missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. PCR Equipment and Supplies
  4. PCR Supplies
  5. Real Time PCR Products
  6. Real Time PCR Assays and Arrays
  7. A50137

Applied Biosystems™ TaqMan™ Microbe Detection Assay

Catalog No. A50137
Encompass
Change view
Click to view available options
Quantity:
250 μL
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
A50137 250 μL
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. A50137 Supplier Applied Biosystems™ Supplier No. A50137
Only null left

Description

Applied Biosystems TaqMan Microbe Detection and TaqMan Antibiotic Resistance assays are used for quantitative real-time PCR detection of microbial species and antibiotic resistance genes.

  • Assays are used for quantitative real-time PCR detection of microbial species and antibiotic resistance genes
  • These assays consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (FAM) on the 5’ end and a minor groove binder (MGB) and non-fluorescent quencher (NFQ) on the 3’ end
  • All of our predesigned TaqMan gene expression assays are covered by the TaqMan Assays qPCR Guarantee

Features of TaqMan Microbe Detection assays include:

  • Easy to use - just add DNA and master mix and run the qPCR, no melt curves required
  • Specific - TaqMan assays use proprietary MGB-containing probes that can be up to 15 bases shorter than non-MGB probes, improving the specificity of the assay
  • Sensitive - TaqMan assays are ideal for measuring low-abundance microbial targets
  • Accurate - identify microbial targets with high accuracy of quantitation
  • Extensive content - over 300 pre-designed microbe detection assays and 100 pre-designed antibiotic resistance assays currently available
  • Gold-standard TaqMan qPCR chemistry - TaqMan assays draw on Thermo Fisher Scientific's bioinformatics assay design pipeline to help ensure high specificity and minimal cross-reactivity
  • Fast delivery - assays shipped in as little as 1–2 days (see below)

Specifications

3'Primer Modification FAM
Concentration 20X
5'Primer Modification MGB-NFQ
Content And Storage Store at -15°C to -25°C.
Detection Method Primer-probe
Format Tube
Product Line TaqMan
Quantity 250 μL
Shipping Condition Room temperature
For Use With (Application) Pathogen Detection
No. of Reactions 250 Reactions
Show More Show Less

Frequently Asked Questions (FAQs)

What are the concentrations of the primers and probes for TaqMan microbial assays?

Our predesigned and custom TaqMan microbial 20X assays have a primer concentration of 900 nM and a probe concentration of 250 nM in the final 1X concentration.

For TaqMan Microbe Detection and Antibiotic Resistance Assays, what do the different banners mean (Qualified, Recommended, Control)?

- A recommended assay is the assay with the best strain coverage based on in silico prediction. In other words, recommended assays have the highest number of predicted on-targets and lowest number of predicted off-targets.
- A qualified assay has gone through R&D testing within the context of our main microbe detection applications, such as urinary tract, respiratory tract, and vaginal microbiota detection.
- A control banner is used exclusively for common control assays used in microbe detection applications.
I would like to order a control for my TaqMan Microbe Detection and Antibiotic Resistance Assay. Do you offer one? Do you have options for a custom control?

We have several controls for our pathogen-related content. To find a list of all available amplification controls, simply search in the Microbe Detection Search Tool (https://www.thermofisher.com/microbe-detection/taqman/query/) and click on the “Controls” option. Please contact Technical Support if you are unsure whether a particular control is compatible with your assay of interest. We also have options for creating a custom control, containing your specific targets of interest. You can find more information here: https://www.thermofisher.com/us/en/home/clinical/clinical-genomics/pathogen-detection-solutions/real-time-pcr-panel-controls.html
What protocol do you recommend using for the TaqMan Microbe Detection and Antibiotic Resistance Assays?

When using the assays in a single-tube format, we recommend following this general protocol (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0018536_TaqManMicrobialAssays_SingleTube_QR.pdf).
How do I find the TaqMan Microbe Detection and Antibiotic Resistance Assay I'm looking for and search for multiple targets?

