PCR Equipment and Supplies

Saline-Sodium Citrate (SSC), 20X Solution (Molecular Biology), Fisher BioReagents

Invitrogen™ DNA Polymerase I  

DNA polymerase with 5'→3' and 3'→5' exodeoxyribonuclease activities

Invitrogen™ Taq DNA Polymerase, native  

Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.

Invitrogen™ Uracil DNA Glycosylase  

Removes uracil residues from sugar moiety of single- and double-stranded DNA without destroying phosphodiester backbone

Invitrogen™ Taq DNA Polymerase PCR Buffer (10X)  

Taq DNA Polymerase PCR Buffer is a 10X buffer [200 mM Tris HCl (pH 8.4), 500 mM KCl] supplied with 1 mL of 50 mM MgCl₂.

Invitrogen™ SuperScript™ III Reverse Transcriptase  

Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) created by the introduction of several mutations for reduced RNase H activity, increased half-life, and improved thermal stability.

Invitrogen™ dATP (10 mM)  

Invitrogen dATP is prepared to a final concentration of 10 mM in 0.9 mM Tris-HCl at pH 7.5.

Invitrogen™ dCTP (10 mM)  

Invitrogen dCTP is prepared to a final concentration of 10 mM in 0.9 mM Tris-HCl at pH 7.5.

Invitrogen™ DNA Polymerase I/DNase I  

An optimized mixture of both enzymes for efficient nick translation of DNA

Invitrogen™ dTTP (10 mM)  

Invitrogen dTTP is prepared to a final concentration of 10 mM in 0.9 mM Tris-HCl at pH 7.5.

Invitrogen™ dGTP (10 mM)  

Invitrogen dGTP is prepared to a final concentration of 10 mM in 0.9 mM Tris-HCl at pH 7.5.

Invitrogen™ Oligo(dT)_12-18 Primer  

Oligo(dT)_12-18 Primer is suitable for use in first-strand cDNA synthesis with reverse transcriptase.

Invitrogen™ Oligo(dT)₂₀ Primer  

Used for first-strand cDNA synthesis with reverse transcriptase at temperatures of ≥50°C, Oligo(dT)₂₀ Primer is a string of 20 deoxythymidylic acid residues that hybridizes to the poly(A) tail of mRNA.

Invitrogen™ dNTP Mix (10 mM ea)  

Invitrogen 10 mM dNTP Mix includes four nucleotides (dATP, dCTP, dGTP, dTTP) in 0.6 mM Tris-HCl. Each nucleotide is at a concentration of 10 mM and this mix is suitable for use in a wide variety of molecular processes.

Invitrogen™ Biotin-14-dATP  

A patented dATP analog with biotin attached at the 6-position of the purine base by a 14-atom linker