Please login to your online account to display your discounted pricing

Invitrogen™ SuperScript™ II Reverse Transcriptase DFS Item

An improved version of SuperScript RT

$144.90 - $1,226.40

Specifications

Packaging Type Tube
View More Specs

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Products
Catalog Number Mfr. No. Includes Quantity Price Quantity    

18064022

DFS Item
 
life technologies
18064022
SuperScript II Reverse Transcriptase (2,000 units total, at 200U/μL), 5X first-strand buffer [250mM Tris-HCl (pH 8.3), 375mM KCl, 15mM MgCl2], and 100mM DTT 10 reactions (2,000 units) Each for $144.90

18064071

DFS Item
 
life technologies
18064071
SuperScript II Reverse Transcriptase (4 × 10,000 units total, at 200U/μL), 5X first-strand buffer [250mM Tris-HCl (pH 8.3), 375mM KCl, 15mM MgCl2], and 100mM DTT 200 reactions Each for $1,226.40

18064014

DFS Item
 
life technologies
18064014
SuperScript II Reverse Transcriptase (10,000 units total, at 200U/μL), 5X first-strand buffer [250mM Tris-HCl (pH 8.3), 375mM KCl, 15mM MgCl2], and 100mM DTT 50 reactions (10,000 units) Each for $333.90
Description & Specifications

Specifications

Packaging Type Tube

SuperScript II Reverse Transcriptase (RT) is a DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or from an RNA:DNA hybrid. This enzyme is genetically engineered by the introduction of point mutations in the RNase H active center to reduce RNase H activity. This structural modification eliminates degradation of RNA molecules during first-strand cDNA synthesis and gives SuperScript II RT superior performance characteristics.

  • Yield: Reduced RNase H activity results in greater first-strand cDNA yields
  • Size of cDNA: Generation of RT-PCR products up to 12kb
  • Thermostability:Full activity at 42°C
  • Flexibility:Uses either total or poly(A)+ RNA
  • Performance and quality testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product
  • Concentration: 200 U/μL
  • Unit definition:One unit of SuperScript II RT is the amount of enzyme required to incorporate 1nmole of deoxyribonucleotide into acid-precipitable material in 10 minutes at 37°C using poly(A)•oligo(dT)12-18 as template
  • primer Unit reaction conditions: 50mM Tris-HCl (pH 8.3), 40mM KCl, 6mM MgCl2, 1mM DTT, 0.5mM [3H]dTTP, 0.1mM poly(A), 0.1mM oligo(dT)12-18, 0.1mg/mL BSA, and enzyme in 50μL for 10 minutes at 37°C

  • Optimal reaction temperature: 42°C
  • Final product size: 12.3kb or less

PCR & Real-Time PCR, Reverse Transcription

Shipping Condition: Wet ice