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Real Time PCR Products

A range of products specifically designed for real-time PCR workflows, a process that monitors the amplification of a targeted DNA molecule during the PCR reaction, not at its end, as in conventional PCR. Products include assays, reagents, kits, and master mixes.
Real Time PCR Master Mixes (238)
Real Time PCR Assays and Arrays (230)
Real Time PCR Reagents and Kits (182)

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1

Applied Biosystems™ resDNASEQ™ Quantitative E. coli DNA Kits
GREENER_CHOICE

This quantitative PCR (qPCR)-based kit, part of the resDNASEQ™ E. coli Residual DNA Quantitation System, enables highly sensitive quantitation of host cell DNA from E. coli expression systems, typically in less than 4 hours.

No. of Reactions 100 Reactions
Format Kit
Label or Dye FAM
Quantity 100 reactions
Host Cell E.coli
PCR Method qPCR
Product Line resDNASEQ
Detection Method Primer-probe
2

Applied Biosystems™ QuantStudio™ 3D Digital PCR Master Mix  DFS Item

Optimized for use with isolated DNA or cDNA, TaqMan™ assays, and the QuantStudio™ 3D Digital PCR Chip

Content And Storage Remove the tube from the box, then store it at -20°C until first use, then store it at 2° to 8°C.
For Use With (Equipment) v1 chips
Concentration 2X
Detection Method Primer-probe
3

Applied Biosystems™ TaqCheck™ SARS-CoV-2 Control  DFS Item

The Applied Biosystems™ TaqCheck™ SARS-CoV-2 Control is a synthetic RNA positive control that contains target sequences for the SARS-CoV-2 S and N gene assays included in the TaqCheck™ SARS-CoV-2 Fast PCR Assay.

4

Applied Biosystems™ TaqMan™ Arbovirus Triplex Control Kit (ZIKV/DENV/CHIKV)

The Applied Biosystems™ TaqMan Zika Virus Triplex Control Kit (ZIKV/DENV/CHIKV) includes a single foil pouch that contains human DNA at 1X and pooled complete genome RNA template of multiple viruses.

5

Applied Biosystems™ Power SYBR™ Green RNA-to-CT™ 1-Step Kit  DFS Item

The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.

Shipping Condition Wet Ice
Purity or Quality Grade UP (Ultra Pure)
Reverse Transcriptase ArrayScript™ UP
For Use With (Application) Gene Expression
PCR Method 1-step RT-qPCR
Detection Method SYBR
Polymerase AmpliTaq Gold DNA Polymerase
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System
Sample Type RNA
Reaction Speed Standard
Label or Dye SYBR Green
Run Time Standard
Product Line RNA-to-CT, SYBR
6

Applied Biosystems™ TaqMan™ Sample-to-SNP™ Kit (20 mL sample prep, 10 mL PCR)  DFS Item

Go from Biological Sample to Genotyping Results in less than one hour.

Polymerase AmpliTaq DNA Polymerase
For Use With (Equipment) 7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, GeneAmp 9700, StepOne™, Standard Mode, Veriti Thermal Cycler, StepOne™ Fast Mode, StepOne™ Standard Mode
Sample Type Buccal Sample, Cell Cultures, Hair with Follicle, Leaf Punch or Needle, Seed Chip, Tail Biopsy (Mouse), Tail Biopsy (Rat), Tissue (FFPE), Whole Blood
Reaction Speed Fast or Standard
Detection Method Primer-probe
7

VetMAX™-Gold AIV Detection Kit  DFS Item

The VetMAX™-Gold AIV Detection Kit (Avian Influenza Virus RNA Test Kit) is a highly sensitive, qualitative, one-step, real-time RT-PCR assay to detect Avian Influenza Virus (AIV) RNA isolated from poultry (chicken/turkey) oropharyngeal/tracheal swab samples.

9

Applied Biosystems™ resDNASEQ™ Quantitative Plasmid DNA - Kanamycin Resistance Gene Kits
GREENER_CHOICE

The resDNASEQ Quantitative Plasmid DNA - Kanamycin Resistance Gene Kit is a quantitative PCR (qPCR)-based system for the detection of residual kanamycin-resistant plasmids.

No. of Reactions 100 Reactions
Target Kanamycin Resistant Plasmids
Format Kit
Label or Dye FAM
Quantity 100 reactions
PCR Method qPCR
Product Line resDNASEQ
Detection Method Primer-probe
10

Applied Biosystems™ resDNASEQ™ dPCR E1A DNA Fragment Length Kit
GREENER_CHOICE

The resDNASEQ dPCR E1A DNA Fragment Length Kit is an absolute quantitation PCR-based system that measures the fragment lengths of residual host-cell DNA by targeting the E1A gene in HEK 293 cell lines, which are predominantly used in the development of gene therapies, cell-based vaccines, and similar biotherapeutics.

11

Applied Biosystems™ resDNASEQ™ Quantitative MDCK DNA Kits
GREENER_CHOICE

The resDNASEQ™ Quantitative MDCK DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from MDCK (canine kidney) cells, an expression system commonly used for the production of vaccines.

No. of Reactions 100 Reactions
Format Kit
Label or Dye FAM
Quantity 100 reactions
Host Cell MDCK
PCR Method qPCR
Product Line resDNASEQ
Detection Method Primer-probe
13

Applied Biosystems™ resDNASEQ™ Quantitative CHO DNA Kits
GREENER_CHOICE

This quantitative PCR (qPCR)-based kit, part of the resDNASEQ™ Quantitative CHO DNA System, allows users to detect sub-picogram amounts of residual DNA from CHO cell line in under 5 hours.

No. of Reactions 100 Reactions
Format Kit
Label or Dye FAM
Quantity 100 reactions
Host Cell CHO
PCR Method qPCR
Product Line resDNASEQ
Detection Method Primer-probe
14

Applied Biosystems™ TaqMan™ Multiplex Master Mix  DFS Item

Applied Biosystems™ TaqMan™ Multiplex Master Mix is formulated to amplify up to 4 targets in a single reaction, delivering shorter run times (less than 40 minutes).

Polymerase AmpliTaq Fast DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2 X
Product Type Multiplex Master Mix
Sample Type DNA
Reaction Speed Fast
Concentration 2X
For Use With (Application) Gene Expression,Virus Detection
PCR Method Multiplex qPCR
Product Line TaqMan
Detection Method Primer-probe
15

Applied Biosystems™ TaqPath™ 1-Step RT-qPCR Master Mix, CG  DFS Item

TaqPath 1-Step RT-qPCR Master Mix, CG, is designed for robust and reproducible one-step pathogen detection and gene expression workflows. The single-tube 4X formulation contains thermostable MMLV reverse transcriptase, dNTPs, UNG, ROX dye, and thermostable Fast DNA polymerase.

Reverse Transcriptase M-MLV
Form Liquid
Concentration 4X
For Use With (Application) Microbial Detection,DNA Quantification,Presence/Absence
PCR Method 1-step RT-qPCR
Multiplex Capability Up to 3-plex
Detection Method Primer-probe
Includes dUTP, UNG/UDG
Thermostability Not Applicable
Polymerase Fast DNA Polymerase
Sensitivity Down to 10 copies
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System, 7500 Fast Dx System, QuantStudio™ Dx
Sample Type RNA, cDNA, DNA
Reaction Speed Fast or Standard
Inhibitor Tolerance (Simple) High
Target Specificity RNA only, Both RNA and DNA
Hot Start Built-In Hot Start
Product Line TaqPath