Invitrogen™ Human Insulin Recombinant Protein

Description

This is a recombinant protein suitable for use as a control in ELISA and Western blot applications. Lyophilized Insulin is stable at room temperature for 3 weeks. Reconstitute in sterile 0.005N HCl to a concentration of no more than 1mg/mL. Store lyophilized product at -20°C. Upon reconstitution, store at 4°C for 2-7 days or aliquot and store at -20°C. For long term storage add either HSA or BSA to 0.1%. Avoid repeated freeze-thaw cycles.

Insulin is a peptide hormone secreted by beta cells of the pancreatic islets. It regulates carbohydrate, protein and lipid metabolism by enhancing membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Insulin is 51-amino acid polypeptide product produced from a precursor peptide, proinsulin. Proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds and one molecule of C-peptide. Insulin deficiency results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of b-cell origin such as insulinoma.