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Real Time PCR Products

A range of products specifically designed for real-time PCR workflows, a process that monitors the amplification of a targeted DNA molecule during the PCR reaction, not at its end, as in conventional PCR. Products include assays, reagents, kits, and master mixes.
Real Time PCR Master Mixes (238)
Real Time PCR Assays and Arrays (228)
Real Time PCR Reagents and Kits (183)

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1 – 15 of 407 results
1

Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents

AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 25 X
Description Works on all AB platforms, but is optimized for the 7500.
Reverse Transcriptase ArrayScript™
Concentration 25X
PCR Method 1-step RT-qPCR
Detection Method Primer-probe
Polymerase AmpliTaq Gold DNA Polymerase
Format Tube
Product Type One-Step RT-PCR Reagent
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System
Sample Type RNA
Reaction Speed Standard
Product Line AgPath-ID, Ambion
2

Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents with manual

AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.

GC-Rich PCR Performance High
Fidelity (vs. Taq) 25 X
Description Works on all AB platforms, but is optimized for the 7500.
Reverse Transcriptase ArrayScript™
Concentration 25X
PCR Method 1-step RT-qPCR
Detection Method Primer-probe
Polymerase AmpliTaq Gold DNA Polymerase
Format Tube
Product Type One-Step RT-PCR Reagent
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 System
Reaction Speed Standard
Product Line AgPath-ID, Ambion
4
Promotions Available

Applied Biosystems™ TaqPath™ qPCR Master Mix, CG

TaqPath qPCR Master Mix, CG, is a 2X formulation designed for gene expression and miRNA analysis. It contains thermostable Fast DNA polymerase, uracil-N-glycosylase (UNG), dNTPs with dUTP, ROX dye (passive reference), and optimized buffer components for maximum robustness and reproducibility.

Content And Storage Store at 2°C to 8°C in the dark.
Form Liquid
InhibitorToleranceSimple High
Concentration 2X
For Use With (Application) Gene Expression,Microbial Detection,DNA Quantitation,Presence/Absence,miRNA analysis
PCR Method qPCR
Multiplex Capability Duplex
Detection Method Primer-probe
Includes dUTP, UNG/UDG
Thermostability 48 hr., Assembled Reaction
Polymerase Fast DNA Polymerase
Sensitivity Down to 10 copies
Format Bottle
Product Type qPCR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) QuantStudio Dx, 7500 Fast Dx
Sample Type cDNA, DNA
Reaction Speed Fast or Standard
Target Specificity cDNA or gDNA only
Hot Start Built-In Hot Start
Product Line TaqPath
5

Invitrogen™ SuperScript™ III Platinum™ One-Step qRT-PCR Kit

Combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient one-step format.

Shipping Condition Dry Ice
Polymerase Taq DNA Polymerase
Product Type One-Step qRT-PCR Kit
For Use With (Equipment) 7500 System, BioRad iCycler iQ, BioRad iQ5, Stratagene Mx4000, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Cepheid SmartCycler, BioRad MyiQ
Sample Type RNA
Reaction Speed Standard
For Use With (Application) Gene Expression,Pathogen Detection,Virus Detection
PCR Method 1-step RT-qPCR
Product Line Platinum, SuperScript
Detection Method Primer-probe
6
Promotions Available

Invitrogen™ EXPRESS qPCR Supermix, universal

EXPRESS qPCR SuperMix kits are compatible with fast-cycling instruments for probe-based qPCR assays.

GC-Rich PCR Performance High
Format Tube
Product Type Express qPCR Supermix
Sample Type DNA (Genomic), cDNA
Reaction Speed Fast or Standard
For Use With (Application) Gene Expression
PCR Method qPCR
Detection Method Primer-probe
7
Promotions Available

Invitrogen™ ROX Reference Dye

ROX Reference Dye is designed to normalize the fluorescent reporter signal in real-time quantitative PCR or RT-PCR.

