Invitrogen™ DDAO Phosphate (9H-(1,3-Dichloro-9,9-Dimethylacridin-2-One-7-yl) Phosphate, Diammonium Salt)

The phosphatase substrate DDAO phosphate [9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-yl) phosphate, diammonium salt] yields a hydrolysis product that can be excited with the 633nm laser (excitation/emission maxima approx. 645/660). Although the substrate itself is fluorescent (excitation/emission maxima approx. 460/610nm), the difference between the substrate excitation maximum and that of its hydrolysis product is over 200nm, allowing the two species to be easily distinguished.

Cell Analysis, Drug Discovery and Development, Enzyme and Protein Activity Assays, Labeling Chemistry, Labeling QC and Calibration, Phosphatase Activity, Phosphatase Activity Assays, Phosphatase Biology, Proteins, Expression, Isolation and Analysis, Target and Lead Identification and Validation