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Invitrogen™ SuperScript™ III First-Strand Synthesis SuperMix
Optimized SuperMix formulation for first-strand cDNA synthesis from purified poly(A)+ or total RNA
Supplier: Invitrogen™ 18080400
Includes: 2X Reaction Mix (500μL); SuperScript III Enzyme Mix (100μL); Oligo(dT)20 (50μL total provided at 50μM); Random hexamers (50μL total provided at 50ng/μL); Annealing buffer (50μL)
Description
RNA targets from 100 bp to >12kb can be detected with this system. The amount of starting material can vary from 0.1pg to 5μg of total RNA.
- Kit includes SuperScript™ III/RNaseOUT™ Enzyme Mix, 2X First-Strand Reaction Mix, and Annealing Buffer
- SuperScript™ III Reverse Transcriptase, included in the Enzyme Mix, is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability
- Enzyme can be used to synthesize cDNA at a temperature range of 45° to 55°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases
- Because SuperScript™ III RT is not significantly inhibited by ribosomal and transfer RNA, it can be used to synthesize cDNA from total RNA
- RNaseOUT™ Recombinant RNase Inhibitor is included in the Enzyme Mix to safeguard against degradation of target RNA due to ribonuclease contamination
- 2X First-Strand Reaction Mix includes 10mM MgCl2 and 1mM of each dNTP in a buffer formulation that has been optimized for first-strand synthesis of cDNA
- Annealing Buffer is used in the initial template-primer annealing step
- Separate tubes of oligo(dT)20 and random hexamers are also provided. cDNA synthesis can be performed using either total RNA or poly(A)+-selected RNA primed with oligo(dT), random primers, or a gene-specific primer
- Optimal reaction temperature: 50°C
- Enough material provided for 50 reactions (20μL each)
PCR & Real-Time PCR, Reverse Transcription
Order Info
Shipping Condition: Dry ice
Specifications
• 2X Reaction Mix, 500 μL |
|
First-Strand cDNA | |
High | |
RT-PCR, RT-qPCR | |
50 min. | |
RNA | |
20 μL | |
Random Primers, Oligo dT Primers | |
Dry Ice |
Primer-Probe | |
First-Strand Synthesis Super Mix | |
50 Reactions | |
Master Mix | |
Reverse Transcription | |
Reverse Transcription | |
50°C | |
SuperScript III | |
Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR) |
For Research Use Only. Not for use in diagnostic procedures.