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Applied Biosystems™ Power SYBR™ Green RNA-to-CT™ 1-Step Kit 
The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.
Supplier: Applied Biosystems™ 4391178
Description
The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.
- Get sensitivity and specificity in an easy-to-use one-step qRT-PCR reaction.
- Formulated to ensure maximum sensitivity and reliability
- Reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets
Includes:
- AmpliTaq™ Gold DNA Polymerase UP for hot start qPCR, minimizing non-specific product formation
- ArrayScript™ UP Reverse Transcriptase, an engineered RT that produces high yields of full-length cDNA
- An additive that reduces primer dimer formation, reducing non-specific products that can cause false positive signals
- RNase inhibitor, to prevent degradation of RNA templates
- A proprietary ROX™ dye that serves as a passive internal reference to normalize non-PCR-related fluorescence fluctuations for superb precision on Applied Biosystems™ real-time PCR instruments
Specifications
| Sufficient for 40 reactions at 50 μL reaction volume and includes: • 1 x 1 mL tube of 2X master mix containing SYBR™Green 1 Dye, AmpliTaq Gold™ DNA Polymerase UP, dNTPs, Passive Reference 1, and optimized buffer components. Store at 2-8°C after opening. • 1 x 20 μL tube of 125X RT enzyme mix containing ArrayScript™ UP Reverse Transcriptase and RNase Inhibitor. Store at -15 to -30°C. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA). |
|
| Tube | |
| AmpliTaq Gold DNA Polymerase | |
| 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System | |
| SYBR Green | |
| RNA-to-CT™, SYBR™ | |
| UP (Ultra Pure) | |
| ArrayScript™ UP | |
| Wet Ice | |
| Gene Expression | |
| 40 Reactions | |
| 1 x 1 mL |
| SYBR | |
| 1-step RT-qPCR | |
| Standard | |
| Green RNA-to-CT 1-Step Kit | |
| ROX (Pre-mixed) | |
| Real Time PCR SYBR Master Mix | |
| 1 x 1 mL | |
| Standard | |
| 40 Reactions at 50 μL | |
| 2X (MasterMix), 125X (RT Enzyme Mix) | |
| RNA |
For Research Use Only. Not for use in diagnostic procedures.