Proteomics and Immunoassays

MilliporeSigma™ Amicon™ Ultra Centrifugal Filter Units

Achieve both high sample recovery and fast sample processing. Amicon™ Ultra Centrifugal Filter Units feature regenerated cellulose membranes that are ideal for protein and nucleic acid purification, concentration and desalting. Available in several diameters and molecular weight limits.

MilliporeSigma™ BioPak™ Disposable Ultrafilter

Nuclease-free, pyrogen-free water

Thermo Scientific™ Pierce™ BCA Protein Assay Kit

Green-to-blue (A562nm), precise, detergent-compatible assay reagent to measure total protein concentration vs. protein standard.

Thermo Scientific™ Pierce™ Bovine Serum Albumin Standard Ampules, 2 mg/mL

High-quality, calibrated BSA solutions (2mg/mL) to serially dilute to create protein assay standard curves to accurately measure protein concentration.

Acrylamide: Bis-Acrylamide 29:1 (40% Solution/Electrophoresis), Fisher BioReagents

CAS: 79-06-1 Molecular Formula: C3H5NO Molecular Weight (g/mol): 71.079 InChI Key: HRPVXLWXLXDGHG-UHFFFAOYSA-N PubChem CID: 6579 ChEBI: CHEBI:28619 IUPAC Name: prop-2-enamide SMILES: C=CC(=O)N

Thermo Scientific™ Nalgene™ Analytical Test Filter Funnels

Perform microbiological QC testing and analysis of water, food/beverage, raw material and finished product with these economical sterile disposable funnels.

Thermo Scientific™ Pierce™ Coomassie (Bradford) Protein Assay Kit

Brown-to-purple (A595nm), ready-to-use, classic reducing agent-compatible assay reagent to measure total protein concentration vs. protein standard.

Thermo Scientific™ SuperSignal™ West Femto Maximum Sensitivity Substrate

Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.

GE Healthcare Amersham™ ECL™ Prime Western Blotting Detection Reagent

Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity.  GE Healthcare Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity.

Thermo Scientific™ Pierce™ 16% Formaldehyde (w/v), Methanol-free

Vials of high-quality, methanol-free formaldehyde, a general IHC fixative and reversible amine-crosslinking agent for protein and nucleic acid samples.

Corning™ Laminin, Mouse

Promotes cell adhesion, migration, growth, and differentiation, including neurite outgrowth

MilliporeSigma™ Immobilon™ Western Chemiluminescent HRP Substrate (ECL)

Provides high sensitivity over a broad detection range at an economical price

Thermo Scientific™ HisPur™ Ni-NTA Spin Plates

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

Thermo Scientific™ Pierce™ ECL Western Blotting Substrate

Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

GE Healthcare Amersham™ ECL™ Western Blotting Detection Reagents

Use for all routine Western blotting. GE Healthcare Life Sciences™ Amersham™ ECL™ Western Blotting Detection Reagents are the world’s first commercially available chemiluminescent detection reagents and still one of the most widely used.

MilliporeSigma™ Luminata™ Western HRP Chemiluminescence Substrates

Premixed, ready-to-use chemiluminescent reagents for detection of HRP-based Westerns

Thermo Scientific™ Micro BCA™ Protein Assay Kit

Special 3-component version of our popular BCA Reagents to measure total protein concentration of dilute protein solutions (0.5 to 20 micrograms/mL).

Acrylamide: Bis-Acrylamide 37.5:1 (40% Solution/Electrophoresis), Fisher BioReagents

CAS: 79-06-1 Molecular Formula: C3H5NO Molecular Weight (g/mol): 71.079 InChI Key: HRPVXLWXLXDGHG-UHFFFAOYSA-N PubChem CID: 6579 ChEBI: CHEBI:28619 IUPAC Name: prop-2-enamide SMILES: C=CC(=O)N

Thermo Scientific™ RIPA Lysis and Extraction Buffer

Lyse cultured mammalian cells with this high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent.

Thermo Scientific™ Pierce™ Modified Lowry Protein Assay Kit

Clear-to-purple (A750nm), 2-component, folin phenol- and copper-based reagent system to measure total protein concentration vs. protein standard.

Thermo Scientific™ T-PER™ Tissue Protein Extraction Reagent

Extract total protein from tissue samples using this mild cell lysis and extraction reagent developed specifically for tissues.

Thermo Scientific™ ELISA Reagent Reservoir

Rapidly dispense reagents with multi-channel pipettors using these sterile, sturdy and disposable reservoirs molded from high-impact polystyrene.

Thermo Scientific™ Lysozyme Solution (50 mg/mL)

Increase protein or nucleic acid extraction efficiency with this enzyme that is characterized by the ability to break down the bacterial cell wall.

Sartorius™ Vivaspin™ 20 Centrifugal Concentrator

Offers increased volume flexibility and performance. Vivaspin™ 20 Centrifugal Concentrator handles up to 20mL in swing bucket rotors and 14mL in fixed angle rotors accepting 50mL centrifuge tubes. Features twin vertical membranes for unparalleled filtration speed and >100-fold concentration.

Thermo Scientific™ Shandon™ TPX Single Sample Chamber, Caps, and Filter Cards

For use with disposable white filter cards for sample volumes up to 0.5mL and for use with disposable brown filter cards for sample volumes of 0.4mL or less

Thermo Scientific™ Pierce™ EDC, No-Weigh™ Format

Immobilize or conjugate proteins using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (CAS 25952-53-8), a water-soluble, carboxyl-to-amine crosslinker.

GE Healthcare Sephaorose™ Fast Flow Anion Exchange Media: Q

Use for preparative protein separations in both research and industrial applications. GE Healthcare Q Sepharose™ Fast Flow Chromatography Media is a strong anion exchanger based on the well established Sepharose Fast Flow ion exchange platform.

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