Protein Modification Reagents

Chemical agents used to modify the amino acid side chains of proteins in order to alter their native charges, block or expose reactive binding sites, inactivate functional groups, and change functional groups to create targets for crosslinking and labeling.

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Thermo Scientific™ Proteinase K Powder (≥30 U/mg)

Cleave at the carboxyl-side of aliphatic, aromatic or hydrophobic residues and digest-inactivate DNase and RNase in nucleic acid purification using this protease.

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Thermo Scientific™ Bond-Breaker™ TCEP Solution, Neutral pH

Efficiently reduce protein disulfide bonds with this stable, odorless, 0.5M solution of TCEP reducing agent that is ideal for making reducing SDS-PAGE sample buffers.

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Thermo Scientific™ Trypsin, TPCK Treated

Thermo Scientific Pierce TPCK Trypsin is a serine endoprotease that is applicable to amino acid analysis and protein sequencing, mapping and structural studies; the immobilized form allows sample separation after treatment.

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Invitrogen™ DTT, 1M

DTT, 1M

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Thermo Scientific™ Urea

Pure, crystalline urea (CH4N2O), CAS 57-13-6; denaturant with low UV-absorptivity for protein biology methods (elution, solubilization, refolding, storage).

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MilliporeSigma™ Calbiochem™ SPUTOLYSIN™ Reagent

Intended for isolation of epithelial cells, pathogenic or saprophytic bacteria, fungi, and yeasts from sputum

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Thermo Scientific™ Pierce™ Traut's Reagent (2-iminothiolane)

2-Iminothiolane-HCl (CAS 4781-83-3); an amine-reactive thiolation reagent to cap protein primary amines with sulfhydryl groups for immediate crosslinking.

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Thermo Scientific™ Pierce™ Hydroxylamine-HCl

NH2OH (CAS 7803-49-8) to cleave Asn-Gly peptide bonds or deprotect sulfhydryls following S-acetylthioacetate modification with SATA reagent.

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Thermo Scientific™ MA(PEG)12 Methyl-PEG-Amine Compound

MA(PEG)n: methyl- and amine-capped polyethylene glycol compounds containing 4 to 24 PEG units; use for surface-modification and molecule-pegylation applications.

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Specifications

Content And Storage	Upon receipt store product at -20°C protected from moisture. Product is shipped at ambient temperature.
Product Type	Amine Compound
Form	Solid
Spacer	12 ethylene glycol units
Solubility	Water
Reactive Moiety	Amine, Primary Amine
Label Type	Polyethylene Glycol (PEG), Blocking Groups
Chemical Reactivity	Aldehyde

MilliporeSigma™ Calbiochem™ BAPTA/AM

Can be loaded into wide variety of cells, where it is hydrolyzed by cytosolic esterases and is trapped intracellularly as the active chelator BAPTA

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Specifications

Membrane Permeable	Y
Content And Storage	-20°C
CAS	126150-97-8
Product Type	Calbiochem BAPTA/AM
Form	Solid
Solubility	DMSO
Purity	≥90% by TLC
Synonym	1,2-bis(o-Aminophenoxy)ethane-N,N,Nʹ,Nʹ-tetraacetic Acid Tetra(acetoxymethyl) Ester