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Invitrogen™ CellLight™ Peroxisome-GFP, BacMam 2.0 DFS Item

Provides easy way to label peroxisomes with green fluorescent protein in live cells

Supplier:  Invitrogen™ C10604

Catalog No. C10604


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Description

Description

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses GFP fused to the peroxisomal C terminal targeting sequence. You can observe peroxisome-GFP behavior in live cells and use multiple tracking or tracing dyes to image dynamic cellular processes.

Cells expressing CellLight constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

BacMam Technology

CellLight Peroxisome-GFP, BacMam 2.0, is a fusion construct of peroxisomal C terminal targeting sequence and emGFP, providing accurate and specific targeting to cellular peroxisome-GFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

  • Fast and convenient: simply add CellLight reagent to cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
  • Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
  • Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
  • Less toxic: CellLight reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling
  • Flow Cytometer Laser Lines: 488

Cell Analysis, Cell Structure, Cell Tracing & Tracking, Cellular Imaging, Immunocytochemistry (ICC), Immunofluorescence (IF), Organelle Tracing, Peroxisomes

Order Info

Shipping Condition: Wet Ice

Greener Choice Claims
  • Environmental benefits include:
    • Recyclable
Specifications

Specifications

Green
488⁄510
Confocal Microscope, Fluorescence Microscope
Fluorescence
Liquid
Wet Ice
Fluorescent Dye
Peroxisomes
Store at 2°C to 6°C, protected from light. Do Not Freeze.
GFP (EmGFP)
1 mL
Visible
CellLight™
Fluorescence Intensity
Peroxisome Label
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For Research Use Only. Not for use in diagnostic procedures.