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MilliporeSigma™ Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes

Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins

Supplier: MilliporeSigma™ 3247155UG

Catalog No. 3247155UG

Quantity

Description

Native endoproteinase Lys-C from Lysobacter enzymogenes
Serine protease that specifically hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine in peptides and proteins
Inhibited by aprotinin, DFP, leupeptin, and TLCK
Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis

Specifications

Concentration	Suggested working concentration: 1:20 to 1:100 (protease:protein by weight)
pH Range	Optimum 8.5 to 8.8
CAS	72561-05-8
Form	Lyophilized
Solubility	Water

Formulation	Lyophilized from 50mM HEPES, 10mM EDTA, 5mg/mL raffinose, pH 8.0
Purity	≥90% by SDS-PAGE
Content And Storage	2°C to 8°C
Quantity	5 μg
Product Type	Endoproteinase

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