Reverse Transcription Products
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Thermo Scientific™ Maxima H Minus First Strand cDNA Synthesis Kit
Thermo Scientific Maxima H Minus First Strand cDNA Synthesis Kit, available with or without dsDNase, is a complete system for highly efficient synthesis of first strand cDNA.
| GC-Rich PCR Performance | High |
|---|---|
| Vitesse de réaction | 30 min. |
| Format de réaction | Separate components |
| Type de produit final | First-Strand cDNA |
| Activité de la ribonucléase H | Reduced |
| Conditions d’expédition | Dry Ice |
| Température de réaction optimale | 50°C to 55°C |
| Type de réactif | Reverse Transcription |
| Format | Kit |
| Quantité | Each |
| Technique | Reverse Transcription |
| Matériau de départ | RNA |
| Taille (produit final) | Up to 20 kb |
| Transcriptase inverse | Maxima H Minus |
Invitrogen™ SuperScript IV UniPrime One-Step RT-PCR System
Invitrogen SuperScript IV UniPrime One-Step RT-PCR System is a convenient one-step formulation for performing fast cDNA synthesis and PCR amplification in a single tube.
| Capacité multiplex | Yes |
|---|---|
| GC-Rich PCR Performance | High |
| Température de réaction optimale | 50°C to 65°C |
| Méthode de détection | Gel Electrophoresis |
| Type de réactif | Reverse Transcription |
| Format | 1-step RT-PCR Kit |
| Format de réaction | Master Mix |
| Hot Start | Built-in Hot Start |
| Méthode PCR | 1-step RT-PCR |
| Technique | 1-step RT-PCR |
| Matériau de départ | RNA |
| Taille (produit final) | Up to 13 kb |
| Transcriptase inverse | SuperScript IV |
Invitrogen™ SuperScript™ II Reverse Transcriptase
Invitrogen SuperScript II Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) with reduced RNase H activity and increased thermal stability compared to wild-type MMLV RT.
| GC-Rich PCR Performance | High |
|---|---|
| Vitesse de réaction | 50 min. |
| Format de réaction | Separate components |
| Type de produit final | First-Strand cDNA |
| Activité de la ribonucléase H | Reduced |
| Concentration | 200 U/μL |
| Conditions d’expédition | Wet Ice |
| Température de réaction optimale | 42°C |
| Type de réactif | Reverse Transcription |
| Format | Stand-alone Enzyme |
| Technique | Reverse Transcription |
| Matériau de départ | RNA |
| Taille (produit final) | Up to 12.3 kb |
| Transcriptase inverse | SuperScript II |
Invitrogen™ SuperScript™ III First-Strand Synthesis System
The SuperScript III First-Strand Synthesis System for RT-PCR is optimized to synthesize first-strand cDNA from purified poly(A)+ or total RNA.
Invitrogen™ SuperScript™ IV First-Strand Synthesis System
The SuperScript IV First-Strand Synthesis System for RT-PCR, available with or without ezDNase, is optimized for synthesis of first-strand cDNA from purified poly(A)+ or total RNA.
| GC-Rich PCR Performance | High |
|---|---|
| Température de réaction optimale | 50°C |
| Type de réactif | Reverse Transcription |
| Vitesse de réaction | 10 min. |
| Format | Kit |
| Format de réaction | Separate components |
| Type de produit final | First-Strand cDNA |
| Quantité | Each |
| Technique | Reverse Transcription |
| Matériau de départ | RNA |
| Taille (produit final) | Up to 12.3 kb |
| Transcriptase inverse | SuperScript IV |
Invitrogen™ SuperScript™ III Reverse Transcriptase
Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) created by the introduction of several mutations for reduced RNase H activity, increased half-life, and improved thermal stability.
| GC-Rich PCR Performance | High |
|---|---|
| Vitesse de réaction | 30 to 50 min. |
| Format de réaction | Separate components |
| Type de produit final | First-Strand cDNA |
| Activité de la ribonucléase H | Reduced |
| Concentration | 200 U/μL |
| Conditions d’expédition | Dry Ice |
| Température de réaction optimale | 50°C |
| Type de réactif | Reverse Transcription |
| Format | Stand-alone Enzyme |
| Technique | Reverse Transcription |
| Matériau de départ | RNA |
| Taille (produit final) | Up to 12.3 kb |
| Transcriptase inverse | SuperScript III |
Invitrogen™ SuperScript™ IV Reverse Transcriptase
SuperScript IV Reverse Transcriptase (RT) is a proprietary MMLV mutant with superior robustness and reliability in RT reactions.
| GC-Rich PCR Performance | High |
|---|---|
| Vitesse de réaction | 10 min. |
| Format de réaction | Separate components |
| Type de produit final | First-Strand cDNA |
| Activité de la ribonucléase H | Reduced |
| Concentration | 200 U/μL |
| Conditions d’expédition | Dry Ice |
| Température de réaction optimale | 50°C |
| Type de réactif | Reverse Transcription |
| Format | Stand-alone Enzyme |
| Technique | Reverse Transcription |
| Matériau de départ | RNA |
| Taille (produit final) | Up to 12.3 kb |
| Transcriptase inverse | SuperScript IV |
Thermo Scientific™ Maxima H Minus Reverse Transcriptase (200 U/μL)
Thermo Scientific Maxima H Minus Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity but lacks RNase H activity due to mutation in RNase H domain of M-MuLV RT.