There are several ways to search for TaqMan Microbe Detection and Antibiotic Resistance Assays. From the main Thermo Fisher Scientific website page (www.thermofisher.com), hover over the word “Popular” in the top navigation bar. From the menu that comes up, select “TaqMan Real-Time PCR Assays”. Under “The right TaqMan Assay is just a couple of clicks away”, from the TaqMan Assay dropdown, select “Microbe & ABR Detection”. In the search box, you can then enter your target organism and find your assay of interest.

You can also use these friendly URLs:
https://www.thermofisher.com/microbe-detection/taqman/query/
https://www.thermofisher.com/antibiotic-resistance/taqman/query/

To search for multiple targets, from the search results displayed above, click on the link in the upper right for “Search Multiple Targets”.
How do I find the TaqMan Microbe Detection and Antibiotic Resistance Assay I'm looking for?

There are several ways to search for TaqMan Microbe Detection and Antibiotic Resistance Assays. From the main Thermo Fisher Scientific website page (www.thermofisher.com), hover over the word “Popular” in the top navigation bar. From the menu that comes up, select “TaqMan Real-Time PCR Assays”. Under “The right TaqMan Assay is just a couple of clicks away”, from the TaqMan Assay dropdown, select “Microbe & ABR Detection”. In the search box, you can then enter your target organism and find your assay of interest.

You can also use these friendly URLs:
https://www.thermofisher.com/microbe-detection/taqman/query/
https://www.thermofisher.com/antibiotic-resistance/taqman/query/
Can I get the gene information for what the TaqMan Microbe Detection and Antibiotic Resistance Assay targets?

TaqMan Microbe Detection and Antibiotic Resistance Assays are provided with target strain information, not gene target information.
I'm searching for a TaqMan Microbe Detection and Antibiotic Resistance (ABR) assay on the website with the Assay ID but I can no longer find it, or I get a message that it has been discontinued. Can I still order the assay?

An assay will be marked as discontinued for the following reasons:
- The assay ID could be in an older format of Pa########_s1, for a “Pathogen” assay. We have converted these to a more organism-specific format (Ba/Vi/Fn/Pr for Bacteria/Virus/Fungi/Protozoa), and the assay sequences themselves are identical.
- There is a newer design that detects more strains (or fewer off-targets) than the current assay.

However, TaqMan Microbe Detection and Antibiotic Resistance Assays that provide a discontinued message on the website or in your shopping cart can still be ordered. Simply continue with the checkout process if you need to keep using this same assay. The discontinued message may be present because there is a new design. You can check for this and compare your options by doing the following:

Search for the target organism in the Microbe Assay Search Tool (https://www.thermofisher.com/microbe-detection/taqman/query/) (e.g., Hepatitis B virus, original assay ID Vi03453406_s1). The recommended assay will appear in the search result. You can review the target information under “Target Strains” and “Cross Reactivity” and compare with the previous assay. Depending on your workflow, (for example, starting a new study) you may consider switching to the new assay.
I have been told that the TaqMan Microbe Detection and Antibiotic Resistance (ABR) assay I used to order is discontinued. What does that mean?

There are two main reasons why an assay will be marked as discontinued:

- The assay ID could be in an older format of Pa########_s1, for a “Pathogen” assay. We have converted these to a more organism-specific format (Ba/Vi/Fn/Pr for Bacteria/Virus/Fungi/Protozoa), and the assay sequences themselves are identical.
- There is a newer design that detects more strains (or fewer off-targets) than the current assay.
Why do some TaqMan Microbe Detection and Antibiotic Resistance (ABR) assays appear to have multiple IDs? For example, is Pa03453406_s1 the same as Ba03453406_s1? Which one should I order?

The letter designation in the assay ID is intended for species identification. For our TaqMan Microbe Detection and Antibiotic Resistance Assays, this represents the organism (Ba = Bacteria, Vi = Virus, Fn = Fungi, Pr = Protozoa). When these assays were first released, the assay ID used the all-encompassing format of “Pa” for Pathogen. We have since duplicated all the “Pa” assay IDs into their respective organism-specific format. The assays themselves are exactly the same (i.e., no change in the primer and probe sequence). We encourage you to switch to the Ba/Vi/Fn/Pr format, as these will be displayed more easily on our website using our Microbe Assay Search Tool ((https://www.thermofisher.com/microbe-detection/taqman/query/)).