Reverse Transcription Products
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Thermo Scientific™ RevertAid Reverse Transcriptase (200 U/μL)
Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties.
| Shipping Condition | Dry Ice |
|---|---|
| Content And Storage | • RevertAid Reverse Transcriptase (200 U/μL) |
| Reverse Transcriptase | RevertAid |
| Final Product Type | First-Strand cDNA |
| Concentration | 200 U/μL |
| Format | Stand-alone Enzyme |
| Size (Final Product) | Up to 13 kb |
| Reaction Format | Separate components |
| Reaction Speed | 60 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Yes |
| Optimal Reaction Temperature | 42°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq High Fidelity DNA Polymerase
The SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity is designed for convenient end-point detection and analysis of RNA molecules by one-step RT-PCR.
| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Reverse Transcriptase | SuperScript III |
| Final Product Type | PCR Amplified cDNA |
| PCR Method | 1-step RT-PCR |
| Detection Method | Gel Electrophoresis |
| Polymerase | Platinum Taq High Fidelity |
| Format | One-Step RT-PCR System |
| Size (Final Product) | Up to 10 kb |
| Reaction Format | Premixed Components |
| Reaction Speed | 30 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Reduced |
| Hot Start | Built-In Hot Start |
| Optimal Reaction Temperature | 50°C |
| Technique | 1-Step RT-PCR |
| Starting Material | RNA |
Thermo Scientific™ Verso cDNA Synthesis Kit
The Thermo Scientific Verso cDNA Synthesis Kit provides robust transcription of RNA to create a complete cDNA pool.
| Shipping Condition | Wet or Dry Ice |
|---|---|
| Format | Kit |
| Reverse Transcriptase | Verso |
| Size (Final Product) | Up to 11 kb |
| Reaction Format | Separate components |
| Final Product Type | First-Strand cDNA |
| Reaction Speed | 30 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Yes |
| Optimal Reaction Temperature | 42°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
| For Use With (Application) | Real Time PCR (qPCR),RT-PCR |
Invitrogen™ MultiScribe™ Reverse Transcriptase
MultiScribe Reverse Transcriptase is a recombinant moloney murine leukemia virus (rMoMuLV) reverse transcriptase optimized for TaqMan-based assays.
Invitrogen™ SuperScript IV UniPrime One-Step RT-PCR System
Invitrogen SuperScript IV UniPrime One-Step RT-PCR System is a convenient one-step formulation for performing fast cDNA synthesis and PCR amplification in a single tube.
| GC-Rich PCR Performance | High |
|---|---|
| Format | 1-step RT-PCR Kit |
| Reverse Transcriptase | SuperScript IV |
| Size (Final Product) | Up to 13 kb |
| Reaction Format | Master Mix |
| Reagent Type | Reverse Transcription |
| Hot Start | Built-in Hot Start |
| Optimal Reaction Temperature | 50°C to 65°C |
| Technique | 1-step RT-PCR |
| Starting Material | RNA |
| PCR Method | 1-step RT-PCR |
| Multiplex Capability | Yes |
| Detection Method | Gel Electrophoresis |
Invitrogen™ SuperScript™ One-Step RT-PCR System with Platinum™ Taq DNA Polymerase
SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the convenient end-point detection and analysis of RNA molecules by RT-PCR.
Invitrogen™ SuperScript™ IV VILO™ Master Mix
SuperScript IV VILO (SSIV VILO) Master Mix, available with or without ezDNase, is a reaction master mix designed for fast, sensitive, and reproducible cDNA synthesis in RT-qPCR applications.
| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Reverse Transcriptase | SuperScript IV |
| Final Product Type | First-Strand cDNA |
| Quantity | Each |
| Concentration | 5X |
| For Use With (Application) | Real Time PCR (qPCR) |
| Detection Method | qPCR |
| Format | Master Mix |
| Size (Final Product) | Up to 10 kb |
| Reaction Format | Master Mix |
| Reaction Speed | 10 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Reduced |
| Optimal Reaction Temperature | 50°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
Invitrogen™ Second Strand cDNA Synthesis Kit
Invitrogen™ Second Strand cDNA Synthesis Kit is design to produce double-stranded cDNA using first strand reaction without the need for intermediate organic extraction or ethanol precipitation steps. This convenient single-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains premixed components to reduce the number of pipetting steps necessary to complete the procedure.
