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Applied Biosystems™ SYBR™ Select Master Mix for CFX DFS Item

A cost-effective solution for real-time PCR applications.

Manufacturer:  Applied Biosystems™ 4472954

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Catalog No. 4472954



  • Specific - minimize primer-dimer and non-specific amplification
  • Reproducible and sensitive - consistent amplification across a wide dynamic range
  • Bright - contains SYBR™ GreenER™ dye for maximum brightness
  • Carry-over contamination control - contains heat-labile UDG
  • For use with the Bio-Rad CFX96™ Touch or CFX384™ Touch systems
  • Standard and fast cycling parameters

Formulated for Maximum Specificity:
  • SYBR™ Select Master Mix for CFX contains all the components needed for your real-time PCR reactions, except the template and primers, in a convenient 2X concentration premix
  • The master mix includes AmpliTaq™ DNA Polymerase, UP, a highly purified DNA polymerase with a proprietary hot start mechanism that provides exceptional specificity

Obtain Reproducible Results Across a Wide Dynamic Range:
  • SYBR™ Select Master Mix for CFX is specially formulated to provide robust results from 100ng to 0.1pg cDNA per reaction and can reliably detect a single copy of genomic DNA.
    • Contains SYBR™ GreenER™ Dye and Heat-labile UDG:
    • SYBR™ GreenER™ dye is less inhibitory to PCR than SYBR™ Green I dye, resulting in brighter signals
    • Heat-labile uracil-DNA glycosylase (UDG) is included for worry-free carryover contamination control

    Order Info

    Shipping Conditions: Wet Ice



The 2x mix contains SYBR™ GreenER™ dye, AmpliTaq™ DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, and optimized buffer components. Contains 10 X 5 mL bottles, sufficient for 5000 reactions at 20 µL. Store at 2-8™C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Built-In Hot Start
UP (Ultra Pure)
DNA (Genomic), cDNA
Real Time PCR (qPCR)
SYBR™ GreenER™
Unlabeled Oligos
Real Time PCR SYBR Master Mix
5000 Reactions of 20 μL
5000 Reactions
AmpliTaq™ DNA Polymerase
Fast, Standard
10 x 5 mL
AB 7900HT, Standard Mode, AB StepOne™, Fast Mode, AB 7500, Standard Mode, AB 7500, Fast Mode, Bio-Rad CFX96 Touch™, AB StepOnePlus™, Fast Mode, AB 7900HT, Fast Mode, AB StepOne™, Standard Mode, Bio-Rad CFX Connect™, AB 7900 HT 384-Well System, Bio-Rad CFX384 Touch™, AB StepOnePlus™, Standard Mode
ROX (Pre-mixed)
10 x 5 mL
Product Certifications


For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.