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  7. Invitrogen™ Second Strand cDNA Synthesis Kit

Invitrogen™ Second Strand cDNA Synthesis Kit

Numéro de catalogue. A48571
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No. of Reactions:
200 Reactions
50 Reactions
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Numéro de catalogue. No. of Reactions
A48571 200 Reactions
A48570 50 Reactions
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Numéro de catalogue. A48571 Fournisseur Invitrogen™ Code fournisseur A48571
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Description

Invitrogen™ Second Strand cDNA Synthesis Kit is design to produce double-stranded cDNA using first strand reaction without the need for intermediate organic extraction or ethanol precipitation steps. This convenient single-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains premixed components to reduce the number of pipetting steps necessary to complete the procedure.

InvitrogenTM Second Strand cDNA Synthesis Kit is design to produce double-stranded cDNA using first strand reaction without the need for intermediate organic extraction or ethanol precipitation steps. This convenient single-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains premixed components to reduce the number of pipetting steps necessary to complete the procedure.

Features of the Second Strand cDNA Synthesis Kit include:

  • Efficient synthesis of full-length double-stranded cDNA
  • Fast—procedure completed in 1 hour
  • Convenient—pre-mixed components
  • Complete—includes residual RNA removal reagents

Applications

  • Full-length double-stranded blunt-end cDNA synthesis for cloning
  • Double-stranded cDNA library construction

Spécifications

Content And Storage Contents:
• Second Strand Enzyme Mix, 4 x 1 mL
• 5X Second Strand Reaction Mix, 4 x 250 μL
• 0.5 M EDTA, pH 8.0, 4 x 1 mL
• RNase I, 10 U/μL, 4 x 500 μL
• Water, nuclease-free, 12 x 1.25 mL

Storage: -15 to -25°C
Format 200 reactions
Product Type cDNA Synthesis Kit
Quantity 200 reactions
For Use With (Application) Cloning
Final Product Type Double Stranded cDNA
No. of Reactions 200 Reactions
Reaction Format Separate Components