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  5. Invitrogen™ RNAqueous™ Total RNA Isolation Kit

Invitrogen™ RNAqueous™ Total RNA Isolation Kit

Numéro de catalogue. p-200109803
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Quantity:
50 Catridges
50 Preps
Product Type:
RNA Isolation Kit
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Numéro de catalogue. Quantity Product Type
AM1912 50 Preps RNA Isolation Kit
AM10051G 50 Catridges Spin Columns
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Numéro de catalogue. AM1912 Fournisseur Invitrogen™ Code fournisseur AM1912
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Description

The RNAqueous Total RNA Isolation Kit is for phenol-free total RNA isolation from 1–75 mg of tissue or from 10^2–10^7 cells, using a guanidinium-based lysis/denaturant and glass fiber filter separation technology.

The RNAqueous Total RNA Isolation Kit is for phenol-free total RNA isolation from 1–75 mg of tissue or from 102–107 cells, using a guanidinium-based lysis/denaturant and glass fiber filter separation technology.

The RNAqueous kit provides:

  • Convenient syringe-based filtration
  • Compatibility with vacuum manifolds when processing multiple samples

How it works

The RNAqueous kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through the filter using a standard syringe (not included). Vacuum filtration is not required; however, the filter units are compatible with standard vacuum manifolds for processing several samples at once.

Spécifications

Content And Storage • 6 mL Formaldehyde Load Dye; store at -20°C
• 60 mL Lysis/Binding Solution; 4°C
• 60 mL Water; 4°C
• 4 mL Lithium Chloride Precipitation Solution; 4°C
• 40 mL Wash Solution #1; 4°C
• 80 mL Wash Solution #2/3 (concentrated); 4°C
• 50 Filter Cartridges; room temperature
• 100 Collection Tubes; room temperature
• 10 mL Elution Solution; room temperature
Isolation Technology Spin Column (Glass Fiber Filter)
Elution Volume 50–120 μL
Sample Type Bacteria, Cells, Plant, Tissue, Yeast, Viruses, Enzymatic Reactions
Final Product Type Total RNA
High-throughput Compatibility Not High-throughput Compatible (Manual)
For Use With (Application) RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Product Type RNA Isolation Kit
Quantity 50 Preps
Shipping Condition Room Temperature
Starting Material Amount Bacteria: ≤109 cells, Cells: ≤107, Tissue: ≤75 mg, Plant: ≤75 mg, Yeast: ≤108 cells, Virus: varies, Enzymatic Reactions: varies
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For Research Use Only. Not for use in diagnostic procedures.