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Promega™ M-MLV Reverse Transcriptase DFS Item

M-MLV RT is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (>5kb).

Manufacturer:  Promega™ M1705

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Catalog No. PRM1705



Description

Description

  • Exhibits weaker RNase H activity than Avian Myeloblastosis Virus (AMV) reverse transcriptase
  • May be inactivated by heating at 70°C for 10 minutes
  • Produced by the pol gene of Moloney Murine Leukemia Virus
  • Provided with 5X Reaction Buffer (250mM Tris-HCl [pH 8.3 at 25°C], 375mM KCl, 15mM MgCl2, 50mM DTT); buffer also available separately

Specifications

Specifications

200U/μL
Liquid
Tris-HCl (pH 7.5 at 25°C) 20mM, NaCl 0.2M, EDTA 0.1mM, DTT 1mM, 0.01% Nonidet™ P40™ and Glycerol 50%
7.5
M-MLV Reverse Transcriptase
First-strand cDNA synthesis, primer extension
Recombinant E. coli Strain
−30 to −10°C
5X reaction buffer (250mM Tris-HCl [pH 8.3 at 25°C], 375mM KCl, 15mM MgCl2, 50mM DTT), buffer also available separately
50000U
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