total RNA or RT-PCR

Supplier:  Invitrogen™ 18080051

Catalog No. 18080051



Includes: Oligo(dT)20 (50μM), 50μL; Random hexamers (50ng/μL), 250μL; 10X RT buffer, 1mL; 0.1M DTT, 250μL; 25mM magnesium chloride, 500μL; 10mM dNTP mix, 250μL; SuperScript III RT (200U/μL), 50μL; RNaseOUT (40U/μL), 100μL; E. coli RNase H (2U/μL), 50μL; DEPC-treated water, 1.2mL; Total HeLa RNA (10ng/μL), 20μL; Sense Control Primer (10μM), 25μL; Antisense Control Primer (10μM), 25μL

Description

Description

RNA targets from 100 bp to >12kb can be detected with this system. The amount of starting material can vary from 1pg to 5μg of total RNA. SuperScript III Reverse Transcriptase is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability. The enzyme is used to synthesize cDNA at a temperature range of 42° to 55°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. Because SuperScript III RT is not significantly inhibited by ribosomal and transfer RNA, it may be used to synthesize first-strand cDNA from a total RNA preparation.

  • Using the SuperScript III First-Strand System: cDNA synthesis is performed in the first step using either total RNA or poly(A)+-selected RNA primed with oligo(dT), random primers, or a gene-specific primer
  • In the second step, PCR is performed in a separate tube using primers specific for the gene of interest
  • For the PCR reaction, one of the following DNA polymerases is recommended
  • Platinum Taq DNA Polymerase provides automatic hot-start conditions for increased specificity up to 4kb
  • Platinum Taq DNA Polymerase High Fidelity provides increased yield and high fidelity for targets up to 15kb
  • Platinum Pfx DNA Polymerase provides maximum fidelity for targets up to 12kb

  • Optimal reaction temperature: 50°C
  • Sufficient for 50 reactions, based on a 20μL reaction size

PCR & Real-Time PCR, Reverse Transcription

Order Info

Shipping Condition: Dry ice

Specifications

Specifications

• Oligo(dT)20, 50 μL (50 μM)
• Random hexamers, 250 μL (50 ng/μL)
• 10X RT buffer, 1 mL
• DTT, 250 μL (0.1 M)
• Magnesium chloride, 500 μL (25 mM)
• dNTP mix, 250 μL (10 mM)
• SuperScript III RT, 50 μL (200 U/μL)
• RNaseOUT, 100 μL (40 U/μL)
• E. coli RNase H, 50 μL (2 U/μL)
• DEPC-treated water, 1.2 mL
• Total HeLa RNA, 20 μL (10 ng/μL)
• Sense Control Primer, 25 μL (10 μM)
• Antisense Control Primer, 25 μL (10 μM)

Store at –20°C.
First-Strand Synthesis System
50 Reactions
Separate components
Reverse Transcription
Reverse Transcription
50°C
Dry Ice
First-Strand cDNA
High
RT-PCR, RT-qPCR
50 min.
RNA
20 μL
SuperScript III
Microarray Analysis, Reverse Transcriptase PCR (RT-PCR)
SDS
Documents

Documents

Product Certifications
Promotions

Promotions

For Research Use Only. Not for use in diagnostic procedures.