| Reaction Format | Separate Components |
|---|---|
| Final Product Type | Double Stranded cDNA |
| Product Type | cDNA Synthesis Kit |
| For Use With (Application) | Cloning |
Thermo Scientific™ Maxima H Minus First Strand cDNA Synthesis Kit
Thermo Scientific Maxima H Minus First Strand cDNA Synthesis Kit, available with or without dsDNase, is a complete system for highly efficient synthesis of first strand cDNA.
| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Reverse Transcriptase | Maxima H Minus |
| Final Product Type | First-Strand cDNA |
| Quantity | Each |
| Format | Kit |
| Size (Final Product) | Up to 20 kb |
| Reaction Format | Separate components |
| Reaction Speed | 30 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Reduced |
| Optimal Reaction Temperature | 50°C to 55°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
Invitrogen™ SuperScript™ VILO™ Master Mix
SuperScript VILO Master Mix is a first-strand cDNA synthesis master mix for two-step RT-qPCR applications.
| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Reverse Transcriptase | SuperScript III |
| Final Product Type | First-Strand cDNA |
| Concentration | 5X |
| For Use With (Application) | Real Time PCR (qPCR) |
| Detection Method | qPCR |
| Format | Master Mix |
| Size (Final Product) | Up to 10 kb |
| Reaction Format | Master Mix |
| Reaction Speed | 60 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Reduced |
| Optimal Reaction Temperature | 42°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
Invitrogen™ SuperScript™ IV Template Switching RT Master Mix
Invitrogen SuperScript IV Template-Switching RT Master Mix is designed for high template switching efficiency in cDNA synthesis reactions.
| GC-Rich PCR Performance | High |
|---|---|
| Format | Master Mix |
| Final Product Type | First-Strand cDNA |
| Reaction Speed | 30 min. |
| Reagent Type | Reverse Transcription |
| Technique | Template Switching Reverse Transcription |
| Concentration | 4X |
| Starting Material | Cells or RNA |
Invitrogen™ SuperScript™ III First-Strand Synthesis System
The SuperScript III First-Strand Synthesis System for RT-PCR is optimized to synthesize first-strand cDNA from purified poly(A)+ or total RNA.
Invitrogen™ SuperScript™ III CellsDirect™ cDNA Synthesis Kit
The SuperScript III CellsDirect cDNA Synthesis Kit is optimized for synthesis of first-strand cDNA directly from a mammalian cell lysate without first isolating the RNA.
| GC-Rich PCR Performance | High |
|---|---|
| Format | Kit |
| Reverse Transcriptase | SuperScript III |
| Size (Final Product) | Up to 4.5 kb |
| Reaction Format | Separate components |
| Final Product Type | First-Strand cDNA |
| Reaction Speed | 50 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Reduced |
| Optimal Reaction Temperature | 50°C |
| Technique | Reverse Transcription |
| Starting Material | Cells |
| For Use With (Application) | Real Time PCR (qPCR) |
Invitrogen™ SuperScript™ IV First-Strand Synthesis System
The SuperScript IV First-Strand Synthesis System for RT-PCR, available with or without ezDNase, is optimized for synthesis of first-strand cDNA from purified poly(A)+ or total RNA.
| GC-Rich PCR Performance | High |
|---|---|
| Format | Kit |
| Reverse Transcriptase | SuperScript IV |
| Size (Final Product) | Up to 12.3 kb |
| Reaction Format | Separate components |
| Final Product Type | First-Strand cDNA |
| Reaction Speed | 10 min. |
| Reagent Type | Reverse Transcription |
| Quantity | Each |
| Optimal Reaction Temperature | 50°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
Applied Biosystems™ High-Capacity RNA-to-cDNA™ Kit
The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR Green PCR Master Mix, and other PCR enzymes.
| Shipping Condition | Dry Ice |
|---|---|
| Format | Kit |
| Reverse Transcriptase | MultiScribe |
| Size (Final Product) | Up to 7 kb |
| Reaction Format | Premixed Components |
| Final Product Type | First-Strand cDNA |
| Reaction Speed | 30 to 60 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Yes |
| Optimal Reaction Temperature | 37°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
| For Use With (Application) | Real Time PCR (qPCR),RT-PCR |