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MilliporeSigma™ Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes

Catalog No. 3247155UG
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5 μg
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3247155UG 5 μg
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Catalog No. 3247155UG Supplier MilliporeSigma™ Supplier No. 3247155UG
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Description

Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins

  • Native endoproteinase Lys-C from Lysobacter enzymogenes
  • Serine protease that specifically hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine in peptides and proteins
  • Inhibited by aprotinin, DFP, leupeptin, and TLCK
  • Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis

Specifications

Concentration Suggested working concentration: 1:20 to 1:100 (protease:protein by weight)
Formulation Lyophilized from 50mM HEPES, 10mM EDTA, 5mg/mL raffinose, pH 8.0
pH Range Optimum 8.5 to 8.8
Purity ≥90% by SDS-PAGE
CAS 72561-05-8
Content And Storage 2°C to 8°C
Form Lyophilized
Quantity 5 μg
Solubility Water
Product Type Endoproteinase