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Real Time PCR Products

A range of products specifically designed for real-time PCR workflows, a process that monitors the amplification of a targeted DNA molecule during the PCR reaction, not at its end, as in conventional PCR. Products include assays, reagents, kits, and master mixes.
Real Time PCR Master Mixes (238)
Real Time PCR Assays and Arrays (229)
Real Time PCR Reagents and Kits (183)

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1

Applied Biosystems™ resDNASEQ™ dPCR CHO DNA Kit
GREENER_CHOICE

The Applied Biosystems resDNASEQ dPCR CHO DNA Kit is a powerful digital PCR (dPCR) solution for quantitating residual DNA from CHO expression systems, which are commonly utilized for the production of monoclonal antibodies (mAbs).

2

Applied Biosystems™ ViralSEQ™ Lentivirus Physical Titer Kit, 100 reactions  DFS Item

A one-step RT-qPCR assay for the quantitation of genome-containing lentiviral particles in supernatants from cell-based bioproduction systems. Lentiviral quantitation is a critical quality attribute (CQA) in viral vector manufacturing for cell and gene-modified cell therapy development.

3

Applied Biosystems™ ViralSEQ™ Quantitative Sf-Rhabdovirus Kits
GREENER_CHOICE

The Applied Biosystems ViralSEQ Quantitative Sf-Rhabdovirus Kit is a two-step RT-qPCR assay for the detection of Sf-rhabdovirus in recombinant products manufactured in insect cell lines using baculovirus expression systems.

Shipping Condition Dry Ice
Target Sf-rhabdovirus
Quantity 100 reactions
PCR Method qPCR
Product Line ViralSEQ
Detection Method Primer-probe
4
Promotions Available

Applied Biosystems™ TaqMan™ Advanced miRNA cDNA Synthesis Kit  DFS Item

Kit uses 3' poly-A tailing and 5' ligation of an adaptor sequence to extend the mature miRNAs present in the sample on each end prior to reverse transcription.

5

Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents with manual  DFS Item

AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.

GC-Rich PCR Performance High
Fidelity (vs. Taq) 25 X
Description Works on all AB platforms, but is optimized for the 7500.
Reverse Transcriptase ArrayScript™
Concentration 25X
PCR Method 1-step RT-qPCR
Detection Method Primer-probe
Polymerase AmpliTaq Gold DNA Polymerase
Format Tube
Product Type One-Step RT-PCR Reagent
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 System
Reaction Speed Standard
Product Line AgPath-ID, Ambion
6

Applied Biosystems™ resDNASEQ™ Quantitative Sf9 and Baculovirus DNA Kits
GREENER_CHOICE

The resDNASEQ Quantitative Sf9 and Baculovirus DNA Kit is a duplex quantitative PCR (qPCR)-based system for the detection of residual DNA from the Sf9 baculovirus platform.

No. of Reactions 100 Reactions
Format Kit
Label or Dye FAM + VIC
Quantity 100 reactions
Host Cell SF9 + Bacoluvirus
PCR Method qPCR
Product Line resDNASEQ
Detection Method Primer-probe
7

Applied Biosystems™ ViralSEQ™ Lentivirus Physical Titer Kits
GREENER_CHOICE

A one-step RT-qPCR assay for the quantitation of genome-containing lentiviral particles in supernatants from cell-based bioproduction systems. Lentiviral quantitation is a critical quality attribute (CQA) in viral vector manufacturing for cell and gene-modified cell therapy development.

Target Lentivirus genomic target
For Use With (Equipment) Real-time PCR instruments (QuantStudio5 or 7500 Fast)
Quantity 100 reactions
For Use With (Application) Virus Detection
Detection Method Primer-probe
8

6-Tamra NHS/DMSO  DFS Item

The single-isomer preparations of TAMRA are most important for high-resolution techniques such as DNA sequencing and capillary electrophoresis

9
Promotions Available

Applied Biosystems™ TaqMan™ PreAmp Master Mix Kit  DFS Item

TaqMan™ PreAmp Master Mix Kit contains TaqMan™ PreAmp Master Mix and TaqMan™ Gene Expression Master Mix.

Content And Storage Contains 2X TaqMan PreAmp Master Mix (1 mL), TaqMan™ Gene Expression Master Mix (5 mL), and TaqMan™ PreAmp Master Mix Kit Quick Reference Card. Store master mixes at 2-8°C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
For Use With (Equipment) 7300 System, 7500 Fast System, 7500 System, 7900HT Fast System
Sample Type cDNA
Detection Method Primer-probe
10

Applied Biosystems™ MeltDoctor™ HRM Reagent Kit  DFS Item

Provides individually packaged PCR components and the MeltDoctor™ HRM Dye required for high resolution melting analysis.

13

Applied Biosystems™ resDNASEQ™ Quantitative HEK293 DNA Kits
GREENER_CHOICE

The resDNASEQ Quantitative HEK293 DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host-cell DNA from HEK293 cell lines.

No. of Reactions 100 Reactions
Format Kit
Label or Dye FAM
Quantity 100 reactions
Host Cell HEK
PCR Method qPCR
Product Line resDNASEQ
Detection Method Primer-probe
14

Applied Biosystems™ resDNASEQ™ Human Residual DNA Quantitation Kits
GREENER_CHOICE

The resDNASEQ Human Residual DNA Quantitation Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from human cell lines used as expression systems for recombinant proteins.

No. of Reactions 100 Reactions
Format Kit
Label or Dye FAM
Quantity 100 reactions
Host Cell Human DNA
PCR Method qPCR
Product Line resDNASEQ
Detection Method Primer-probe
15
Promotions Available

Applied Biosystems™ TaqMan™ Reagents Starter Kit  DFS Item

TaqMan™ PCR Starter Kit is designed to detect and quantify Eukaryotic 18S rRNA sequence in human Raji cDNA using the TaqMan™ 5' nuclease assay with the TaqMan™ PCR Master Mix.

Sample Type DNA (Genomic), cDNA
Reaction Speed Standard
For Use With (Application) Gene Expression
Detection Method Primer-probe