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Cytiva HisTrap™ HP Prepacked Columns
Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
$71.58 - $918.10
Specifications
| Material | Polypropylene |
|---|---|
| pH Range | 3 to 12 |
| Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
| Support Type | 6% Highly Cross-linked Agarose |
| Catalog Number | Mfr. No. | Diameter (Metric) Inner | Quantity | Flow Rate | Price | Quantity | |||
|---|---|---|---|---|---|---|---|---|---|
| Catalog Number | Mfr. No. | Diameter (Metric) Inner | Quantity | Flow Rate | Price | Quantity | |||
|
45-000-323
|
Cytiva
17524701 |
7 mm | 5 x 1 mL | 4 mL/min. (1 mL), 5 mL/min. (5 mL) |
Pack of 5 Each for $276.55
|
|
45-000-324
|
Cytiva
17524801 |
16 mm | 1 x 5 mL | 4 mL/min. (1 mL), 5 mL/min. (5 mL) |
Each for $234.86
|
|
45-000-325
|
Cytiva
17524802 |
16 mm | 5 x 5 mL | 4 mL/min. (1 mL), 5 mL/min. (5 mL) |
Pack of 5 Each for $918.10
|
|
45-000-513
|
Cytiva
29051021 |
7 mm | 1 mL | 4 mL/min. (1 mL), 5 mL/min. (5 mL) |
Each for $71.58
|
|
Description
- Prepacked with Ni Sepharose™ High Performance medium
- Negligible nickel ion leakage
- Pressure Max: 3 bar
- Column material: polypropylene
- 1 and 5mL columns
- Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
- Manual operation with syringe, pump or chromatography system
Media
- Matrix: 6% cross-linked agarose
- Average particle size: 34μm
- pH stability working range: 3 to 12
1mL columns
- Bed Dimensions: 7 x 25mm
- Flow velocity: 4mL/min.
5mL columns
- Bed Dimensions: 16 x 25mm
- Flow velocity: 5mL/min.
Specifications
| Polypropylene | |
| 3 to 12 | |
| 5 bar (0.5 MPa, 70 psi) | |
| 6% Highly Cross-linked Agarose |