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Cytiva HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

$71.58 - $918.10

Specifications

Material Polypropylene
pH Range 3 to 12
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
Support Type 6% Highly Cross-linked Agarose
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Products 4
Catalog Number Mfr. No. Diameter (Metric) Inner Quantity Flow Rate Price Quantity  
Catalog Number Mfr. No. Diameter (Metric) Inner Quantity Flow Rate Price Quantity  
45-000-323
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Cytiva
17524701
7 mm 5 x 1 mL 4 mL/min. (1 mL), 5 mL/min. (5 mL)
Pack of 5 Each for $276.55
 
45-000-324
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Cytiva
17524801
16 mm 1 x 5 mL 4 mL/min. (1 mL), 5 mL/min. (5 mL)
Each for $234.86
 
45-000-325
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Cytiva
17524802
16 mm 5 x 5 mL 4 mL/min. (1 mL), 5 mL/min. (5 mL)
Pack of 5 Each for $918.10
 
45-000-513
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Cytiva
29051021
7 mm 1 mL 4 mL/min. (1 mL), 5 mL/min. (5 mL)
Each for $71.58
 
Description

Description

  • Prepacked with Ni Sepharose™ High Performance medium
  • Negligible nickel ion leakage
  • Pressure Max: 3 bar
  • Column material: polypropylene
  • 1 and 5mL columns
  • Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
  • Manual operation with syringe, pump or chromatography system

Media
  • Matrix: 6% cross-linked agarose
  • Average particle size: 34μm
  • pH stability working range: 3 to 12

1mL columns

  • Bed Dimensions: 7 x 25mm
  • Flow velocity: 4mL/min.

5mL columns
  • Bed Dimensions: 16 x 25mm
  • Flow velocity: 5mL/min.

Specifications

Specifications

Polypropylene
3 to 12
5 bar (0.5 MPa, 70 psi)
6% Highly Cross-linked Agarose
SDS
Product Certifications