Protein Modification Reagents

Thermo Scientific™ Proteinase K Powder (≥30 U/mg)

Cleave at the carboxyl-side of aliphatic, aromatic or hydrophobic residues and digest-inactivate DNase and RNase in nucleic acid purification using this protease.

Thermo Scientific™ Trypsin, TPCK Treated

Thermo Scientific Pierce TPCK Trypsin is a serine endoprotease that is applicable to amino acid analysis and protein sequencing, mapping and structural studies; the immobilized form allows sample separation after treatment.

Invitrogen™ DTT, 1M  

DTT, 1M

Thermo Scientific™ Urea

Pure, crystalline urea (CH4N2O), CAS 57-13-6; denaturant with low UV-absorptivity for protein biology methods (elution, solubilization, refolding, storage).

MilliporeSigma™ Calbiochem™ SPUTOLYSIN™ Reagent

Intended for isolation of epithelial cells, pathogenic or saprophytic bacteria, fungi, and yeasts from sputum

Thermo Scientific™ Pierce™ Alkylating Reagents

Alkylating reagents provided in single-use, pre-weighed formats to streamline protein preparation workflows.

MilliporeSigma™ ALCALASE™ Enzyme, Bacillus licheniformis

Suitable for hydrolysis of proteins

MilliporeSigma™ Novagen™ Overnight Express™ Autoinduction System 1

Induces protein expression without monitoring cell density.

Gibco™ PNGase F Glycan Cleavage Kit  

PNGase F Glycan Cleavage Kit includes all components necessary to perform the enzymatic removal of almost all N-linked oligosaccharides from glycoproteins.

Invitrogen™ Dithiothreitol (DTT)  

Dithiothreitol (DTT)

Invitrogen™ Tris-(2-Carboxyethyl)phosphine, Hydrochloride (TCEP)

Tris-(2-Carboxyethyl)phosphine, Hydrochloride (TCEP)

Thermo Scientific™ Pierce™ SATA (N-succinimidyl S-acetylthioacetate)

Short (2.8A), amine-reactive (NHS ester) sulfhydryl-addition reagent; creates stable (protected yet exposable) -SH group for protein crosslinking strategies.

Thermo Scientific™ Immobilized Papain (Agarose Resin)

Generate and purify Fab fragments from antibodies with cysteine-endopeptidase papain immobilized onto beaded agarose resin.

Thermo Scientific™ Pierce™ Cysteine-HCl

Make sulfhydryl assay standards or perform protein refolding experiments using this pure, crystalline cysteine hydrochloride (Cys-HCl).

2-Mercaptoethanol, For HPLC derivatization (14.3 M), ≥99.0% (GC), MilliporeSigma™ Supelco™