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Invitrogen™ Colorimetric ReadiLAMP™ Kit, SARS-CoV-2 DFS Item

The Invitrogen Colorimetric ReadiLAMP Kit for SARS-CoV-2 is a loop-mediated isothermal amplification (LAMP)-based solution for rapid SARS-CoV-2 detection in saliva and nasopharyngeal/nasal swab samples.

Supplier:  Invitrogen™ A52544

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Catalog No. A52544



Description

Description

  • The Invitrogen Colorimetric ReadiLAMP Kit for SARS-CoV-2 is a loop-mediated isothermal amplification (LAMP)-based solution for rapid SARS-CoV-2 detection in saliva and nasopharyngeal/nasal swab samples
  • The first protocol option provides a workflow for RNA purification followed by a colorimetric LAMP assay
    • For robust SARS-CoV-2 RNA isolation, the MagMAX Viral/Pathogen II Nucleic Acid Isolation Kit (Cat. No. A48383) or PureLink Viral RNA/DNA Mini Kit (Cat. No. 12280050) are recommended
  • The second protocol option consists of a simple SARS-CoV-2 viral lysis method for compatible sample types followed by a colorimetric LAMP assay
    • This protocol does not require nucleic acid isolation or complex sample preparation; instead it directly incorporates the saliva or nasopharyngeal/nasal swab samples as LAMP assay input

Features of the Colorimetric ReadiLAMP Kit for SARS-CoV-2 include:

  • Simplicity—Detection is fast, simple, and intuitive; LAMP reaction changes color from purple to blue if SARS target is present
    • No instrumentation required, except for a 65 heating block
  • Sensitivity—Input of up to 50% volume of purified RNA per LAMP reaction, enabling maximal sensitivity in SARS-CoV-2 detection
    • Direct LAMP: ≤250 copies per reaction (25 μL)
    • Purified RNA-based LAMP: ≤100 copies per reaction (25 μL)
  • Speed—Both protocols can be completed within 1 hour
    • Direct LAMP: ∼30 minutes using crude saliva or swabs in non-serum-containing transport media
    • Purified RNA-based LAMP: ∼60 minutes (∼30 minutes RNA purification, followed by 30 minutes LAMP assay)
  • Throughput—Process between 1 and 96 samples at a time, enabling the processing of more samples with same personnel headcount, using individual tubes, tube strips, or 96-wells
Specifications

Specifications

Kit does not contain beads. Recommending the use of RNA extraction MVP II kit which contains magnetic beads
During reaction color changes from purple to blue
Saliva or Nasopharyngeal/Nasal Swab
SARS-CoV-2 surveilance
Heating block
Detects SARS-CoV-2 presence
SARS-CoV-2 presence
<250 copies/25 μL (Direct LAMP)
<100 copies/25 μL (Purified RNA-based LAMP)
Colorimetric readout
SuperScript IV & proprietary Bst-derived DNA polymerase
Liquid. 5 components included in the kit.
LAMP Buffer (5x), Enzyme Mix (10x), SARS-CoV-2 primer mix (5x), Human Control primer mix (5x), SARS-CoV-2 control RNA
Range of sample inputs
1000
SDS
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