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Invitrogen™ pcDNA™5/FRT/TO Vector Kit
5.1kb inducible expression vector features multiple cloning site with 10 unique restriction sites to facilitate cloning gene of interest
Supplier: Invitrogen™ V652020
Includes: 20μg of each: pcDNAFRT/TO and pcDNAFRT/TO/CAT vectors.
Description
- Control plasmid, pcDNA5/FRT/TO/CAT, is also included for use as positive control for transfection and expression in cell line of choice
- Hybrid human cytomegalovirus (CMV)/TetO2 promoter for high-level, tetracycline-regulated expression of gene of interest in wide range of mammalian cells
- FLP Recombination Target (FRT) site for Flp recombinase-mediated integration of vector into Flp-InT-REx host cell line
- Hygromycin resistance gene for selection of stable cell lines
Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Regulated Expression, Targeted Integration
Greener Choice Claims
- Environmental benefits include:
- Renewable Energy
Specifications
Inducible | |
Tetracycline | |
Flp-In/T-REx System Expression Vector | |
Contains 20 μg of each: pcDNA™FRT⁄TO and pcDNA™FRT⁄TO⁄CAT vectors. Store both vectors at -20°C. All reagents are guaranteed stable for 6 months when properly stored. | |
Restriction Enzyme/MCS | |
pcDNA | |
Regulated Expression |
Transfection | |
CMV/TO | |
Hygromycin | |
Untagged | |
20 μg | |
Flp-In™, T-REx™, pcDNA™ |
pcDNA5/FRT/TO
Version: Version G 11/11/10
File size: 354.8 kB
For Research Use Only. Not for use in diagnostic procedures.