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  7. Applied Biosystems™ TaqMan™ Environmental Master Mix 2.0

Applied Biosystems™ TaqMan™ Environmental Master Mix 2.0

Catalog No. p-6833194
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Catalog No. No. of Reactions
4396838 200 Reactions
4398021 400 Reactions
4398044 800 Reactions
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Catalog No. 4396838 Supplier Applied Biosystems™ Supplier No. 4396838
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Description

Accurate, real-time PCR-based pathogen detection in the presence of high levels of inhibitors.

Applied Biosystems™ TaqMan™ Environmental Master Mix 2.0 offers accurate, real-time PCR-based pathogen detection in the presence of high levels of inhibitors. Use this Master Mix to analyze environmental, food and other challenging samples.

  • Tailored for quantitative, real-time PCR experiments
  • Unrivalled sensitivity for challenging applications, low copy number target detection and multiplex PCR
  • Includes pathogen detection and viral⁄bacterial load quantification
  • Sensitive detection for reliable quantification of abundant and limited genetic targets
  • Stable mix for high-throughput handling
  • Upon receipt, store at -15°C to -25°C before first use, protected from light
  • After first use, store at 2°C to 8°C, protected from light

Optimized Formulation for Unrivalled Performance

TaqMan Environmental Master Mix 2.0 is a convenient 2X mix for target quantification that includes:

  • AmpliTaq Gold™ DNA Polymerase, UP (Ultra Pure), a highly purified DNA Polymerase for improved detection of bacterial targets. This hot-start enzyme is inactive at room temperature so reactions can be set up on the benchtop. Enzyme is activated during thermal cycling.
  • Passive internal reference based on proprietary ROX™ dye for precise data analysis.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration 2X
Content And Storage This TaqMan Environmental Master Mix 2.0 is a convenient 2X mix in a 5 mL bottle that includes: AmpliTaq Gold DNA Polymerase, UP (Ultra Pure), and a passive internal reference based on proprietary ROX dye for precise data analysis.
Upon receipt, store at -15 to -25°C before first use, protected from light. After first use, store at 2 to 8°C, protected from light.
Detection Method Primer-probe
Format Bottle
PCR Method qPCR
Polymerase AmpliTaq Gold DNA Polymerase
Reaction Speed Standard
For Use With (Equipment) 7000 System, 7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, StepOne™, Standard Mode
Passive Reference Dye ROX (Pre-mixed)
Product Line TaqMan
Product Type qPCR Master Mix
Purity or Quality Grade UP (Ultra Pure)
Quantity 200 reactions
Shipping Condition Dry Ice
Sufficient For 200 Reactions
For Use With (Application) Gene Expression, Genotyping, Pathogen Detection, Virus Detection
Hot Start Built-In Hot Start
No. of Reactions 200 Reactions
Sample Type Environmental Sample, Food
Volume 5 mL
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Frequently Asked Questions (FAQs)

Is the 2X Environmental Master Mix the same as the TaqMan Universal PCR Master Mix, with or without UNG?

No, the 2X Environmental Master Mix (EMM) is not the same as the TaqMan Universal PCR Master Mix (with or without AmpErase UNG). The 2X Environmental Master Mix is specifically formulated to detect specific bacterial pathogens with greater specificity and sensitivity. It includes AmpliTaq Gold DNA Polymerase, LD, which is a highly purified version of AmpliTaq Gold DNA Polymerase. Every effort has been made in the development of this new polymerase to eliminate bacterial DNA from the enzyme preparation.
Is the TaqMan Environmental Master Mix 2.0 (Cat. No. 4398021, 4398044, 4396838) compatible with the QuantStudio instruments?

Yes, it is.
I have run out of Environmental Master Mix v2.0 from the Ion 16S Metagenomics Kit (Cat. No. A26216). Do you offer this as a standalone item?

Yes. You can purchase the TaqMan Environmental Master Mix 2.0 (Cat. No. 4396838).
How many PCR reactions can I perform using the 2X Environmental Master Mix within the Ion 16S Metagenomics Kit (Cat. No. A26216)?

It is sufficient for 100 PCR reactions.
Is there a mastermix that I can use with fecal samples?

For fecal samples, we recommend a master mix that is very tolerant of inhibitors, such as our TaqMan Environmental Master Mix 2.0. Please refer to the following paper: https://assets.thermofisher.com/TFS-Assets/CMD/posters/cms_089094.pdf
Is the TaqMan Environmental Master Mix 2.0 that is used in the Ion 16S Metagenomics Kit available separately?

Yes, the TaqMan Environmental Master Mix 2.0 is available separately as Cat. No. 4396838.

For Research Use Only. Not for use in diagnostic procedures.