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Real Time PCR Products

A range of products specifically designed for real-time PCR workflows, a process that monitors the amplification of a targeted DNA molecule during the PCR reaction, not at its end, as in conventional PCR. Products include assays, reagents, kits, and master mixes.
Real Time PCR Master Mixes (238)
Real Time PCR Assays and Arrays (228)
Real Time PCR Reagents and Kits (182)

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1 – 15 of 406 results
1
Promotions Available

Invitrogen™ EXPRESS qPCR Supermix, universal

EXPRESS qPCR SuperMix kits are compatible with fast-cycling instruments for probe-based qPCR assays.

GC-Rich PCR Performance High
Format Tube
Product Type Express qPCR Supermix
Sample Type DNA (Genomic), cDNA
Reaction Speed Fast or Standard
For Use With (Application) Gene Expression
PCR Method qPCR
Detection Method Primer-probe
2
Promotions Available

Invitrogen™ ROX Reference Dye

ROX Reference Dye is designed to normalize the fluorescent reporter signal in real-time quantitative PCR or RT-PCR.

Content And Storage Store in freezer (-5° to -30°C).
Label or Dye ROX
Detection Method Primer-probe
3
Promotions Available

Invitrogen™ EXPRESS qPCR Supermix, with premixed ROX

EXPRESS qPCR SuperMix kits are compatible with fast-cycling instruments for your probe-based qPCR assays.

GC-Rich PCR Performance High
Content And Storage EXPRESS qPCR SuperMix kits are provided as a 2X qPCR SuperMix with ROX premixed or in a separate tube.

Store at -20°C upon receipt. Once thawed, the EXPRESS qPCR SuperMix can be stored for up to 1 month at +4·C. Product is guaranteedstable for 6 months when properly stored.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Format Tube
Product Type Express qPCR Supermix
For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT Fast System, 7900HT System, Eppendorf™ MasterCycler™ RealPlex, StepOne™, Fast Mode, StepOne™, Standard Mode
Sample Type DNA (Genomic), cDNA
Reaction Speed Fast or Standard
For Use With (Application) Gene Expression
PCR Method qPCR
Detection Method Primer-probe
4

Invitrogen™ EXPRESS One-Step Superscript™ qRT-PCR Kit, universal

Kit incorporates proven Invitrogen™ enzymes specifically tailored to deliver superior results on high-throughput, fast-cycling instruments for probe-based qPCR experiments.

Polymerase Taq DNA Polymerase
Format Tube
Product Type qRT-PCR Kit
For Use With (Equipment) 7500 Fast System, 7500 System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, BioRad CFX96™, StepOnePlus™, Fast Mode, StepOnePlus™, Standard Mode, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, BioRad MyiQ, Corbett RotorGene, Cepheid SmartCycler, MJ Opticon, MJ Chromo4, BioRad iCycler iQ
Sample Type RNA
Reaction Speed Fast or Standard
PCR Method 1-step RT-qPCR
Product Line SuperScript
Detection Method Primer-probe
5
Promotions Available

Invitrogen™ Platinum™ Quantitative PCR SuperMix-UDG w/ROX

qPCR SuperMixes with ROX for ABI instruments are ready-to-use reaction mixes for high-specificity, real-time amplification of cDNA, genomic DNA or plasmid DNA.

Shipping Condition Dry Ice
Polymerase Platinum Taq DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2 X
Product Type Quantitative PCR SuperMix-UDG
For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT System
Sample Type DNA (Genomic), cDNA
Reaction Speed Standard
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line Platinum
Detection Method Primer-probe
6

Applied Biosystems™ resDNASEQ™ Quantitative Sf9 and Baculovirus DNA Kits

The resDNASEQ Quantitative Sf9 and Baculovirus DNA Kit is a duplex quantitative PCR (qPCR)-based system for the detection of residual DNA from the Sf9 baculovirus platform.

