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Applied Biosystems™ MeltDoctor™ HRM Master Mix DFS Item

Formulated for superior HRM performance across a wide range of genomic targets

$642.60 - $5,181.75

Specifications

For Use With (Application) 7500 Fast System, 7900HT Fast System, QuantStudio™ 12K Flex, StepOne™, StepOnePlus™, ViiA™ 7 System
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Includes:  Provided in a convenient 2X mix, containing: AmpliTaq Gold™ 360 DNA Polymerase, MeltDoctor HRM Dye, a dNTP blend including dUTP, Magnesium salts and other buffer components, precisely formulated to obtain optimal HRM results
Requires:  Template and primers

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

4409535

DFS Item
 
life technologies
4409535
50mL Each for $5,097.75

4415440

DFS Item
 
life technologies
4415440
5mL Each for $642.60

4415450

DFS Item
 
life technologies
4415450
10 x 5mL Each for $5,181.75

4415452

DFS Item
 
life technologies
4415452
5 x 5mL Each for $2,709.00
Description & Specifications

Specifications

For Use With (Application) 7500 Fast System, 7900HT Fast System, QuantStudio™ 12K Flex, StepOne™, StepOnePlus™, ViiA™ 7 System

MeltDoctor HRM Master Mix contains all components (except template and primers) for High Resolution Melting analysis, a post-PCR analysis method used to identify genetic variation in nucleic acid sequences. No additional mixing required prior to use.

  • Low background fluorescence
  • High brightness in the presence of double-stranded DNA
  • Minimal temperature shift of DNA melting due to dye binding
  • Thermal stability to tolerate PCR cycling conditions
  • No inhibition of polymerase activity, resulting in high PCR efficiency
  • A dNTP blend including dUTP, which minimizes carryover contamination by allowing amplicon degradation by uracil DNA glycosylase (UDG) in subsequent PCR reactions

  • Perform Mutation Scanning HRM analysis can be used to scan for mutations in target genes for the identification of variant samples prior to sequencing analysis. As a mutation scanning technique, HRM offers significant advantages over conventional methods such as Denaturing High-Performance Liquid Chromatography (DHPLC) and Denaturing Gradient Gel Electrophoresis (DGGE). Specifically, the advantages of HRM for mutation scanning include: •Low reagent consumption, with little waste: HRM requires only a 20μL PCR reaction for analysis of each sample, eliminating the need for HPLC solvents or DGGE gels
  • Simple, fast workflow: no additional instrumentation is required after PCR amplification. A high-resolution melt step can be simply added to the end of the PCR profile for immediate analysis
  • Fast optimization: unlike DHPLC, thermal optimization is not required
  • Low sample consumption: following HRM analysis, the PCR product can be used directly in a Sanger sequencing reaction Quality Components
  • Magnesium salts and other buffer components, precisely formulated to obtain optimal HRM results
  • AmpliTaq Gold™ 360 DNA Polymerase, a highly purified DNA polymerase that provides hot-start performance, minimizing nonspecific product formation and enabling reactions to be set up at room temperature
  • MeltDoctor HRM Dye, a stabilized form of the fluorescent SYTO™ 9 double-stranded nucleic acid stain developed by Molecular Probes

Gene Expression Analysis & Genotyping, Genetic Variation Analysis Using Real-Time PCR, Genotyping & Genomic Profiling, High Resolution Melting (HRM) Analysis, Methylation Analysis, Methylation-Sensitive HRM Analysis, PCR & Real-Time PCR, RNAi, Epigenetics & Noncoding RNA Research, Real Time PCR (qPCR)

Shipping Condition: Wet Ice

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