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Invitrogen™ RNAqueous™ Total RNA Isolation Kit 
Uses glass fiber filter separation technology
Supplier: Invitrogen™ AM1912
Description
The RNAqueous Kit is for phenol-free total RNA isolation from 0.1g to 0.5g of tissue or from 107 to 108 cells, using a guanidinium-based lysis/denaturant and glass fiber filter separation technology.
- Convenient syringe-based filtration
- Compatibility with vacuum manifolds when processing multiple samples
How it works
The RNAqueous Kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through the filter using a standard syringe (not included). Vacuum filtration is not required; however, the filter units are compatible with standard vacuum manifolds for processing several samples at once.
Not high-throughput compatible (Manual)
Applications:
DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Animal Cells and Tissues
Specifications
| • 6 mL Formaldehyde Load Dye; store at -20°C • 60 mL Lysis/Binding Solution; 4°C • 60 mL Water; 4°C • 4 mL Lithium Chloride Precipitation Solution; 4°C • 40 mL Wash Solution #1; 4°C • 80 mL Wash Solution #2/3 (concentrated); 4°C • 50 Filter Cartridges; room temperature • 100 Collection Tubes; room temperature • 10 mL Elution Solution; room temperature |
|
| 50–120 μL | |
| Total RNA | |
| RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling | |
| Room Temperature |
| Spin Column (Glass Fiber Filter) | |
| Bacteria, Cells, Plant, Tissue, Yeast, Viruses, Enzymatic Reactions | |
| Not High-throughput Compatible (Manual) | |
| 50 Preps | |
| Bacteria: ≤109 cells Cells: ≤107 Tissue: ≤75 mg Plant: ≤75 mg Yeast: ≤108 cells Virus: varies Enzymatic Reactions: varies |