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Real Time PCR Master Mixes

Real-time PCR master mixes are batch mixtures of PCR reagents at optimal concentrations that can be prepared and divided among multiple PCR tubes/plates. They reduce pipetting and risk of contamination, save time, and limit possible mixing errors.
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1 – 15 of 75 results
1
Promotions Available

Applied Biosystems™ TaqPath™ DuraPlex™ 1-Step RT-qPCR Master Mix

Applied Biosystems TaqPath DuraPlex 1-Step RT-qPCR Master Mix is a benchtop-stable, single-tube master mix optimized for rapid, sensitive, and reproducible detection of viral and bacterial pathogens even in the presence of PCR inhibitors.

Shipping Condition Dry Ice
GC-Rich PCR Performance Medium
Content And Storage Store at -15°C to -25°C.
Store at room temperature (24°C) for 1 week after first use and 4°C for 1 month after first use.
Form Liquid
InhibitorToleranceSimple High
Concentration 4X
For Use With (Application) Microbial Detection,Gene Expression,DNA Quantitation,Presence/Absence
PCR Method 1-step RT-qPCR
Multiplex Capability Up to 5-plex
Detection Method Primer-probe
Includes dUTP
Thermostability 8 hr., Assembled Reaction
Polymerase Applied Biosystems™ AmpliTaq™ DNA Polymerase with Hot Start
Format Single-Tube
Passive Reference Dye ROX (Pre-mixed)
Reaction Speed Fast or Standard
Target Specificity RNA only, Both RNA and DNA
Hot Start Antibody-based
Shelf Life Two years from date of manufacture, with guaranteed minimum shelf life of ≥1 year upon receipt
Product Line TaqPath
2

Fisherbrand™ SYBR™ Green qPCR Master Mix

Fisherbrand™ SYBR™ Green qPCR Master Mix is a 2X, ready-to-use master mix designed for dye-based, quantitative amplification of DNA (cDNA and gDNA) targets by real-time PCR.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Content And Storage SYBR Green dye, DNA polymerase, with an antibody-mediated hot start, Heat-labile uracil-DNA glycosylase (UDG), ROX dye (passive reference dye), dNTP blend containing dUTP/dTTP, Optimized buffer components; Store at -20°C ± 10°C in the dark
Product Type Master Mix
Form Frozen
Reaction Speed Fast or Standard
Label or Dye SYBR Green,FAM
Concentration 2X
For Use With (Application) Gene Expression,DNA Quantitation,Microbial Detection,Real Time PCR (qPCR)
PCR Method qPCR,2-step RT-qPCR,RT-qPCR
Detection Method SYBR
3

Applied Biosystems™ Absolute Q™ Universal DNA Digital PCR Master Mix (5X)

Absolute Q Universal DNA Digital PCR Master Mix is compatible with a broad range of applications and over 20 million Applied Biosystems predesigned TaqMan Assays, eliminating the need to design custom assays.

4

Applied Biosystems™ TaqPath™ DuraPlex™ 1-Step RT-qPCR Master Mix, no ROX™

Applied Biosystems TaqPath DuraPlex 1-Step RT-qPCR Master Mix is a benchtop-stable, single-tube master mix optimized for rapid, sensitive, and reproducible detection of viral and bacterial pathogens even in the presence of PCR inhibitors.

Shipping Condition Dry Ice
GC-Rich PCR Performance Medium
Content And Storage Store at –15 to –25°C.
Store at room temperature (24°C) for 1 week after first use and 4°C for 1 month after first use.
Form Liquid
InhibitorToleranceSimple High
For Use With (Application) Microbial Detection,Gene Expression,DNA Quantitation,Presence/Absence
PCR Method 1-step RT-qPCR
Multiplex Capability Up to 6-plex
Detection Method Primer-probe
Includes dUTP
Thermostability 8 hr., Assembled Reaction
Polymerase Applied Biosystems™ AmpliTaq™ DNA Polymerase with Hot Start
Format Single-Tube
Passive Reference Dye None
Reaction Speed Fast or Standard
Target Specificity RNA only, Both RNA and DNA
Hot Start Antibody-based
Shelf Life Two years from date of manufacture, with guaranteed minimum shelf life of ≥1 year upon receipt
Product Line TaqPath
5
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Invitrogen™ Platinum™ Quantitative PCR SuperMix-UDG

Is a ready-to-use mixture for high-specificity, real-time quantitative DNA amplification, containing UDG to prevent carryover contamination (1,2).

