Protein Modification Reagents

MilliporeSigma™ Proteinase K Solution, 600mAU/mL

Efficient removal of proteins from nucleic acid solutions

MilliporeSigma™ Aminopeptidase N, Rat

Levels of aminopeptidase N are elevated in cancer subjects and correlate with tumor load, and thus, enzyme is believed to play role in angiogenesis

MilliporeSigma™ Aminopeptidase N (CD13), Human Serum

Prepared from human serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV

MilliporeSigma™ Calbiochem™ TPEN

Lipid-soluble heavy metal chelator that crosses artificial and natural membranes

Thermo Scientific™ DTNB (Ellman's Reagent) (5,5-dithio-bis-(2-nitrobenzoic acid)

Measure peptide cysteines or other free, reduced sulfhydryls (-SH groups) in solution using this thiol-reactive, colored compound (Amax 412nm).

Thermo Scientific™ Pierce™ SATP (N-succinimidyl-S-acetylthiopropionate)

Short (4.1A), amine-reactive (NHS ester) sulfhydryl-addition reagent; creates stable (protected yet exposable) -SH group for protein crosslinking strategies.

MilliporeSigma™ Calbiochem™ DM-NITROPHEN™ Reagent, Tetrasodium Salt

Caged Ca2+ chelator that undergoes a major and rapid decrease in Ca2+-binding affinity upon photolysis

Invitrogen™ DTT, 1M  

DTT, 1M

Thermo Scientific™ Trypsin, TPCK Treated

Thermo Scientific Pierce TPCK Trypsin is a serine endoprotease that is applicable to amino acid analysis and protein sequencing, mapping and structural studies; the immobilized form allows sample separation after treatment.

Thermo Scientific™ Pierce™ SATA (N-succinimidyl S-acetylthioacetate)

Short (2.8A), amine-reactive (NHS ester) sulfhydryl-addition reagent; creates stable (protected yet exposable) -SH group for protein crosslinking strategies.

Thermo Scientific™ Pierce™ TCEP-HCl, No-Weigh™ Format

Reduce protein and peptide disulfide bonds using this thiol-free, pure, crystalline tris (2-carboxyethyl) phosphine hydrochloride, TCEP (CAS 5961-85-3).

Thermo Scientific™ Pierce™ DTT (Dithiothreitol), No-Weigh™ Format

Thermo Scientific™ Pierce™ DTT (Dithiothreitol) No-Weigh™ Format is designed for convenient preparation of stocks that will be added to protein samples for effective reduction of disulfide bonds.

Thermo Scientific™ WELQut Protease (5 U/μL)

Thermo Scientific™ WELQut Protease is a highly specific serine protease cleaving outside the WELQX recognition motif to remove N-terminal protein fusion tags.

2-Mercaptoethanol, For HPLC derivatization (14.3 M), ≥99.0% (GC), MilliporeSigma™ Supelco™

MilliporeSigma™ Calbiochem™ Cleland’s Reagent

Protective agent for SH groups