Substrates

Invitrogen™ DQ™ Red BSA - Special Packaging  

Hydrolysis of substrate yields brightly fluorescent product

Invitrogen™ DQ™ Gelatin From Pig Skin, Fluorescein Conjugate - Special Packaging  

Analog of natural substrate

Invitrogen™ PED-A1 (N-((6-(2,4-DNP)Amino)Hexanoyl)-1-(BODIPY™ FL C5)-2-Hexyl-Sn-Glycero-3-Phosphoethanolamine) (Phospholipase A1 Selective Substrate)  

PED-A1-labeled phospholipid

Invitrogen™ Red/Green BODIPY™ PC-A2 (1-O-(6-BODIPY™ 558/568-Aminohexyl)-2-BODIPY™ FL C5-Sn-Glycero-3-Phosphocholine) (Ratiometric Phospholipase A2 Substrate)  

Measure results in microplate assays or using flow cytometry

Thermo Scientific Chemicals TMB QUICK, for ELISA, Liquid Substrate

Gibco™ CELLstart™ Substrate  

CELLstart™ Substrate

CCF2-AM  

CCF2-AM

Invitrogen™ CMAC, t-BOC-Leu-Met (7-Amino-4-Chloromethylcoumarin, t-BOC-L-Leucyl-L-Methionine amide)  

Cleavage produces blue-fluorescence

Invitrogen™ DQ™ Collagen, type I From Bovine Skin, Fluorescein Conjugate  

The fluorogenic DQ™ collagen can be used to directly monitor collagenase activity.

Invitrogen™ DDAO galactoside (9H-(1,3-Dichloro-9,9-Dimethylacridin-2-One-7-yl) β-D-Galactopyranoside)  

The galactosidase substrate, DDAO galactoside yields a hydrolysis product that can be excited with the 633 nm laser (excitation/emission maxima ∼645/660).

Invitrogen™ Fluorescein Di-β-D-Glucopyranoside (FDGlu)  

Cell permeant

Thermo Scientific™ CDK7/9tide Substrate  

A peptide substrate based upon the C-terminal tail of RNA Polymerase II that is a reported substrate for Cyclin Dependent Kinases (CDK7, CDK8 and CDK9).

Thermo Scientific™ ZIPtide Substrate  

A peptide substrate for the Ca/Calmodulin-dependent protein kinase (CaM) family including CAMK1 and DAPK1.

Thermo Scientific™ PI:PS Lipid Kinase Substrate  

Specifically optimized for use with the Class III PI3 kinase (PIK3C3 (hVPS34)) and Type III PI4 lipid kinases, this substrate can also be used as a general substrate for the Class I lipid kinases.

Thermo Scientific™ CHKtide  

A peptide substrate based upon CDC25C that is phosphorylated by CHEK1 and other kinases.