Content And Storage Store in freezer (-5° to -30°C).
Label or Dye ROX
Detection Method Primer-probe
8
Promotions Available

Applied Biosystems™ TaqMan™ Exogenous Internal Positive Control Reagents
GREENER_CHOICE

TaqMan™ Exogenous Internal Positive Control Reagents contain a pre-optimized internal positive control (IPC) with pre-designed primers and TaqMan™ probe.

GC-Rich PCR Performance Low
Format Tube(s)
Internal Probe Modification TAMRA™ Quencher (3'), VIC™ (5')
Product Type Exogenous Internal Positive Control Reagent
Reaction Speed Standard
Green Features Sustainable packaging
PCR Method qPCR
Product Line TaqMan
9
Promotions Available

Applied Biosystems™ Human B2M (Beta-2-Microglobulin) Endogenous Control (FAM™/MGB probe, non-primer limited)
GREENER_CHOICE

Human B2M (Beta-2-Microglobulin) Endogenous Control (FAM™/MGB probe, non-primer limited)

Shipping Condition Room Temperature
Content And Storage Control Primer-Probe Set(s)
Gene Symbol B2M
Green Features Sustainable packaging
Amplicon Size 75
Concentration 20X
For Use With (Application) Gene Expression
PCR Method 2-step RT-qPCR
Detection Method Primer-probe
Accession Number NM_004048.2
Target B2M
Format Tube
Label or Dye FAM
Species Human
Product Line FAM, TaqMan
10
Promotions Available

Applied Biosystems™ Custom TaqMan™ Gene Expression Assay, FAM
GREENER_CHOICE

Applied Biosystems Custom TaqMan Gene Expression assays are the custom version of our predesigned TaqMan assays for quantitative real-time PCR.

Shipping Condition Room Temperature
Content And Storage 1 tube containing a 20X (S and M sizes) or 60X (L size) mix of pre-formulated assay (1 probe and 2 primers).

Store at -15 to -25°C.
Product Type Custom TaqMan Gene Expression
For Use With (Equipment) 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex
Green Features Sustainable packaging
Product Line TaqMan
11
Promotions Available

Applied Biosystems™ Eukaryotic 18S rRNA Endogenous Control (FAM™/MGB probe, non-primer limited)
GREENER_CHOICE

Eukaryotic 18S rRNA Endogenous Control (FAM™/MGB probe, non-primer limited)

Shipping Condition Room Temperature
Content And Storage Control Primer-Probe Set(s)
Gene Symbol 18S
Green Features Sustainable packaging
Amplicon Size 187
Concentration 20X
For Use With (Application) Gene Expression
PCR Method 2-step RT-qPCR
Detection Method Primer-probe
Accession Number X03205.1
Target 18S rRNA
Format Tube
Label or Dye FAM
Species Eukaryotic, Rat, Mouse, Human
Product Line FAM, TaqMan
12

Applied Biosystems™ Human HPRT1 (HGPRT) Endogenous Control (FAM™/MGB probe, non-primer limited)

Human HPRT1 (HGPRT) Endogenous Control (FAM™/MGB probe, non-primer limited)

For Use With (Application) Gene Expression
PCR Method 2-step RT-qPCR
Detection Method Primer-probe
13
Promotions Available

Applied Biosystems™ Human PPIA (Cyclophilin A) Endogenous Control (FAM™/MGB probe, non-primer limited)

Human PPIA (Cyclophilin A) Endogenous Control (FAM™/MGB probe, non-primer limited)

For Use With (Application) Gene Expression
PCR Method 2-step RT-qPCR
Detection Method Primer-probe
14
Promotions Available

Applied Biosystems™ TaqMan™ Array, Format 48

The Custom TaqMan™ Array Card is a 384-well microfluidic card designed to perform 384 simultaneous real-time PCR reactions without using liquid-handling robots or multichannel pipettors.

15
Promotions Available

Applied Biosystems™ Mouse ACTB (Actin, Beta) Endogenous Control (VIC™/MGB probe, primer limited)
GREENER_CHOICE

Mouse ACTB (Actin, Beta) Endogenous Control (VIC™/MGB probe, primer limited)