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Product Type Quantitative PCR SuperMix-UDG
For Use With (Equipment) 7500 System, BioRad iCycler iQ, BioRad iQ5, Stratagene Mx4000, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Cepheid SmartCycler, BioRad MyiQ
Sample Type DNA (Genomic), cDNA
Reaction Speed Standard
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line Platinum
Detection Method Primer-probe
6
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Invitrogen™ Platinum™ Quantitative PCR SuperMix-UDG w/ROX

qPCR SuperMixes with ROX for ABI instruments are ready-to-use reaction mixes for high-specificity, real-time amplification of cDNA, genomic DNA or plasmid DNA.

Shipping Condition Dry Ice
Polymerase Platinum Taq DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2 X
Product Type Quantitative PCR SuperMix-UDG
For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT System
Sample Type DNA (Genomic), cDNA
Reaction Speed Standard
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line Platinum
Detection Method Primer-probe
7
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Invitrogen™ EXPRESS qPCR Supermix, universal

EXPRESS qPCR SuperMix kits are compatible with fast-cycling instruments for probe-based qPCR assays.

GC-Rich PCR Performance High
Format Tube
Product Type Express qPCR Supermix
Sample Type DNA (Genomic), cDNA
Reaction Speed Fast or Standard
For Use With (Application) Gene Expression
PCR Method qPCR
Detection Method Primer-probe
8
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Invitrogen™ EXPRESS qPCR Supermix, with premixed ROX

EXPRESS qPCR SuperMix kits are compatible with fast-cycling instruments for your probe-based qPCR assays.

GC-Rich PCR Performance High
Content And Storage EXPRESS qPCR SuperMix kits are provided as a 2X qPCR SuperMix with ROX premixed or in a separate tube.

Store at -20°C upon receipt. Once thawed, the EXPRESS qPCR SuperMix can be stored for up to 1 month at +4·C. Product is guaranteedstable for 6 months when properly stored.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Format Tube
Product Type Express qPCR Supermix
For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT Fast System, 7900HT System, Eppendorf™ MasterCycler™ RealPlex, StepOne™, Fast Mode, StepOne™, Standard Mode
Sample Type DNA (Genomic), cDNA
Reaction Speed Fast or Standard
For Use With (Application) Gene Expression
PCR Method qPCR
Detection Method Primer-probe
9

Applied Biosystems™ TaqMan™ Fast Virus 1-Step Multiplex Master Mix for qPCR (No ROX)

TaqMan Fast Virus 1-Step Multiplex Master Mix (No ROX) is designed to enable dye flexibility for multiplexing up to four different RNA/DNA targets for high-throughput RT-qPCR.

Shipping Condition Dry Ice
InhibitorToleranceSimple High
Concentration 4X
For Use With (Application) Microbial Detection,Gene Expression,DNA Quantitation,Presence/Absence
PCR Method 1-step RT-qPCR
Multiplex Capability Up to 4-plex
Detection Method Primer-probe
Thermostability Not Applicable
Passive Reference Dye None
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 6 Pro, QuantStudio™ 7 Flex, QuantStudio™ 7 Pro, StepOne™, StepOnePlus™, ViiA™ 7 System
Sample Type RNA, DNA
Reaction Speed Fast or Standard
Target Specificity RNA only, Both RNA and DNA
Hot Start Built-In Hot Start
10
Promotions Available

Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR

Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.

Shipping Condition Wet Ice
InhibitorToleranceSimple Medium
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Multiplex Capability Single-plex
Detection Method SYBR
Includes dUTP, UNG/UDG
Thermostability 72 hr., Assembled Reaction
Polymerase Dual-Lock Taq DNA Polymerase
Format Tube
Product Type Real Time PCR SYBR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio 7 Flex, StepOne, StepOnePlus, ViiA 7 System
Sample Type DNA
Reaction Speed Fast or Standard
Target Specificity cDNA or gDNA only
Product Line PowerUP, SYBR
11

Applied Biosystems™ TaqPath™ 1-Step Multiplex Master Mix (No ROX)

TaqPath 1-Step Multiplex Master Mix (No ROX) is a fast, single-tube, 4X RT-qPCR mix that provides for sensitive, reproducible detection of up to four different RNA/DNA targets in a single multiplex reaction.

