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PCR Supplies

A variety of products designed for use in various PCR procedures. Includes dNTPs, enzymes, assays, arrays, master mixes, reagents, and kits. Products can be used for standard, real-time, direct, high fidelity, hot start, and long range PCR protocols.
Real Time PCR Products (649)
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DNA Polymerases (69)
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1 – 15 of 724 results
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Saline-Sodium Citrate (SSC), 20X Solution (Molecular Biology), Fisher BioReagents

Physical Form Liquid
pH 7
Chemical Name or Material Saline-Sodium Citrate
Grade Molecular Biology
DNase DNase free
Recommended Storage RT
Concentration 2.9 to 3.1 M
2

Invitrogen™ Biotin-14-dATP

A patented dATP analog with biotin attached at the 6-position of the purine base by a 14-atom linker

4

MilliporeSigma™ Novagen™ NovaTaq™ Hot Start Master Mix Kits

Premixed 2X hot start PCR components for convenience and reproducibility

Content And Storage Ready-to-use 2X mixture of NovaTaq Hot Start DNA Polymerase, ultrapure deoxynucleotides, and reaction buffer with MgCl2, PCR Grade Water, MgCl2 for optimizing Mg2+ concentrations
Product Type Hot start master mix kit
7

Applied Biosystems™ TaqMan™ Vaginal Microbiota Amplification Control

The TaqMan™ Vaginal Microbiota Amplification Control is designed to evaluate the amplification efficacy of the TaqMan Vaginal Microbiota assays. This plasmid serves as a positive control for the TaqMan Vaginal Microbiota Assay collection.

Shipping Condition Gel Packs
Content And Storage Store at -5 to -30°C.
Label or Dye Unlabeled
Concentration 105 Copies/μL
Product Line TaqMan
8

Thermo Scientific™ RapidFinder™ Sheep ID Kit

The RapidFinder™ Sheep ID Kit enables the simple and reliable detection of sheep and lamb in food and feed samples. The method works by detection of ovine, or Ovis aries, DNA by real-time PCR.

9

Thermo Scientific™ dNTP Mix (20 mM)

Thermo Scientific dNTP Mix (20 mM) contains purified deoxyribonucleotide triphosphates (dNTPs) that meet high quality standards that result in excellent performance for a variety of molecular biology applications.

10

Thermo Scientific™ Verso cDNA Synthesis Kit

The Thermo Scientific Verso cDNA Synthesis Kit provides robust transcription of RNA to create a complete cDNA pool.

Shipping Condition Wet or Dry Ice
Format Kit
Reverse Transcriptase Verso
Size (Final Product) Up to 11 kb
Reaction Format Separate components
Final Product Type First-Strand cDNA
Reaction Speed 30 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Yes
Optimal Reaction Temperature 42°C
Technique Reverse Transcription
Starting Material RNA
For Use With (Application) Real Time PCR (qPCR),RT-PCR
11

Applied Biosystems™ resDNASEQ™ Quantitative Sf9 and Baculovirus DNA Kits

The resDNASEQ Quantitative Sf9 and Baculovirus DNA Kit is a duplex quantitative PCR (qPCR)-based system for the detection of residual DNA from the Sf9 baculovirus platform.

No. of Reactions 100 Reactions
Format Kit
Label or Dye FAM + VIC
Quantity 100 reactions
Host Cell SF9 + Bacoluvirus
PCR Method qPCR
Product Line resDNASEQ
Detection Method Primer-probe
12

Applied Biosystems™ ViralSEQ™ Lentivirus Physical Titer Kits

A one-step RT-qPCR assay for the quantitation of genome-containing lentiviral particles in supernatants from cell-based bioproduction systems. Lentiviral quantitation is a critical quality attribute (CQA) in viral vector manufacturing for cell and gene-modified cell therapy development.

Target Lentivirus genomic target
For Use With (Equipment) Real-time PCR instruments (QuantStudio5 or 7500 Fast)
Quantity 100 reactions
For Use With (Application) Virus Detection
Detection Method Primer-probe
13

Thermo Scientific™ Phire Hot Start II DNA Polymerase

Thermo Scientific Phire Hot Start II DNA Polymerase is an enhanced PCR enzyme for routine and high throughput PCR applications. It is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields.

Shipping Condition Wet or Dry Ice
Polymerase Phire Hot Start II DNA Polymerase
GC-Rich PCR Performance Medium
Fidelity (vs. Taq) 2X
Reaction Format Separate Components
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
Starting Material DNA
For Use With (Application) Cloning,Hot-start PCR
14

Thermo Scientific™ Phire Hot Start II PCR Master Mixes

Thermo Scientific Phire Hot Start II PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps. The master mix contains Phire Hot Start II DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2.

Shipping Condition Wet or Dry Ice
Polymerase Phire Hot Start II DNA Polymerase
GC-Rich PCR Performance Medium
Fidelity (vs. Taq) 2X
Reaction Format SuperMix or Master Mix
Product Type Hot Start PCR Master Mix
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
Concentration 2X
Starting Material DNA
For Use With (Application) Cloning,Hot-start PCR
15

Thermo Scientific™ Phire Plant Direct PCR Kit (without sampling tools)

Thermo Scientific Phire Plant Direct PCR Kit is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR.