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Invitrogen™ SuperScript™ II Reverse Transcriptase DFS Item

An improved version of SuperScript RT

$160.00 - $2,040.00

Specifications

Concentration 200 U/μL
Final Product Type First-Strand cDNA
Format Reverse Transcriptase
GC-Rich PCR Performance High
PCR Method RT-PCR, RT-qPCR
View More Specs

Products 3
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
18064022 DFS Item
View Documents Promotion Details
Invitrogen™
18064022
2,000 Units
Each for $160.00
 
18064071 DFS Item
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Invitrogen™
18064071
Each for $2,040.00
 
18064014 DFS Item
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Invitrogen™
18064014
10,000 Units
Each for $592.00
 
Description

Description

SuperScript II Reverse Transcriptase (RT) is a DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or from an RNA:DNA hybrid. This enzyme is genetically engineered by the introduction of point mutations in the RNase H active center to reduce RNase H activity. This structural modification eliminates degradation of RNA molecules during first-strand cDNA synthesis and gives SuperScript II RT superior performance characteristics.
  • Yield: Reduced RNase H activity results in greater first-strand cDNA yields
  • Size of cDNA: Generation of RT-PCR products up to 12kb
  • Thermostability:Full activity at 42°C
  • Flexibility:Uses either total or poly(A)+ RNA
  • Performance and quality testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product
  • Concentration: 200 U/μL
  • Unit definition:One unit of SuperScript II RT is the amount of enzyme required to incorporate 1nmole of deoxyribonucleotide into acid-precipitable material in 10 minutes at 37°C using poly(A)•oligo(dT)12-18 as template
  • primer Unit reaction conditions: 50mM Tris-HCl (pH 8.3), 40mM KCl, 6mM MgCl2, 1mM DTT, 0.5mM [3H]dTTP, 0.1mM poly(A), 0.1mM oligo(dT)12-18, 0.1mg/mL BSA, and enzyme in 50μL for 10 minutes at 37°C
  • Optimal reaction temperature: 42°C
  • Final product size: 12.3kb or less

PCR & Real-Time PCR, Reverse Transcription

Order Info

Shipping Condition: Wet ice

Specifications

Specifications

200 U/μL
Reverse Transcriptase
RT-PCR, RT-qPCR
50 min.
Up to 12.3 kb
Reverse Transcription
SuperScript II
First-Strand cDNA
High
Separate components
Reverse Transcription
RNA
42°C
Reduced
SDS
Product Certifications

For Research Use Only. Not for use in diagnostic procedures.