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  5. V652020

Invitrogen™ pcDNA™5/FRT/TO Vector Kit DFS Item

5.1kb inducible expression vector features multiple cloning site with 10 unique restriction sites to facilitate cloning gene of interest

Supplier:  Invitrogen™ V652020

Catalog No. V652020


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Includes: 20μg of each: pcDNAFRT/TO and pcDNAFRT/TO/CAT vectors.

Description

Description

  • Control plasmid, pcDNA5/FRT/TO/CAT, is also included for use as positive control for transfection and expression in cell line of choice
  • Hybrid human cytomegalovirus (CMV)/TetO2 promoter for high-level, tetracycline-regulated expression of gene of interest in wide range of mammalian cells
  • FLP Recombination Target (FRT) site for Flp recombinase-mediated integration of vector into Flp-InT-REx host cell line
  • Hygromycin resistance gene for selection of stable cell lines

Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Regulated Expression, Targeted Integration

Specifications

Specifications

Inducible
Tetracycline
Flp-In/T-REx System Expression Vector
Contains 20 μg of each: pcDNA™FRT⁄TO and pcDNA™FRT⁄TO⁄CAT vectors. Store both vectors at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Restriction Enzyme/MCS
pcDNA
Regulated Expression
Transfection
CMV/TO
Hygromycin
Untagged
20 μg
Flp-In™, T-REx™, pcDNA™
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Product Information

Manuals

pcDNA5/FRT/TO Version: Version G 11/11/10 File size: 354.8 kB
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For Research Use Only. Not for use in diagnostic procedures.