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Applied Biosystems™ MeltDoctor™ HRM Master Mix DFS Item

Contains all components (excluding template and primers) formulated for superior HRM performance across a wide range of genomic targets.

$662.00 - $5,340.00

Specifications

For Use With (Application) 7500 Fast System, 7900HT Fast System
View More Specs

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Includes: Provided in a convenient 2X mix, containing: AmpliTaq Gold™ 360 DNA Polymerase, MeltDoctor HRM Dye, a dNTP blend including dUTP, Magnesium salts and other buffer components, precisely formulated to obtain optimal HRM results

Requires: Template and primers



Products ${productFamilyLength}
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
4409535 Applied Biosystems™
LS4409535
50mL Each for $5,250.00
4415440 Applied Biosystems™
LS4415440
5mL Each for $662.00
4415450 Applied Biosystems™
LS4415450
10 x 5mL Each for $5,340.00
4415452 Applied Biosystems™
LS4415452
5 x 5mL Each for $2,790.00
Specifications

Description

  • Low background fluorescence
  • High brightness in the presence of double-stranded DNA
  • Minimal temperature shift of DNA melting due to dye binding
  • Thermal stability to tolerate PCR cycling conditions
  • No inhibition of polymerase activity, resulting in high PCR efficiency
  • A dNTP blend including dUTP, which minimizes carryover contamination by allowing amplicon degradation by uracil DNA glycosylase (UDG) in subsequent PCR reactions

Perform Mutation Scanning:
  • HRM analysis can be used to scan for mutations in target genes for the identification of variant samples prior to sequencing analysis
  • As a mutation scanning technique, HRM offers significant advantages over conventional methods such as Denaturing High- Performance Liquid Chromatography (DHPLC) and Denaturing Gradient Gel Electrophoresis (DGGE)

Advantages of HRM for mutation scanning
  • Low reagent consumption, with little waste: HRM requires only a 20μL PCR reaction for analysis of each sample, eliminating the need for HPLC solvents or DGGE gels
  • Simple, fast workflow: no additional instrumentation is required after PCR amplification;A high-resolution melt step can be simply added to the end of the PCR profile for immediate analysis
  • Fast optimization: unlike DHPLC, thermal optimization is not required
  • Low sample consumption: following HRM analysis, the PCR product can be used directly in a Sanger sequencing reaction
    Quality Components
    • Magnesium salts and other buffer components, precisely formulated to obtain optimal HRM results
    • AmpliTaq Gold™ 360 DNA Polymerase, a highly purified DNA polymerase that provides hot-start performance, minimizing non-specific product formation and enabling reactions to be set up at room temperature
    • MeltDoctor™ HRM Dye, a stabilized form of the fluorescent SYTO™ 9 double-stranded nucleic acid stain developed by Molecular Probes

    Order Info

    Guaranteed minimum shelf life is 60 days. Shipping Conditions: Wet Ice

Description & Specifications

Specifications

7500 Fast System, 7900HT Fast System