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Fluorescein-Poly GT

Catalog No. PV3610
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PV3610 1 mL
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Catalog No. PV3610 Supplier Thermo Fisher Scientific Supplier No. PV3610
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Description

Fluorescein-Poly GT

A general fluorescein peptide substrate consisting of a polymer of glutamate, and tyrosine that is used to monitor tyrosine kinase activity.

For use in combination with the LanthaScreen™ Tb-pY20 Antibody (PV3552) to evaluate inhibitors in medium to high throughput screening applications.

Specifications

Amino Acid Sequence Fluorescein- Poly Glu:Tyr (4:1)
Assay Entry Biochemical terbium phospho-specific activity
Content And Storage 30 μM Fluorescein Poly-GT peptide substrates in 10 mM HEPES, 137 mM NaCl, 2.7 mM KCl (HEPES buffered saline). Store at -20°C and protect from light.
Detection Method Fluorescence
Form Liquid
Quantity 1 mL
Substrate Fluorescein-Poly GT
Substrate Properties Peptide-Based Substrate
Substrate Type Kinase Substrate
Target Enzyme Tyrosine Kinases
Label or Dye FITC (Fluorescein)
Shipping Condition Dry Ice
Target Entry ABL1 G250E, CSF1R, CSK, EPHA5, KIT, PDGFRA T674I, PDGFRA V561D, PTK6, RET Y791F, SYK, TYRO3, ZAP70, FGFR1, FGFR2, BLK, EGFR, EPHB2, FGFR4, IGF1R, LYNA, MATK, MERTK, NTRK3, RET V804L, ROS1, TEK, TTK, FLT1, MUSK, SRCN1, TEC, ABL1 E255K, BMX, EPHA4, FYN, PTK2, PTK2B, RET, ABL1 T315I, EPHA3, EPHB4, FLT3, FLT4, INSR, LCK, YES1, EGFR L858R, ERBB4, KDR, MET, TNK2, ABL1, ABL2, AXL, FGFR3, FGFR3 K650E, FGR, HCK, KIT V654A, EPHA1, EPHA8, EPHB3, INSRR, LYNB, MST1R, NTRK1, PDGFRA D842V, SRMS, EGFR L861Q, EPHA2, FER, LTK, PDGFRA, PDGFRB, EGFR T790M, EPHA7, EPHB1, MET M1250T, FRK, ITK, FLT3 D835Y, SRC, ABL1 Y253F, BTK, DDR2, FES, KIT T670I, TXK, ERBB2, NTRK2
Validated Kinases ABL1, ABL2, AXL, BLK, BMX, BTK, CSF1R, CSK, DDR2, EGFR, EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA7, EPHA8, EPHB1, EPHB2, EPHB3, EPHB4, ERBB2, ERBB4, FER, FGFR1, FGFR2, FGFR3, FGFR4, FGR, FLT1, FLT3, FLT4, FRK, FYN, HCK, IGF1R, INSR, KDR, KIT, LCK, LTK, LYNA, LYNB, MATK, MERTK, MET, MST1R, MUSK, NTRK1, NTRK2, NTRK3, PDGFRA, PDGFRB, PTK2, PTK2B, PTK6, RET, ROS1, SRC, SRCN1, SRMS, SYK, TEC, TEK, TNK2, TTK, TYRO3, YES1, ZAP70
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For Research Use Only. Not for use in diagnostic procedures.