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Promega™ M-MLV Reverse Transcriptase

RNA-dependent DNA polymerase can be used in primer extension or cDNA synthesis with >5kb mRNA templates

$20.00 - $279.00

Specifications

Format Liquid
For Use With (Application) First-strand cDNA synthesis, primer extension
Includes 2 x 1mL Buffer pack
Source Recombinant E. coli Strain
Storage Buffer Tris-HCl (pH 7.5 at 25°C) 20mM, NaCl 0.2M, EDTA 0.1mM, DTT 1mM, 0.01% Nonidet™ P40™ and Glycerol 50%
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Products
Catalog Number Mfr. No. Product Name Quantity pH Price Quantity    

PR-M1701

 
promega™
M1701
M-MLV Reverse Transcriptase 10000U 8.3 Each for $73.00

PRM1705

 
promega™
M1705
M-MLV Reverse Transcriptase 50000U 7.5 Each for $279.00

PR-M5313

 
promega™
M5313
M-MLV Reverse Transcriptase Buffer Pack 2 x 1mL 8.3 Each for $20.00
Description & Specifications

Specifications

Format Liquid
For Use With (Application) First-strand cDNA synthesis, primer extension
Includes 2 x 1mL Buffer pack
Source Recombinant E. coli Strain
Storage Buffer Tris-HCl (pH 7.5 at 25°C) 20mM, NaCl 0.2M, EDTA 0.1mM, DTT 1mM, 0.01% Nonidet™ P40™ and Glycerol 50%
Storage Requirements -20°C

  • Exhibits weaker RNase H activity than Avian Myeloblastosis Virus (AMV) reverse transcriptase
  • May be inactivated by heating at 70°C for 10 minutes
  • Produced by the pol gene of Moloney Murine Leukemia Virus
  • Provided with 5X Reaction Buffer (250mM Tris-HCl [pH 8.3 at 25°C], 375mM KCl, 15mM MgCl2, 50mM DTT); buffer also available separately