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Invitrogen™ SuperScript™ IV CellsDirect™ Lysis Reagents
SuperScript™ IV CellsDirect Lysis Reagents are optimized for synthesizing first-strand cDNA directly from a mammalian cell lysate without first isolating RNA.
Supplier: Invitrogen™ 11750550
Description
The SuperScript™ IV CellsDirect Lysis Reagents kit includes lysis solution, stop solution, and lysis enhancer. These reagents are part of the SuperScript™ IV CellsDirect cDNA Synthesis Kit and are made available separately here for use when processing a large number of samples. See the SuperScript™ IV CellsDirect cDNA Synthesis Kit manual for protocol recommendations.
- Ready-to-use pre-mixed cell lysis solution compatible with a variety of cell-types
- Optimized performance for 1–10,000 cells per sample; results equivalent to those from purified RNA
Specifications
• SuperScript IV CellsDirect Lysis Solution (9 mL) • SuperScript IV CellsDirect Stop Solution (1.25 mL) • Lysis Enhancer (250 µL) Store at –20°C. |
|
500 Reactions | |
Master Mix | |
Cells | |
SuperScript IV CellsDirect cDNA Synthesis Kit | |
Dry Ice |
Lysis Reagent | |
RT-PCR, RT-qPCR | |
Reverse Transcription | |
Reverse Transcription | |
SuperScript IV | |
RT-PCR (Endpoint), Real-Time PCR |