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Invitrogen™ Dynabeads™ MyOne™ Streptavidin C1 DFS Item

Dynabeads MyOne Streptavidin C1 beads are 1-μm monosized, monodispersed, superparamagnetic beads covalently coupled to streptavidin.

$850.00 - $2895.00

Specifications

Binding Property >2,500 pmoles free biotin/mg beads
Certifications/Compliance ISO9001 and ISO13485
Concentration 10 mg/mL
Content And Storage Dynabeads MyOne Streptavidin C1 are supplied in PBS, pH 7.4, with 0.01% Tween-20 and 0.09% NaN3. Store at 2–8°C.

Description Recombinant streptavidin covalently coupled to Dynabeads
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Includes: Dynabeads™ are supplied in PBS, pH 7.4 with 0.01% Tween™-20 and 0.02% NaN3 added as preservatives.

Products 2
Catalog Number Mfr. No. Volume (Metric) Price Quantity  
Catalog Number Mfr. No. Volume (Metric) Price Quantity  
65001 DFS Item
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LS65001
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65002 DFS Item
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Invitrogen™
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Description

Description

Dynabeads MyOne Streptavidin C1 beads are 1-μm monosized, monodispersed, superparamagnetic beads covalently coupled to streptavidin. The product is intended for use in the isolation and handling of biotinylated nucleic acids, antibodies, and other biotinylated ligands. Their small but uniform size presents a high surface area per mg of beads and a correspondingly high capacity for the target ligand, while the high iron content ensures an efficient magnetic pull. These features, along with a slow sedimentation rate during incubation contribute to a gentle and rapid magnetic separation. The excellent dispersion abilities and the absence of magnetic remanence make the Dynabeads streptavidin products ideal for both manual and automated protocols, including microfluidic system protocols.

Benefits of Dynabeads MyOne Streptavidin C1 beads include:
Performance—high sensitivity and reproducibility secure consistent results
Efficiency-—ast binding kinetics and high binding capacity
Flexibility—flexible protocols with gentle and efficient liquid-phase reaction kinetics
Automation ready—easy automation for high-throughput needs on the Kingfisher Purification System

Consistency and reproducibility
The monolayer of streptavidin leaves most biotin-binding sites available for the binding of free biotin and biotinylated ligands. The specific and defined surface allows for efficient capture, separation, and downstream handling. In addition, the low size distribution (<5%) of the monosized beads and the streptavidin monolayer ensure negligible leakage, providing batch consistency and reproducibility of results.

High binding capacity
The size of the ligand and the biotinylation procedure will affect the binding capacity. The capacity also depends on steric availability and charge interaction between bead and ligand and between ligands. With two or three biotin binding sites available for each streptavidin molecule on the surface of the bead after immobilization, one mg of Dynabeads MyOne Streptavidin C1 typically binds:
• >2,800 pmoles free biotin
• ∼20 μg biotinylated IgG 
• ∼400 pmol biotinylated peptides 
• ∼20 μg ds-DNA 
• ∼500 pmol ss-oligonucleotides

Applications 
Thermo Fisher streptavidin-coupled Dynabeads magnetic beads have been used and cited for a wide variety of applications. Although the beads can be used to isolate any biotinylated targets, these hydrophilic beads are particularly well suited for the preparation of ssDNA, high-throughput nucleic acid clean-up protocols, and protein purifications prior to mass spectrometry.

Automation ready with the KingFisher Purification System
The 1-μm magnetic beads are ideal for high-throughput enrichment. This process can be automated using any of our KingFisher Sample Purification Systems or liquid handlers.

Commercial supply 
Our manufacturing sites are ISO 13485-certified. Bead characteristics and manufacturing conditions help ensure consistency and batch reproducibility making them an ideal choice for commercial supply. If you are in the process of customizing Dynabeads MyOne Streptavidin C1 beads in a commercial product or service or need a larger bulk volume of this product, please contact us at oemdynal@lifetech.com or see our Dynabeads OEM page.

Order Info

Shipping Condition: Room Temperature

Specifications

Specifications

>2,500 pmoles free biotin/mg beads
10 mg/mL
Recombinant streptavidin covalently coupled to Dynabeads
Capture of Biotinylated Targets
Streptavidin Magnetic Beads
KingFisher™ Sample Purification System, DynaMag™ magnets
Magnetic Beads
0.09% NaN3
Ambient Temperature
Carboxylic Acid, Hydrophilic
For Research Use Only
ISO9001 and ISO13485
Dynabeads MyOne Streptavidin C1 are supplied in PBS, pH 7.4, with 0.01% Tween-20 and 0.09% NaN3. Store at 2–8°C.

Brown
Magnetic Polystyrene
Beads in suspension
Yes
Streptavidin
Any Sample Type
CV <5%
1 μm
SDS
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For Research Use Only. Not for use in diagnostic procedures.