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Gibco™ Expi293™ Inducible GnTI- Expression System Kit DFS Item

Expi293 Inducible GnTI- Expression System Kit is part of the Expi293 platform for rapid, high-yield transient protein expression from mammalian 293 cells

Manufacturer:  Gibco™ LSA39252

Catalog No. A39252



Description

Description

  • System kit is centered on engineered Expi293F cells that do not have N-acetylglucosaminyltransferase I (GnTI) activity, therefore lacking complex N-glycans, and stably express the tetracycline repressor (TetR) protein, thus enabling tetracycline-regulated protein expression
  • Expi293F Inducible GnTI- cell line was established by the selective knockout of the GnTI gene and the stable integration of the regulatory plasmid pcDNA6/TR, which encodes the TetR gene under the control of the human CMV promoter, in parental Expi293F cells
  • This cell line is a powerful tool for tetracycline-inducible high-yield expression of homogeneously glycosylated recombinant proteins.

Features:

  • Homogeneous N-glycosylation of expressed proteins
  • Tetracycline-inducible protein expression with extremely low basal expression levels
  • Equivalent protein yields to parental Expi293F cells—up to 1 g/L
  • pcDNA5/TO expression vector for tetracycline-regulated expression of the gene of interest
  • Suspension, high-density culture of Expi293F Inducible GnTI- cells in Expi293 Expression Medium
  • High-efficiency transient transfection using ExpiFectamine 293 reagent in combination with specialized transfection enhancers

Components included:

  • 1 vial of frozen Expi293F Inducible GnTI- cells
  • 1 L of Expi293 Expression Medium
  • 1 ExpiFectamine 293 transfection kit sufficient to transfect 1 L of culture,
  • 100 mL Opti-Plex Complexation Buffer
  • 1  PNGase F Glycan Cleavage kit
  • 1 pcDNA5/TO Mammalian Expression Vector kit
  • 500 mg tetracycline hydrochloride
  • pRABBIT IgG IRES-EmGFP positive control vector

All components of the Expi293 Inducible GnTI- Expression System are also available for individual purchase

Specifications

Specifications

Expi293F™
Transfection
Expi293™
Approved for shipment at Room Temperature or on Wet or Dry Ice
• Expi293F Inducible GnTI- Cells (1x107 cells/vial), store in vapor-phase liquid nitrogen
• 1 L Expi293 Expression Medium, store at 2–8°C (protect from light)
• ExpiFectamine 293 Transfection Kit for 1 L of culture, store at 2–8°C
• PNGase F Glycan Cleavage Kit, store at -20C
• 100 mL Opti-Plex Complexation Buffer, store at 2–8°C (protect from light)
• 20 µg pcDNA5/TO Mammalian Expression Vector, store at 2–8°C or long term storage at -20°C.
• 500 mg Tetracycline Hydrochloride, store at -20°C
• pRABBIT IgG IRES-EmGFP Positive Control Vector, store at 2–8°C or long term storage at -20°C

Protein Production
CMV⁄TO
SDS
Documents

For Research Use Only. Not for use in diagnostic procedures.