Thermo Scientific™ Pierce™ RNA 3' End Biotinylation Kit

Description

Thermo Scientific™ Pierce RNA 3' End Biotinylation Kit is optimized for labeling the 3-prime terminal end of RNA probes to facilitate their use as probes or targets in EMSA and other methods for studying protein-RNA interactions.

The complete kit contains an unlabeled RNA strand to use as a positive control, a biotinylated-RNA probe standard, RNase inhibitor, glycogen and ligation-enhancing reagents. The RNA 3' End Biotinylation Kit uses T4 RNA ligase to conjugate a single nucleotide analog to the 3' terminus of an RNA strand. The unique biotinylated cytidine (bis)phosphate used in this kit enables T4 RNA ligase to attach a single biotin tag on RNA probes of diverse secondary structure and lengths with greater than 70% efficiency.

Highlights:

• Non-radioactive – incorporates a biotin label which allows detection sensitivity similar to radioactivity when used with chemiluminescent detection systems

• Fast – RNA can be labeled in 0.5-2 hours with minimal downstream processing

• Easy to use – RNA ligase and optimized reaction buffer are included

• Economical – only a fraction of the cost of purchasing synthetic biotinylated RNA probes

• End-labeled – results in minimal disturbance of RNA secondary structure

• Flexible – label synthetic or in vitro transcribed RNA probes ranging from 22 to 450 nucleotides