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Applied Biosystems™ MagMAX™-96 AI/ND Viral RNA Isolation Kit
Description
The MagMAX™-96 AI/ND Viral RNA Isolation Kit is a first generation MagMAX™ Viral RNA Isolation Kit validated by the NVSL for use with AI/ND viruses. Perfect for working with low viral concentrations, the kit contains sufficient reagents for 4 x 96 reactions.
- Validated by the NVSL for use with AI/ND viruses
- Recover as few as 10 copies of viral RNA from 100 to 400 μL sample
- Typical viral RNA recovery of >50%
- Ideal when working with low viral concentrations
- Linear recovery down to 50 transcripts
- 96 samples can be processed in 30 minutes
- Easily integrated into robotic platforms
Benefits of MagMAX™ Magnetic Bead-Based Purification:
Magnetic beads offer many benefits compared to other technologies for isolating RNA. Beads bind RNA more efficiently than glass fiber filters, resulting in higher and more consistent RNA yields. Additionally, because filters are not used, there is no risk of filter clogging due to cellular particulates in samples. Since only a small volume of magnetic beads is needed for high-efficiency binding, the bound RNA can be eluted in just 20 to 50 μL of nuclease-free water, concentrating RNA from large, dilute samples.
Optimized for Isolation of Virus from Biofluids:
The Applied Biosystems™ MagMAX™ Viral RNA Isolation Kit has been optimized for use with biological fluids and cell-free samples such as serum, plasma, swabs, and cell culture media. These samples often present unique challenges when purifying RNA, including low viral titers, large volumes to be processed, and sample complexity. Without the need for organic solvents or RNA precipitation, the MagMAX™ Viral RNA isolation kit eliminates the problems often encountered with other RNA isolation methods such filter clogging, large elution volumes, and inconsistent yields.
Get the Same Amount of RNA from 10-fold Less Sample
The sensitivity of viral RNA detection was compared using RNA isolated with Applied Biosystems™ MagMAX™ protocol or the current National Veterinary Services Laboratories (NVSL) validated viral testing protocol. Even at one-tenth the sample input, viral RNA isolated using the MagMAX™ kit could be detected at equal or greater levels than RNA isolated using the NVSL protocol and recommended competitor's kit.
Accessory Products:
Carrier RNA (SKU#4382878), Lysis/Binding Solution Concentrate (SKU#AM8500), Wash Solution 1 Concentrate (SKU#AM8504), and Wash Solution 2 Concentrate (SKU#AM8640) are components of the MagMAX™ Viral RNA Isolation Kits that may also be purchased separately. A 96-well magnetic ring stand is required to use this kit. The Magnetic Stand-96 (SKU#AM10027) and the 96-well Magnetic Ring Stand (SKU#AM10050) are designed for paramagnetic bead precipitation from standard 96 well U-bottom microplates. The Magnetic-Ring Stand pellets the magnetic beads in a circle shape, enabling easy removal of supernatant by a robot or multichannel pipettors. For additional processing plates, Matrix 96-Well U Bottom Polypropylene Plates (Thermo Scientific, Item# 4917) are recommended. The MagMAX™ and MagMAX™-96 Viral RNA Isolation Kits (SKU#AM1939 and SKU#AM1836) are the newest generation MagMAX™ kits for this application, but are not yet validated by the NVSL.
Specifications
Specifications
| Content And Storage | Carrier RNA is stored at –20°C; RNA Binding Beads are stored at 4°C. Wash Solution 2 Concentrate and Elution Buffer may be stored at either 4°C or room temperature. Lysis⁄Binding Solution Concentrate, Wash Solution 1 Concentrate, RNA Rebinding Concentrate, Bead Resuspension Solution, Processing Plates and Lids, and PBS Buffer are stored at room temperature. Nuclease-free water may be stored at any temperature. |
| Format | 96-well Plate |
| Isolation Technology | Magnetic Bead |
| Purification Time | 1 hr. |
| Sample Type | Liquid Samples (e.g. Serum), Viral Samples |
| Final Product Type | Viral RNA |
| High-throughput Compatibility | High-throughput Compatible, Not High-throughput Compatible (Manual), Automated Protocols |
| For Use With (Equipment) | KingFisher™ Flex Magnetic Particle Processor, KingFisher 96, MagMAX™ Express-96 Standard Magnetic Particle Processor |
| For Use With (Application) | Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting |
| No. of Reactions | 384 preps |
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Frequently Asked Questions (FAQs)
Cat. No. AM1836 is a good choice if you want to process 50 µL of samples. If you want to maximize the input amount and use more than that (up to 300 µL) we recommend using the MagMAX RNA/DNA Pathogen kit (Cat. No. 4462359). The buffers and reagents included in these MagMAX kits are identical, but the volumes are larger for the MagMAX Pathogen RNA/DNA kit allowing for more sample input.
Some customers have isolated RNA from viruses. Unfortunately, the protein coat could cause problems. Another option would be to use the MagMax Viral Isolation Kit.
Only MagMax96 for Microarrays Total RNA Isolation Kit (Cat. No. AM1839) is compatible, since it uses Trizol reagent and most of the salt carry-over from RNAlater reagent is eliminated during the lysis steps and partitioned from the aqueous RNA layer on top. For other MagMax kits, optimization is needed. High salt carry over is not optimal for MagMax RNA isolation steps.
Some bead carryover is common and has not been documented to affect downstream assays. The most common cause of bead carryover is too much input sample. The best way to determine if the sample amount is the cause of the issue is to run PBS with Xeno (artificial DNA/RNA) in the lysis buffer and then check if there is still bead carryover. If that is not the cause, the initial sample may require special processing, such as bead beating or liquification. If there is still bead carryover without the original sample, and in particular, if the carryover is present in the same wells for multiple runs, then this may be an instrument alignment issue.
Plastic consumables need to be purchased separately in order to use the MagMAX reagents on the MagMAX Express-96 or a KingFisher instrument. The particular consumables and the amount required will vary by kit and protocol so please check the user guide for the particular MagMAX kit to be sure that all required plastic consumables are available prior to sample processing.
For Veterinary Use Only.