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Direct PCR Reagents and Kits

PCR products that allow for the amplification of DNA directly from unpurified source material. Includes various kits, master mixes, enzymes, and reagents.
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1

Thermo Scientific™ Phusion Blood Direct PCR Kit

Thermo Scientific Phusion Blood Direct PCR Kit is developed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR.

Shipping Condition Wet or Dry Ice
Polymerase Phusion Blood II DNA Polymerase
GC-Rich PCR Performance High
Reaction Format Separate Components
Product Type Direct PCR Kit
Sample Type Blood
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
Concentration 2 U/μL
Starting Material DNA
PCR Method Direct PCR
2

Thermo Scientific™ Phusion Human Specimen Direct PCR Kit

Thermo Scientific Phusion Human Specimen Direct PCR Kit delivers unprecedented convenience for DNA amplification by allowing PCR directly from unpurified human samples.

3

Invitrogen™ Cells-to-cDNA™ II Cell Lysis Buffer (10 ml Bottle)  DFS Item

The same lysis buffer found in the Invitrogen Cells-to-cDNA™ II Kit. Supplied in five 10 mL bottles.

4

Thermo Scientific™ Phusion Blood Direct PCR Master Mix

Thermo Scientific Phusion Blood Direct PCR Master Mix is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR.

Shipping Condition Wet or Dry Ice
GC-Rich PCR Performance High
Includes Label or Dye Yes
Overhang Blunt
Concentration 2X
For Use With (Application) Genotyping
PCR Method Direct PCR
Polymerase Phusion Blood II DNA Polymerase
Reaction Format SuperMix or Master Mix
Product Type Direct PCR Master Mix
Sample Type Blood
Reaction Speed Fast
Hot Start Built-In Hot Start
Starting Material DNA
5

Applied Biosystems™ SNaPshot™ Multiplex Kit  DFS Item

The SNaPshot™ Multiplex Kit is part of the SNaPshot™ Multiplex System. The SNaPshot™ Multiplex System primer extension-based method enables multiplexing up to 10 single nucleotide polymorphisms (SNPs).

Product Type Multiplex Kit
For Use With (Equipment) 3500 Series Genetic Analyzers, SeqStudio Flex Series Genetic Analyzers, SeqStudio Genetic Analyzer, 3730/3730xl Genetic Analyzers
Sample Type DNA
Technique SNP Genotyping (Fragment Analysis-Based), Primer Extension
Product Line SNaPshot
Detection Method Primer-probe
6

Invitrogen™ TaqMan™ PreAmp Cells-to-CT™ Kit  DFS Item
GREENER_CHOICE

The TaqMan PreAmp Cells-to-CT Kit makes it possible to perform gene expression analysis directly from limited or small numbers of cultured cells without RNA purification.

7

Applied Biosystems™ MagMAX™ Viral/Pathogen Binding Beads  DFS Item

These binding beads are included in the MagMAX Viral/Pathogen Nucleic Acid Isolation kits and MagMAX Microbiome Ultra Nucleic Acid Isolation Kit.

8

Thermo Scientific™ Phire Animal Tissue Direct PCR Kit (without sampling tools)

Thermo Scientific Phire Animal Tissue Direct PCR Kit has been developed for amplification of DNA directly from a wide variety of animal tissues including mice, fish, birds and insects.

9

Invitrogen™ PureLink™ PCR Micro Kit  DFS Item

PureLink PCR Micro Kits are ideal for purifying and concentrating DNA from low-yielding PCR or restriction digestion reactions.

Shipping Condition Room Temperature
Separation Range 100 bp to 12 kb
Content And Storage Kit includes columns, binding and wash buffers, recovery tubes, and the manual. Store at room temperature.
Starting Material Volume 125 μL
Isolation Technology Silica Spin Column
Elution Volume Max 20 μL
For Use With (Application) Cloning,Standard PCR
High-throughput Compatibility Not High-throughput Compatible (Manual)
Target DNA from Enzymatic Reactions (e.g. PCR)
Format Spin Column
Product Type PCR Micro Kit
Run Time 10 min.
Elution Volume Min 5 μL
Product Line PureLink
10

Invitrogen™ Cells-to-CT™ 1-Step TaqMan™ Kit  DFS Item
GREENER_CHOICE

Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step TaqMan™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.

GC-Rich PCR Performance High
Format Tube
Product Type 1-Step qPCR kit
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type Mammalian Cells
Reaction Speed 7 min.
Reagent Type Cell Lysis
Starting Material 10-100,000 Cells
For Use With (Application) Gene Expression
PCR Method 1-step RT-qPCR
Product Line Cells-to-CT, TaqMan
Detection Method TaqMan
12

Thermo Scientific™ MuA Transposase (concentrated)

Perform functional analysis of proteins with these transposon-based tools.

13

Invitrogen™ Cells-to-CT™ Stop Solution  DFS Item
GREENER_CHOICE

A component of the Cells-to-CT™ Kits.

14

Thermo Scientific™ GeneJET PCR Purification Kit

Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.

Content And Storage For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C.
Format Spin Column
Run Time 5 min.
High-throughput Compatibility Not High-throughput Compatible (Manual)
15

Invitrogen™ GeneArt™ CRISPR Nuclease Vector with CD4 Enrichment Kit (with competent cells)  DFS Item

Simple, ready-to-use, all-in-one expression vector system for CRISPR-based genome editing.