Shipping Condition Dry Ice
InhibitorToleranceSimple High
For Use With (Application) Microbial Detection,DNA Quantitation,Presence/Absence
PCR Method 1-step RT-qPCR
Multiplex Capability Up to 4-plex
Detection Method Primer-probe
Includes dUTP, UNG/UDG
Thermostability Not Applicable
Sensitivity Down to 10 copies
Format Tube
Passive Reference Dye None
For Use With (Equipment) QuantStudio 6 Pro or QuantStudio 7 Pro, QuantStudio 3 or 5, QuantStudio 6/QuantStudio 7 Flex, QuantStudio 12K Flex, QuantStudio Dx, ViiA 7, 7500, 7500 Fast, 7500 Fast Dx, or use a compatible real-time PCR instrument from another supplier
Sample Type DNA, RNA
Reaction Speed Fast or Standard
Target Specificity RNA only, Both RNA and DNA
Product Line TaqPath
12

Applied Biosystems™ TaqPath™ 1-Step Multiplex Master Mix

TaqPath 1-Step Multiplex Master Mix is a fast, single-tube, 4X RT-qPCR mix that provides for sensitive, reproducible detection of up to four different RNA/DNA targets in a single multiplex reaction.

Shipping Condition Dry Ice
InhibitorToleranceSimple High
Concentration 4X
For Use With (Application) Gene Expression,Microbial Detection
PCR Method 1-step RT-qPCR
Multiplex Capability Up to 4-plex
Detection Method Primer-probe
Includes dUTP, UNG/UDG
Thermostability Not Applicable
Polymerase Fast DNA Polymerase
Sensitivity Down to 10 copies
Format Tube
Product Type qPCR Master Mix
Passive Reference Dye Mustang Purple
For Use With (Equipment) QuantStudio 6 Pro Real-Time PCR System or QuantStudio 7 Pro, QuantStudio 3 or 5, QuantStudio 6/QuantStudio 7 Flex, QuantStudio 12K Flex, QuantStudio Dx, ViiA 7, 7500 , 7500 Fast, 7500 Fast Dx
Sample Type DNA, RNA
Reaction Speed Fast or Standard
Target Specificity RNA only, Both RNA and DNA
Product Line TaqPath
13
Promotions Available

Applied Biosystems™ TaqMan™ Fast Advanced Master Mix for qPCR, no UNG

TaqMan Fast Advanced Master Mix delivers accurate quantification and dependability in a short time.

Shipping Condition Wet Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2 X
Form Liquid
InhibitorToleranceSimple High
Concentration 2X
For Use With (Application) Gene Expression,DNA Quantitation,Presence/Absence
PCR Method qPCR
Multiplex Capability Duplex
Detection Method Primer-probe
Includes dUTP
Thermostability 72 hr., Assembled Reaction
Polymerase AmpliTaq Fast DNA Polymerase
Product Type Fast Advanced Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 6 Pro, QuantStudio™ 7 Flex, QuantStudio™ 7 Pro, StepOne™, StepOnePlus™, ViiA™ 7 System
Sample Type DNA
Reaction Speed Fast or Standard
Target Specificity cDNA or gDNA only
Product Line TaqMan
14
Promotions Available

Applied Biosystems™ PowerUp™ SYBR™ Green & Endura™ 384-well Plates Bundle

Bundle and save up to 50% off PowerUp SYBR Green Master Mix and Plates

15
Promotions Available

Applied Biosystems™ Absolute Q™ DNA Digital PCR Master Mix (5X)

Applied Biosystems Absolute Q DNA Digital PCR Master Mix (5X) is optimized for use with the QuantStudio Absolute Q Digital PCR System and Applied Biosystems digital PCR (dPCR) assays in a simple workflow with minimal processing steps.