Invitrogen™ PureLink™ HiPure Plasmid Miniprep Kit 

Description

The PureLink HiPure Plasmid Miniprep Kit allows isolation of high yields of highly pure plasmid DNA from E.coli in less than 2 hours, without use of any organic solvents or cesium chloride, and contains Low endotoxin levels.

Plasmid DNA of High Purity

• PureLink HiPure Plasmid Miniprep Kit is designed to efficiently isolate plasmid DNA from E.coli in 1.5–2 hours
• Kit employs anion-exchange resin composed of small particles with a uniform pore size to purify plasmid DNA to a level equivalent to two passes through CsCl gradients
• Need for additional steps to remove contaminants such as phenol, chloroform, and CsCl are eliminated, minimizing exposure to and disposal of hazardous materials
• Efficient—Increased yields with low endotoxin Levels

High Yield and Efficient Endotoxin Removal

• PureLink HiPure Plasmid Miniprep Kit allows purification of up to 30 μg of high quality plasmid DNA from 1–3 mL overnight E.coli cultures in approximately 1 hour, with an endotoxin level of 0.1–1 EU/μg, when cloning high copy number plasmids
• Typically, DNA isolated using the PureLink HiPure Plasmid Miniprep Kit has an A₂₆₀/A₂₈₀ >1.80 when samples are diluted in Tris-HCl (pH 7.5) indicating that the DNA is reasonably free of proteins that could interfere with downstream applications

Isolated DNA can be used for a Number of Downstream Applications:

• Purified DNA is suitable for downstream applications, including those requiring the highest purity DNA, such as mammalian transfections, automated and manual DNA sequencing, PCR amplification, in vitro transcription, bacterial cell transformation, cloning, and labeling