| GC-Rich PCR Performance | High |
|---|---|
| Vitesse de réaction | 15 to 30 min. |
| Format de réaction | Separate components |
| Type de produit final | First-Strand cDNA |
| Activité de la ribonucléase H | Reduced |
| Concentration | 200 U/μL |
| Conditions d’expédition | Dry Ice |
| Température de réaction optimale | 50°C to 55°C |
| Type de réactif | Reverse Transcription |
| Format | Stand-alone Enzyme |
| Technique | Reverse Transcription |
| Matériau de départ | RNA |
| Taille (produit final) | Up to 20 kb |
| Transcriptase inverse | Maxima H Minus |
Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit
The High Capacity cDNA Reverse Transcription Kit, available with or without RNase Inhibitor, contains all components necessary for the quantitative conversion of up to 2 μg of total RNA into single-stranded cDNA in a single 20 μL reaction.
| Type de réactif | Reverse Transcription |
|---|---|
| Vitesse de réaction | 120 min. |
| Format | Kit |
| Format de réaction | Separate components |
| Type de produit final | First-Strand cDNA |
| Quantité | 1 Ea. |
| Activité de la ribonucléase H | Yes |
| Technique | Reverse Transcription |
| Concentration | 50 U/μL |
| Matériau de départ | RNA |
| Conditions d’expédition | Dry Ice |
| Transcriptase inverse | MultiScribe |
Thermo Scientific™ RevertAid RT Reverse Transcription Kit
The Thermo Scientific RevertAid RT Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates.
Invitrogen™ SuperScript™ III First-Strand Synthesis SuperMix
The SuperScript III First-Strand Synthesis SuperMix is an optimized formulation for first-strand cDNA synthesis from purified poly(A)+ or total RNA. RNA targets from 100 bp to >12 kb can be detected with this system.
Invitrogen™ SuperScript™ III CellsDirect™ cDNA Synthesis Kit
The SuperScript III CellsDirect cDNA Synthesis Kit is optimized for synthesis of first-strand cDNA directly from a mammalian cell lysate without first isolating the RNA.
| GC-Rich PCR Performance | High |
|---|---|
| Température de réaction optimale | 50°C |
| Type de réactif | Reverse Transcription |
| Vitesse de réaction | 50 min. |
| Format | Kit |
| Format de réaction | Separate components |
| Type de produit final | First-Strand cDNA |
| Activité de la ribonucléase H | Reduced |
| Technique | Reverse Transcription |
| Matériau de départ | Cells |
| Taille (produit final) | Up to 4.5 kb |
| Transcriptase inverse | SuperScript III |
| À utiliser avec (application) | Real Time PCR (qPCR) |
Applied Biosystems™ TaqMan™ Reverse Transcription Reagents
TaqMan Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)16, and reverse primers are included for cDNA synthesis flexibility.
| Température de réaction optimale | 42°C |
|---|---|
| Type de réactif | Reverse Transcription |
| Format | Kit |
| Format de réaction | Separate components |
| Type de produit final | First-Strand cDNA |
| Activité de la ribonucléase H | Yes |
| Technique | Reverse Transcription |
| Concentration | 50 U/μL |
| Matériau de départ | RNA |
| Taille (produit final) | Up to 5 kb |
| Conditions d’expédition | Dry Ice |
| Transcriptase inverse | MultiScribe |
| À utiliser avec (application) | Real Time PCR (qPCR) |
Invitrogen™ SuperScript™ Double-Stranded cDNA Synthesis Kit
Maximize the yield of full-length cDNA, as well as that of overall cDNA synthesis
| Température de réaction optimale | 42°C |
|---|---|
| Format | Kit |
| Méthode d’étiquetage | Direct Labeling |
| Type de produit | cDNA Synthesis Kit |
| Gamme de produits | SuperScript |
| Type d’échantillon | RNA |
| Type de produit final | cDNA (Double-Stranded) |
| Conditions d’expédition | Wet Ice |
| Transcriptase inverse | SuperScript™ II |
| À utiliser avec (application) | Microarray Analysis |
Invitrogen™ SuperScript™ VILO™ Master Mix
SuperScript VILO Master Mix is a first-strand cDNA synthesis master mix for two-step RT-qPCR applications.
| GC-Rich PCR Performance | High |
|---|---|
| Méthode de détection | qPCR |
| Vitesse de réaction | 60 min. |
| Format de réaction | Master Mix |
| Type de produit final | First-Strand cDNA |
| Activité de la ribonucléase H | Reduced |
| Concentration | 5X |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | Real Time PCR (qPCR) |
| Température de réaction optimale | 42°C |
| Type de réactif | Reverse Transcription |
| Format | Master Mix |
| Technique | Reverse Transcription |
| Matériau de départ | RNA |
| Taille (produit final) | Up to 10 kb |
| Transcriptase inverse | SuperScript III |