Applied Biosystems™ MeltDoctor™ HRM Master Mix 

Description

High Resolution Melting (HRM) analysis is a post-PCR analysis method used to identify genetic variation in nucleic acid sequences. Unlike some competitive mixes, the MeltDoctor™ HRM Master Mix does not require additional mixing prior to use, and was developed and optimized solely for HRM applications.

• Low background fluorescence
• High brightness in the presence of double-stranded DNA
• Minimal temperature shift of DNA melting due to dye binding
• Thermal stability to tolerate PCR cycling conditions
• No inhibition of polymerase activity, resulting in high PCR efficiency
• A dNTP blend including dUTP, which minimizes carryover contamination by allowing amplicon degradation by uracil DNA glycosylase (UDG) in subsequent PCR reactions

• HRM analysis can be used to scan for mutations in target genes for the identification of variant samples prior to sequencing analysis
• As a mutation scanning technique, HRM offers significant advantages over conventional methods such as Denaturing High- Performance Liquid Chromatography (DHPLC) and Denaturing Gradient Gel Electrophoresis (DGGE)

• Low reagent consumption, with little waste: HRM requires only a 20μL PCR reaction for analysis of each sample, eliminating the need for HPLC solvents or DGGE gels
• Simple, fast workflow: no additional instrumentation is required after PCR amplification;A high-resolution melt step can be simply added to the end of the PCR profile for immediate analysis
• Fast optimization: unlike DHPLC, thermal optimization is not required
• Low sample consumption: following HRM analysis, the PCR product can be used directly in a Sanger sequencing reaction
  Quality Components
  
  • Magnesium salts and other buffer components, precisely formulated to obtain optimal HRM results
  • AmpliTaq Gold™ 360 DNA Polymerase, a highly purified DNA polymerase that provides hot-start performance, minimizing non-specific product formation and enabling reactions to be set up at room temperature
  • MeltDoctor™ HRM Dye, a stabilized form of the fluorescent SYTO™ 9 double-stranded nucleic acid stain developed by Molecular Probes

  Order Info

  Guaranteed minimum shelf life is 60 days. Shipping Conditions: Wet Ice