For efficient integration into Flp-In cell lines
Supplier: Invitrogen™ V600520
Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Targeted Integration
| Constitutive | |
| CMV | |
| None | |
| Untagged | |
| 20 μg | |
| Flp-In™ |
| Transfection | |
| Flp-In System Expression Vector | |
| The pcDNA™5/FRT/V5-His TOPO™ TA Expression Kit, pSecTag/FRT/V5-His TOPO™ TA Expression Kit, and the pEF5/FRT/V5 Directional TOPO™ Expression Kit contain two boxes. The TOPO™ Cloning box contains all of the reagents necessary to clone a Taq-amplified PCR product (TOPO™ TA) or a PCR product generated with a proofreading enzyme (Directional TOPO™), including 200 ng of topoisomerase Iactivated vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers, primers for sequencing, and an expression control plasmid. Store at -20°C. The One Shot™ Competent Cell box contains all of the reagents necessary for transformation, including twenty-one 50-μl aliquots of One Shot™ TOP10 Chemically Competent E. coli, S.O.C. medium, and pUC19 supercoiled control plasmid. Store at -80°C. The pEF5/FRT/V5-DEST™ vector contains 6 μg of lyophilized vector and a control plasmid. Store at -20°C once resuspended. All reagents are guaranteed stable for 6 months when properly stored. | |
| TOPO, Gateway | |
| pOG44 | |
| Targeted Integration |
For Research Use Only. Not for use in diagnostic procedures.
