Invitrogen™ SuperScript™ II Reverse Transcriptase 

Description

SuperScript II Reverse Transcriptase (RT) is a DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or from an RNA:DNA hybrid. This enzyme is genetically engineered by the introduction of point mutations in the RNase H active center to reduce RNase H activity. This structural modification eliminates degradation of RNA molecules during first-strand cDNA synthesis and gives SuperScript II RT superior performance characteristics.

• Yield: Reduced RNase H activity results in greater first-strand cDNA yields
• Size of cDNA: Generation of RT-PCR products up to 12kb
• Thermostability:Full activity at 42°C
• Flexibility:Uses either total or poly(A)+ RNA
• Performance and quality testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product
• Concentration: 200 U/μL
• Unit definition:One unit of SuperScript II RT is the amount of enzyme required to incorporate 1nmole of deoxyribonucleotide into acid-precipitable material in 10 minutes at 37°C using poly(A)•oligo(dT)_12-18 as template
• primer Unit reaction conditions: 50mM Tris-HCl (pH 8.3), 40mM KCl, 6mM MgCl₂, 1mM DTT, 0.5mM [³H]dTTP, 0.1mM poly(A), 0.1mM oligo(dT)_12-18, 0.1mg/mL BSA, and enzyme in 50μL for 10 minutes at 37°C

• Optimal reaction temperature: 42°C
• Final product size: 12.3kb or less

PCR & Real-Time PCR, Reverse Transcription