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Invitrogen™ SuperScript™ III First-Strand Synthesis SuperMix DFS Item

Optimized SuperMix formulation for first-strand cDNA synthesis from purified poly(A)+ or total RNA

Supplier:  Invitrogen™ 18080400

Catalog No. 18080400



Includes: 2X Reaction Mix (500μL); SuperScript III Enzyme Mix (100μL); Oligo(dT)20 (50μL total provided at 50μM); Random hexamers (50μL total provided at 50ng/μL); Annealing buffer (50μL)

Description

Description

RNA targets from 100 bp to >12kb can be detected with this system. The amount of starting material can vary from 0.1pg to 5μg of total RNA.

  • Kit includes SuperScript™ III/RNaseOUT™ Enzyme Mix, 2X First-Strand Reaction Mix, and Annealing Buffer
  • SuperScript™ III Reverse Transcriptase, included in the Enzyme Mix, is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability
  • Enzyme can be used to synthesize cDNA at a temperature range of 45° to 55°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases
  • Because SuperScript™ III RT is not significantly inhibited by ribosomal and transfer RNA, it can be used to synthesize cDNA from total RNA
  • RNaseOUT™ Recombinant RNase Inhibitor is included in the Enzyme Mix to safeguard against degradation of target RNA due to ribonuclease contamination
  • 2X First-Strand Reaction Mix includes 10mM MgCl2 and 1mM of each dNTP in a buffer formulation that has been optimized for first-strand synthesis of cDNA
  • Annealing Buffer is used in the initial template-primer annealing step
  • Separate tubes of oligo(dT)20 and random hexamers are also provided. cDNA synthesis can be performed using either total RNA or poly(A)+-selected RNA primed with oligo(dT), random primers, or a gene-specific primer

  • Optimal reaction temperature: 50°C
  • Enough material provided for 50 reactions (20μL each)

PCR & Real-Time PCR, Reverse Transcription

Order Info

Shipping Condition: Dry ice

Specifications

Specifications

• 2X Reaction Mix, 500 μL
• SuperScript™ III Enzyme Mix, 100 μL
• Oligo(dT)20, 50 μL total (50 μM)
• Random hexamers, 50 μL (50 ng/μL)
• Annealing buffer, 50 μL

Store at –20°C.

First-Strand cDNA
High
RT-PCR, RT-qPCR
50 min.
RNA
20 μL
Random Primers, Oligo dT Primers
Dry Ice
Primer-Probe
First-Strand Synthesis Super Mix
50 Reactions
Master Mix
Reverse Transcription
Reverse Transcription
50°C
SuperScript III
Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR)
SDS
Documents

Documents

Product Certifications
Promotions

Promotions

For Research Use Only. Not for use in diagnostic procedures.