No. of Reactions 100 Reactions
Format Kit
Label or Dye FAM + VIC
Quantity 100 reactions
Host Cell SF9 + Bacoluvirus
PCR Method qPCR
Product Line resDNASEQ
Detection Method Primer-probe
7

Applied Biosystems™ ViralSEQ™ Lentivirus Physical Titer Kits

A one-step RT-qPCR assay for the quantitation of genome-containing lentiviral particles in supernatants from cell-based bioproduction systems. Lentiviral quantitation is a critical quality attribute (CQA) in viral vector manufacturing for cell and gene-modified cell therapy development.

Target Lentivirus genomic target
For Use With (Equipment) Real-time PCR instruments (QuantStudio5 or 7500 Fast)
Quantity 100 reactions
For Use With (Application) Virus Detection
Detection Method Primer-probe
8

Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents with manual

AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.

GC-Rich PCR Performance High
Fidelity (vs. Taq) 25 X
Description Works on all AB platforms, but is optimized for the 7500.
Reverse Transcriptase ArrayScript™
Concentration 25X
PCR Method 1-step RT-qPCR
Detection Method Primer-probe
Polymerase AmpliTaq Gold DNA Polymerase
Format Tube
Product Type One-Step RT-PCR Reagent
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 System
Reaction Speed Standard
Product Line AgPath-ID, Ambion
9

Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents

AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 25 X
Description Works on all AB platforms, but is optimized for the 7500.
Reverse Transcriptase ArrayScript™
Concentration 25X
PCR Method 1-step RT-qPCR
Detection Method Primer-probe
Polymerase AmpliTaq Gold DNA Polymerase
Format Tube
Product Type One-Step RT-PCR Reagent
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System
Sample Type RNA
Reaction Speed Standard
Product Line AgPath-ID, Ambion
10
Promotions Available

Invitrogen™ Uracil DNA Glycosylase

Removes uracil residues from sugar moiety of single- and double-stranded DNA without destroying phosphodiester backbone

11

Invitrogen™ SuperScript™ III Platinum™ One-Step qRT-PCR Kit

Combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient one-step format.

Shipping Condition Dry Ice
Polymerase Taq DNA Polymerase
Product Type One-Step qRT-PCR Kit
For Use With (Equipment) 7500 System, BioRad iCycler iQ, BioRad iQ5, Stratagene Mx4000, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Cepheid SmartCycler, BioRad MyiQ
Sample Type RNA
Reaction Speed Standard
For Use With (Application) Gene Expression,Pathogen Detection,Virus Detection
PCR Method 1-step RT-qPCR
Product Line Platinum, SuperScript
Detection Method Primer-probe
12
Promotions Available

Invitrogen™ Platinum™ Quantitative PCR SuperMix-UDG

Is a ready-to-use mixture for high-specificity, real-time quantitative DNA amplification, containing UDG to prevent carryover contamination (1,2).

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Product Type Quantitative PCR SuperMix-UDG
For Use With (Equipment) 7500 System, BioRad iCycler iQ, BioRad iQ5, Stratagene Mx4000, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Cepheid SmartCycler, BioRad MyiQ
Sample Type DNA (Genomic), cDNA
Reaction Speed Standard
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line Platinum
Detection Method Primer-probe
13

Invitrogen™ Microplate Lysis Reagent

A buffered nonionic detergent solution for the lysis of adherent or nonadherent cultured cells under non-denaturing conditions

14

Thermo Scientific™ Maxima Probe qPCR Master Mixes

Optimize qPCR with probe chemistry using standard cycling protocols with these ready-to-use qPCR master mixes.

15

Thermo Scientific™ Maxima Probe qPCR Master Mix (2X), with separate ROX vial

Optimize qPCR with probe chemistry using standard cycling protocols with these ready-to-use qPCR master mixes.

Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Format Tube
Product Type qPCR Master Mix
For Use With (Equipment) BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, MJ Chromo4, Eppendorf MasterCycler RealPlex, Corbett RotorGene, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, MJ Opticon
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line Maxima
Detection Method Primer-probe